Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   LOY31_RS24705 Genome accession   NZ_CP087192
Coordinates   5502598..5503020 (-) Length   140 a.a.
NCBI ID   WP_007959597.1    Uniprot ID   J3F1C9
Organism   Pseudomonas sp. B21-021     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5497598..5508020
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY31_RS24690 (LOY31_24670) - 5498529..5501480 (+) 2952 WP_258712315.1 DUF748 domain-containing protein -
  LOY31_RS24695 (LOY31_24675) - 5501480..5501776 (+) 297 WP_258712316.1 DUF2845 domain-containing protein -
  LOY31_RS24700 (LOY31_24680) - 5501944..5502300 (-) 357 WP_007959598.1 BON domain-containing protein -
  LOY31_RS24705 (LOY31_24685) pilA/pilAI 5502598..5503020 (-) 423 WP_007959597.1 pilin Machinery gene
  LOY31_RS24710 (LOY31_24690) pilB 5503247..5504947 (+) 1701 WP_221535412.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LOY31_RS24715 (LOY31_24695) pilC 5504950..5506167 (+) 1218 WP_258712317.1 type II secretion system F family protein Machinery gene
  LOY31_RS24720 (LOY31_24700) pilD 5506169..5507041 (+) 873 WP_258712318.1 A24 family peptidase Machinery gene
  LOY31_RS24725 (LOY31_24705) coaE 5507038..5507661 (+) 624 WP_007959586.1 dephospho-CoA kinase -
  LOY31_RS24730 (LOY31_24710) yacG 5507658..5507858 (+) 201 WP_007959585.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 14045.18 Da        Isoelectric Point: 8.4918

>NTDB_id=627763 LOY31_RS24705 WP_007959597.1 5502598..5503020(-) (pilA/pilAI) [Pseudomonas sp. B21-021]
MKKQQGFTLIELLIVVAIIGILATVALPQYSKYQARSKVTAGLAEISAMKVPFEDIINQGTNPTLAAVKGDAGTTTSNCA
LTASGTAADGAGSIVCTLANAPAPVVGKTITLTRTAANGWSCATTVAPDYSPKGCAASGT

Nucleotide


Download         Length: 423 bp        

>NTDB_id=627763 LOY31_RS24705 WP_007959597.1 5502598..5503020(-) (pilA/pilAI) [Pseudomonas sp. B21-021]
ATGAAGAAACAACAAGGTTTCACTCTGATCGAGCTGCTGATCGTTGTGGCGATCATCGGTATTCTCGCGACTGTGGCGCT
GCCGCAGTACTCCAAATACCAGGCGCGGTCGAAGGTGACGGCGGGGCTGGCCGAGATCTCGGCGATGAAAGTGCCGTTCG
AAGACATCATCAATCAAGGAACGAACCCCACACTGGCTGCCGTTAAAGGCGATGCGGGCACTACCACCTCGAACTGTGCG
CTGACCGCCTCGGGCACCGCTGCTGATGGAGCGGGCAGCATCGTTTGTACGCTGGCCAATGCGCCAGCACCCGTGGTGGG
CAAAACCATTACGCTCACGCGTACTGCTGCCAACGGCTGGTCGTGTGCAACGACCGTTGCGCCGGACTACTCCCCTAAAG
GCTGTGCAGCAAGCGGTACCTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-135)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB J3F1C9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

51.095

97.857

0.5
  pilA Acinetobacter baumannii strain A118

46.043

99.286

0.457
  comP Acinetobacter baylyi ADP1

42.105

100

0.457
  pilA Vibrio cholerae strain A1552

41.29

100

0.457
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

41.29

100

0.457
  pilA Vibrio cholerae C6706

41.29

100

0.457
  pilA Pseudomonas aeruginosa PAK

39.744

100

0.443
  pilA/pilAII Pseudomonas stutzeri DSM 10701

44.444

96.429

0.429
  pilA2 Legionella pneumophila str. Paris

41.429

100

0.414
  pilA Vibrio parahaemolyticus RIMD 2210633

42.963

96.429

0.414
  pilA2 Legionella pneumophila strain ERS1305867

40.714

100

0.407
  pilA/pilA1 Eikenella corrodens VA1

35.172

100

0.364