Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LOY37_RS01335 Genome accession   NZ_CP087197
Coordinates   297057..298085 (+) Length   342 a.a.
NCBI ID   WP_258716171.1    Uniprot ID   -
Organism   Pseudomonas sp. B21-012     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 292057..303085
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY37_RS01305 (LOY37_01305) - 292494..292928 (-) 435 WP_195885461.1 DUF4426 domain-containing protein -
  LOY37_RS01310 (LOY37_01310) metW 292953..293573 (-) 621 WP_038997652.1 methionine biosynthesis protein MetW -
  LOY37_RS01315 (LOY37_01315) - 293581..294720 (-) 1140 WP_045193963.1 homoserine O-acetyltransferase -
  LOY37_RS01320 (LOY37_01320) - 294846..295436 (-) 591 WP_045193962.1 YggT family protein -
  LOY37_RS01325 (LOY37_01325) proC 295447..296265 (-) 819 WP_258716170.1 pyrroline-5-carboxylate reductase -
  LOY37_RS01330 (LOY37_01330) - 296304..296990 (-) 687 WP_213602719.1 YggS family pyridoxal phosphate-dependent enzyme -
  LOY37_RS01335 (LOY37_01335) pilT 297057..298085 (+) 1029 WP_258716171.1 type IV pilus twitching motility protein PilT Machinery gene
  LOY37_RS01340 (LOY37_01340) - 298082..298717 (-) 636 WP_045193958.1 C40 family peptidase -
  LOY37_RS01345 (LOY37_01345) - 298964..299383 (+) 420 WP_045193957.1 TM2 domain-containing protein -
  LOY37_RS01350 (LOY37_01350) - 299432..300121 (-) 690 WP_038997644.1 SPOR domain-containing protein -
  LOY37_RS01355 (LOY37_01355) argS 300123..301859 (-) 1737 WP_258608909.1 arginine--tRNA ligase -

Sequence

Protein


Download         Length: 342 a.a.        Molecular weight: 36546.04 Da        Isoelectric Point: 6.3424

>NTDB_id=627894 LOY37_RS01335 WP_258716171.1 297057..298085(+) (pilT) [Pseudomonas sp. B21-012]
MDVTELLALAVQAGASDLHLAAGEVPALRFGGCLRPLELPALTPQDMDEMLGQVMSAAQQKDFETHLDIDFAFAVPALAR
FRVNAFQQLHGAAAVLRVIPAQVSSLAELDLGDVFRQIAELSGGLVLVTGPTGSGKSTTLAALVDHLNRERALHIVTLED
PIEFIHSSQRSRISQREIGRHTRGFAQALRAALRQDPDVIMLAELRDPQSIRLALTAAETGHLVLATVHTGSAAKTIDRL
VDVFAAEEKQLVRAMLAESLQAVVSQVLVEKVGGGRLAAHEILLATPAVRNLVREGKVAQIYSAIQTGGAQGMQTLDACL
QGLVRQGLVSPESARERAPGRF

Nucleotide


Download         Length: 1029 bp        

>NTDB_id=627894 LOY37_RS01335 WP_258716171.1 297057..298085(+) (pilT) [Pseudomonas sp. B21-012]
ATGGATGTGACTGAACTGCTGGCGCTGGCTGTGCAAGCCGGTGCCAGTGACCTGCACCTGGCGGCGGGCGAGGTGCCGGC
GCTGCGTTTTGGGGGGTGTTTGCGGCCGCTGGAGTTGCCGGCGCTGACGCCGCAAGACATGGACGAAATGCTCGGGCAGG
TAATGTCCGCGGCGCAGCAAAAGGATTTTGAAACACATCTGGATATCGACTTTGCCTTTGCCGTGCCAGCATTGGCGCGC
TTTCGGGTCAATGCTTTCCAGCAACTCCATGGCGCCGCTGCAGTGCTGCGGGTGATTCCGGCGCAGGTCAGCAGCCTGGC
AGAACTCGACCTGGGGGATGTGTTTCGGCAAATTGCCGAGCTGTCGGGCGGGCTGGTGCTGGTTACCGGGCCCACAGGGT
CGGGCAAATCGACCACCCTGGCGGCTCTGGTCGATCACCTGAACCGTGAGCGGGCGCTGCACATCGTCACCCTCGAGGAC
CCCATCGAATTTATCCACAGCTCGCAGCGCAGCCGCATCAGCCAGCGCGAGATCGGCCGGCACACCCGGGGTTTTGCCCA
GGCCTTGCGCGCAGCATTACGCCAGGATCCGGATGTGATCATGCTCGCTGAACTGCGCGATCCGCAGAGCATTCGCCTGG
CCCTGACTGCAGCGGAAACCGGGCACCTGGTATTGGCCACGGTGCATACCGGCTCGGCGGCGAAAACCATTGACCGCCTG
GTGGATGTGTTCGCCGCCGAAGAGAAGCAACTGGTACGCGCCATGCTCGCCGAGTCGCTGCAGGCAGTGGTCTCGCAGGT
TCTGGTGGAGAAGGTTGGCGGCGGCCGGCTGGCAGCCCACGAGATACTCCTGGCAACGCCTGCGGTGCGCAACCTGGTGC
GCGAGGGCAAGGTGGCGCAGATTTATTCGGCGATACAGACAGGCGGGGCACAGGGGATGCAGACCCTCGACGCGTGTCTG
CAGGGCCTGGTGCGTCAAGGGCTGGTCAGCCCTGAAAGCGCCCGGGAGCGGGCGCCGGGGCGTTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

59.884

100

0.602
  pilT Pseudomonas stutzeri DSM 10701

59.593

100

0.599
  pilT Acinetobacter nosocomialis M2

59.403

97.953

0.582
  pilT Acinetobacter baumannii D1279779

59.403

97.953

0.582
  pilT Acinetobacter baumannii strain A118

59.403

97.953

0.582
  pilT Acinetobacter baylyi ADP1

57.558

100

0.579
  pilT Neisseria meningitidis 8013

56.342

99.123

0.558
  pilT Neisseria gonorrhoeae MS11

56.047

99.123

0.556
  pilT Legionella pneumophila strain Lp02

54.252

99.708

0.541
  pilT Legionella pneumophila strain ERS1305867

54.252

99.708

0.541
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.006

98.538

0.532
  pilT Vibrio cholerae strain A1552

54.006

98.538

0.532
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.853

99.123

0.494
  pilU Pseudomonas stutzeri DSM 10701

38.507

97.953

0.377
  pilU Acinetobacter baylyi ADP1

36.95

99.708

0.368