Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LRM31_RS18895 Genome accession   NZ_CP088119
Coordinates   3991945..3992925 (-) Length   326 a.a.
NCBI ID   WP_045134812.1    Uniprot ID   -
Organism   Enterobacter kobei strain 6     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3986945..3997925
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LRM31_RS18865 (LRM31_18865) - 3987834..3988331 (+) 498 WP_014885186.1 SprT family zinc-dependent metalloprotease -
  LRM31_RS18870 (LRM31_18870) endA 3988426..3989133 (+) 708 WP_241448883.1 deoxyribonuclease I -
  LRM31_RS18875 (LRM31_18875) rsmE 3989185..3989916 (+) 732 WP_023331277.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  LRM31_RS18880 (LRM31_18880) gshB 3989936..3990883 (+) 948 WP_023331278.1 glutathione synthase -
  LRM31_RS18885 (LRM31_18885) - 3990958..3991518 (+) 561 WP_047028068.1 YqgE/AlgH family protein -
  LRM31_RS18890 (LRM31_18890) ruvX 3991518..3991934 (+) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  LRM31_RS18895 (LRM31_18895) pilT 3991945..3992925 (-) 981 WP_045134812.1 type IV pilus twitching motility protein PilT Machinery gene
  LRM31_RS18900 (LRM31_18900) - 3992943..3993644 (+) 702 WP_014885191.1 YggS family pyridoxal phosphate-dependent enzyme -
  LRM31_RS18905 (LRM31_18905) - 3993666..3994232 (+) 567 WP_014885192.1 YggT family protein -
  LRM31_RS18910 (LRM31_18910) yggU 3994229..3994525 (+) 297 WP_014885193.1 DUF167 family protein YggU -
  LRM31_RS18915 (LRM31_18915) - 3994529..3995122 (+) 594 WP_023331280.1 XTP/dITP diphosphatase -
  LRM31_RS18920 (LRM31_18920) hemW 3995115..3996257 (+) 1143 WP_023338778.1 radical SAM family heme chaperone HemW -
  LRM31_RS18925 (LRM31_18925) - 3996321..3996659 (+) 339 WP_023331282.1 endonuclease domain-containing protein -
  LRM31_RS18930 (LRM31_18930) - 3996710..3997426 (-) 717 WP_014885197.1 DUF2884 domain-containing protein -
  LRM31_RS18935 (LRM31_18935) - 3997484..3997810 (-) 327 WP_014885198.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35604.59 Da        Isoelectric Point: 6.4113

>NTDB_id=632729 LRM31_RS18895 WP_045134812.1 3991945..3992925(-) (pilT) [Enterobacter kobei strain 6]
MDVEEMVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVGALLKAWLNDEQQGSWWAHGQVDFAVTVAEGQRLRG
SAFKQINGVSIALRLLPRTCSPLSSLGVPRAIPELLSSDNGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
YLYRSERCLIQQREIGQHCPSFIEALRGALREDPDVILLGELRDAETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQRLLPDLQGGRVALYELLVNTPAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSVAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=632729 LRM31_RS18895 WP_045134812.1 3991945..3992925(-) (pilT) [Enterobacter kobei strain 6]
ATGGATGTGGAAGAAATGGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCCCG
CTGGCGCCGGTCAGGACGCCTTGAACCCGCGCCGTTTCCGCCTCCAGATGTCGGGGCGTTATTAAAAGCGTGGCTCAATG
ATGAGCAGCAGGGAAGCTGGTGGGCTCACGGTCAGGTCGATTTTGCCGTGACGGTGGCAGAAGGGCAGCGTCTGCGCGGG
AGTGCGTTTAAGCAGATTAACGGGGTGTCCATCGCCCTGCGGTTGCTGCCACGCACCTGTTCGCCACTCTCTTCGCTGGG
CGTGCCGCGTGCGATTCCGGAACTGTTATCCAGTGACAACGGGTTGATTCTGGTGACGGGGGCGACCGGCAGCGGCAAAT
CCACCACTCTGGCGGCGATGGTCGATTTTCTCAACCACCATACTGACGGGCATATCCTCACGCTTGAAGATCCGGTGGAG
TATCTGTACCGGAGCGAACGCTGCCTGATTCAGCAGCGGGAGATAGGTCAGCACTGCCCGTCTTTTATCGAGGCGCTGCG
CGGAGCGTTACGCGAGGATCCAGATGTGATTTTGCTGGGGGAGCTGCGTGATGCAGAGACGATCCGTCTCGCGTTGACGG
CGGCGGAGACCGGGCATCTGGTGCTGGCGACACTGCATACGCGGGGTGCCTCGCAGGCCATTGAGCGGCTGGTGGACACC
TTCCCGGCGCAGGAGAAAGACCCGGTACGTAACCAGCTGGCCGGTAGCCTGCGTGCGGTGCTGGCACAGAGGCTGCTTCC
CGACCTGCAGGGTGGGCGTGTCGCGCTGTATGAACTGCTGGTGAATACCCCGGCGGCGGCAAATCTGATTCGTGAAGGAA
AAACGTGGCAACTGCCCGGTATCATTCAAACAGGCCAGCAGGCGGGAATGCAGAACTTTGATCAAAGCGTGGCGGAGAGA
CGGGCGCAGGGGCGGCTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(73-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.235

100

0.494
  pilT Vibrio cholerae strain A1552

49.235

100

0.494
  pilT Neisseria meningitidis 8013

46.041

100

0.482
  pilT Legionella pneumophila strain ERS1305867

48.447

98.773

0.479
  pilT Legionella pneumophila strain Lp02

48.447

98.773

0.479
  pilT Pseudomonas stutzeri DSM 10701

47.706

100

0.479
  pilT Acinetobacter baylyi ADP1

47.401

100

0.475
  pilT Acinetobacter baumannii D1279779

47.095

100

0.472
  pilT Acinetobacter baumannii strain A118

47.095

100

0.472
  pilT Pseudomonas aeruginosa PAK

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

47.5

98.16

0.466
  pilT Neisseria gonorrhoeae MS11

46.341

100

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.857

98.773

0.423
  pilU Pseudomonas stutzeri DSM 10701

37.879

100

0.383
  pilU Vibrio cholerae strain A1552

38.82

98.773

0.383