Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LOY31_RS24710 Genome accession   NZ_CP087192
Coordinates   5503247..5504947 (+) Length   566 a.a.
NCBI ID   WP_221535412.1    Uniprot ID   -
Organism   Pseudomonas sp. B21-021     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5498247..5509947
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY31_RS24690 (LOY31_24670) - 5498529..5501480 (+) 2952 WP_258712315.1 DUF748 domain-containing protein -
  LOY31_RS24695 (LOY31_24675) - 5501480..5501776 (+) 297 WP_258712316.1 DUF2845 domain-containing protein -
  LOY31_RS24700 (LOY31_24680) - 5501944..5502300 (-) 357 WP_007959598.1 BON domain-containing protein -
  LOY31_RS24705 (LOY31_24685) pilA/pilAI 5502598..5503020 (-) 423 WP_007959597.1 pilin Machinery gene
  LOY31_RS24710 (LOY31_24690) pilB 5503247..5504947 (+) 1701 WP_221535412.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LOY31_RS24715 (LOY31_24695) pilC 5504950..5506167 (+) 1218 WP_258712317.1 type II secretion system F family protein Machinery gene
  LOY31_RS24720 (LOY31_24700) pilD 5506169..5507041 (+) 873 WP_258712318.1 A24 family peptidase Machinery gene
  LOY31_RS24725 (LOY31_24705) coaE 5507038..5507661 (+) 624 WP_007959586.1 dephospho-CoA kinase -
  LOY31_RS24730 (LOY31_24710) yacG 5507658..5507858 (+) 201 WP_007959585.1 DNA gyrase inhibitor YacG -
  LOY31_RS24735 (LOY31_24715) - 5507865..5508080 (-) 216 WP_007959584.1 hypothetical protein -
  LOY31_RS24740 (LOY31_24720) - 5508146..5508835 (-) 690 WP_221535409.1 energy-coupling factor ABC transporter permease -
  LOY31_RS24745 (LOY31_24725) - 5508969..5509595 (+) 627 WP_007959580.1 DUF1780 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62804.82 Da        Isoelectric Point: 5.9468

>NTDB_id=627764 LOY31_RS24710 WP_221535412.1 5503247..5504947(+) (pilB) [Pseudomonas sp. B21-021]
MNDIALSGLAKQLVQAELLTEKSAQQAWQQAQRNRLSLVSYLVQNKLVKSWQVAEIASEHFGMAFLDLNCLDKETQPKGL
VSEKLVRQHHALPLWRRGNKLFVGISDPSNHQAINDIQFSTGLSTEAILVEDDKLTDAIEKFFDTHATGLEEMADVDLDG
LDIESVDDSKQDTLGGIDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNYRVRVRTDGILREVAKPPIQLAGRIAARL
KVMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVMRILDPSSAQIGIDALGYEPEQKDLYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCSHCKKPIEIPRETLIKEGFPE
ERIGHFTIYEPSGCDHCNGGYKGRVGIYEVVKNTPELQRLIMAEGNSLEIDTQMRRDGFNDLRTSGLIKAMQGITGLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=627764 LOY31_RS24710 WP_221535412.1 5503247..5504947(+) (pilB) [Pseudomonas sp. B21-021]
ATGAATGACATCGCTTTGAGCGGTCTGGCCAAACAACTGGTCCAGGCTGAATTGCTCACGGAAAAGAGTGCCCAGCAAGC
CTGGCAACAGGCGCAGCGCAATCGCCTGTCGCTGGTCAGTTACCTGGTGCAGAACAAACTGGTGAAGAGCTGGCAGGTCG
CCGAAATAGCCTCGGAGCATTTCGGCATGGCATTTCTCGACCTCAACTGTCTCGACAAGGAAACCCAGCCCAAGGGCCTG
GTCAGTGAAAAACTGGTGCGTCAGCACCACGCCCTGCCCCTCTGGCGGCGCGGCAACAAACTGTTCGTGGGGATTTCCGA
CCCGAGCAATCATCAGGCGATCAATGACATTCAGTTCAGCACCGGGCTGAGCACCGAAGCCATTCTGGTCGAGGACGACA
AACTCACCGACGCCATTGAAAAATTCTTCGACACCCACGCCACCGGCCTGGAGGAGATGGCCGATGTCGACCTCGACGGG
CTGGATATCGAATCGGTCGACGACAGCAAGCAGGACACGCTCGGCGGGATCGATGCGGACGATGCCCCGGTGGTGCGCTT
CGTGCACAAGATGCTGCTGGACGCGATCAAGAGCGGCTCGTCCGACCTGCACTTCGAGCCTTACGAAAAGAATTACCGGG
TGCGGGTGCGCACCGACGGCATTCTGCGGGAAGTGGCCAAGCCGCCGATTCAGCTCGCCGGGCGCATTGCCGCGCGCCTG
AAGGTCATGGCCAGCCTCGACATTTCGGAGCGGCGCAAACCCCAGGACGGGCGGATCAAGATGCGTCTGTCGAAGAGCAA
GTCGATCGATTTCCGGGTCAACACTCTGCCCACGCTCTGGGGCGAGAAAGTGGTGATGCGGATCCTCGACCCGTCCAGCG
CCCAGATCGGCATCGATGCCCTCGGCTACGAGCCGGAGCAGAAAGACCTGTACATGGCCGCCCTCAAGCAGCCGCAAGGC
ATGATTCTGGTGACCGGCCCCACCGGCTCGGGCAAGACCGTGTCGCTGTACACCGGGCTGAACATCCTCAATACCGTCGA
CATCAACATTTCTACCGCCGAAGACCCGGTGGAGATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCACGCCAAG
GGTTGGACTTTGCCCAGGCCCTGCGCTCGTTCCTGCGTCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTC
GAAACCGCCGAAATCGCGATCAAGGCCGCCCAGACCGGGCACCTCGTGTTGTCTACCCTGCACACCAACAGCGCAGCGGA
AACTCTGACCCGCCTGCACAACATGGGCATTCCCGGCTTCAACATCGCGACCTCGGTGAGCCTGATCATTGCCCAGCGCC
TGGCGCGAAAACTCTGCAGCCACTGCAAGAAACCCATCGAAATCCCTCGCGAAACCCTGATCAAGGAAGGTTTCCCCGAG
GAACGCATCGGCCATTTCACGATCTATGAGCCCTCTGGTTGCGATCACTGCAACGGCGGGTACAAGGGTCGCGTGGGGAT
TTATGAAGTGGTGAAGAACACCCCCGAACTGCAACGGCTGATCATGGCCGAAGGCAACTCACTGGAAATCGATACCCAGA
TGCGCCGGGACGGCTTCAACGACCTGCGCACCTCGGGCCTGATCAAGGCCATGCAAGGCATCACCGGCCTTGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.025

100

0.581
  pilB Acinetobacter baumannii D1279779

57.672

100

0.578
  pilB Legionella pneumophila strain ERS1305867

56.36

100

0.564
  pilB Vibrio cholerae strain A1552

50.619

99.823

0.505
  pilB Vibrio parahaemolyticus RIMD 2210633

49.206

100

0.493
  pilB Vibrio campbellii strain DS40M4

48.264

100

0.491
  pilF Neisseria gonorrhoeae MS11

49.023

99.47

0.488
  pilF Thermus thermophilus HB27

37.097

98.587

0.366
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.826

93.286

0.362