Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LK774_RS18585 Genome accession   NZ_CP088229
Coordinates   3891084..3892064 (-) Length   326 a.a.
NCBI ID   WP_023331279.1    Uniprot ID   -
Organism   Enterobacter kobei strain Ek72     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3886084..3897064
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LK774_RS18555 (LK774_18590) - 3886973..3887470 (+) 498 WP_032629486.1 SprT family zinc-dependent metalloprotease -
  LK774_RS18560 (LK774_18595) endA 3887565..3888272 (+) 708 WP_023331276.1 deoxyribonuclease I -
  LK774_RS18565 (LK774_18600) rsmE 3888324..3889055 (+) 732 WP_023331277.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  LK774_RS18570 (LK774_18605) gshB 3889075..3890022 (+) 948 WP_023331278.1 glutathione synthase -
  LK774_RS18575 (LK774_18610) - 3890097..3890657 (+) 561 WP_008499752.1 YqgE/AlgH family protein -
  LK774_RS18580 (LK774_18615) ruvX 3890657..3891073 (+) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  LK774_RS18585 (LK774_18620) pilT 3891084..3892064 (-) 981 WP_023331279.1 type IV pilus twitching motility protein PilT Machinery gene
  LK774_RS18590 (LK774_18625) - 3892082..3892783 (+) 702 WP_014885191.1 YggS family pyridoxal phosphate-dependent enzyme -
  LK774_RS18595 (LK774_18630) - 3892805..3893371 (+) 567 WP_014885192.1 YggT family protein -
  LK774_RS18600 (LK774_18635) yggU 3893368..3893664 (+) 297 WP_014885193.1 DUF167 family protein YggU -
  LK774_RS18605 (LK774_18640) - 3893668..3894261 (+) 594 WP_023331280.1 XTP/dITP diphosphatase -
  LK774_RS18610 (LK774_18645) hemW 3894254..3895396 (+) 1143 WP_023331281.1 radical SAM family heme chaperone HemW -
  LK774_RS18615 (LK774_18650) - 3895460..3895798 (+) 339 WP_023331282.1 endonuclease domain-containing protein -
  LK774_RS18620 (LK774_18655) - 3895849..3896565 (-) 717 WP_014885197.1 DUF2884 domain-containing protein -
  LK774_RS18625 (LK774_18660) - 3896622..3896948 (-) 327 WP_014885198.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35518.50 Da        Isoelectric Point: 6.5851

>NTDB_id=633373 LK774_RS18585 WP_023331279.1 3891084..3892064(-) (pilT) [Enterobacter kobei strain Ek72]
MDVGEMVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVGALLKAWLNDEQQGSWWAHGQVDFAVTVAEGQRLRG
SAFKQINGVSIALRLLPRTCSPLSSLGVPRAIPELLSSDNGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
YLYRSERCLIQQREIGQHCPSFVEALRGALREDPDVILLGELRDAETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQRLLPDLQGGRVALYELLVNTPAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSVAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=633373 LK774_RS18585 WP_023331279.1 3891084..3892064(-) (pilT) [Enterobacter kobei strain Ek72]
ATGGATGTGGGAGAAATGGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCCCG
CTGGCGCCGGTCAGGACGCCTTGAACCCGCGCCGTTTCCGCCGCCTGATGTCGGGGCGTTATTAAAAGCGTGGCTCAATG
ATGAGCAGCAGGGAAGCTGGTGGGCTCACGGTCAGGTCGATTTTGCCGTGACGGTGGCAGAAGGCCAGCGTCTGCGCGGG
AGTGCGTTTAAGCAGATTAACGGGGTGTCCATCGCCCTGCGGTTGCTGCCACGCACCTGTTCGCCACTCTCTTCGCTGGG
CGTGCCGCGTGCCATTCCGGAACTGTTATCCAGTGACAACGGGTTGATTCTGGTGACGGGGGCGACCGGCAGCGGCAAAT
CCACCACTCTGGCGGCGATGGTCGATTTTCTCAACCACCATACTGACGGGCATATCCTCACGCTTGAAGATCCGGTGGAG
TATCTGTACCGGAGCGAACGCTGCCTGATTCAGCAGCGGGAAATAGGTCAGCACTGCCCGTCTTTTGTCGAGGCGCTGCG
CGGAGCGTTACGCGAGGATCCAGATGTGATTTTGCTGGGGGAGCTGCGTGATGCAGAGACGATCCGTCTCGCGTTGACGG
CGGCGGAGACCGGGCATCTGGTGCTGGCGACACTGCATACGCGGGGTGCCTCGCAGGCCATTGAGCGGCTGGTGGACACC
TTCCCGGCGCAGGAGAAAGACCCGGTACGTAACCAGCTGGCCGGTAGCCTGCGTGCGGTGCTGGCACAGAGGCTGCTTCC
CGACCTGCAGGGTGGGCGTGTCGCGCTGTATGAACTGCTGGTGAATACCCCGGCGGCGGCAAATCTGATTCGAGAAGGAA
AAACGTGGCAACTGCCCGGTATCATTCAAACAGGCCAGCAGGCGGGAATGCAGAACTTTGATCAAAGCGTGGCGGAGAGA
CGGGCGCAGGGGCGGCTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(73-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.235

100

0.494
  pilT Vibrio cholerae strain A1552

49.235

100

0.494
  pilT Neisseria meningitidis 8013

46.041

100

0.482
  pilT Legionella pneumophila strain ERS1305867

48.447

98.773

0.479
  pilT Legionella pneumophila strain Lp02

48.447

98.773

0.479
  pilT Pseudomonas stutzeri DSM 10701

47.706

100

0.479
  pilT Acinetobacter baylyi ADP1

47.401

100

0.475
  pilT Acinetobacter baumannii D1279779

47.095

100

0.472
  pilT Acinetobacter baumannii strain A118

47.095

100

0.472
  pilT Pseudomonas aeruginosa PAK

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

47.5

98.16

0.466
  pilT Neisseria gonorrhoeae MS11

46.341

100

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.857

98.773

0.423
  pilU Vibrio cholerae strain A1552

38.82

98.773

0.383
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38