Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LL022_RS18705 Genome accession   NZ_CP086101
Coordinates   3940676..3941701 (-) Length   341 a.a.
NCBI ID   WP_228176447.1    Uniprot ID   -
Organism   Enterobacter ludwigii strain 994590     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3935676..3946701
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LL022_RS18675 (LL022_18720) - 3936577..3937074 (+) 498 WP_032670169.1 SprT family zinc-dependent metalloprotease -
  LL022_RS18680 (LL022_18725) endA 3937169..3937876 (+) 708 WP_020883698.1 deoxyribonuclease I -
  LL022_RS18685 (LL022_18730) rsmE 3937929..3938660 (+) 732 WP_020883697.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  LL022_RS18690 (LL022_18735) gshB 3938700..3939647 (+) 948 WP_020883696.1 glutathione synthase -
  LL022_RS18695 (LL022_18740) - 3939734..3940294 (+) 561 WP_020883695.1 YqgE/AlgH family protein -
  LL022_RS18700 (LL022_18745) ruvX 3940294..3940710 (+) 417 WP_014171567.1 Holliday junction resolvase RuvX -
  LL022_RS18705 (LL022_18750) pilT 3940676..3941701 (-) 1026 WP_228176447.1 type IV pilus twitching motility protein PilT Machinery gene
  LL022_RS18710 (LL022_18755) - 3941719..3942420 (+) 702 WP_228176446.1 YggS family pyridoxal phosphate-dependent enzyme -
  LL022_RS18715 (LL022_18760) - 3942442..3943008 (+) 567 WP_014171570.1 YggT family protein -
  LL022_RS18720 (LL022_18765) yggU 3943005..3943301 (+) 297 WP_014885193.1 DUF167 family protein YggU -
  LL022_RS18725 (LL022_18770) - 3943305..3943898 (+) 594 WP_014171572.1 XTP/dITP diphosphatase -
  LL022_RS18730 (LL022_18775) hemW 3943891..3945033 (+) 1143 WP_228176445.1 radical SAM family heme chaperone HemW -
  LL022_RS18735 (LL022_18780) - 3945097..3945459 (+) 363 WP_228176444.1 endonuclease domain-containing protein -
  LL022_RS18740 (LL022_18785) - 3945521..3946237 (-) 717 WP_040018846.1 DUF2884 domain-containing protein -
  LL022_RS18745 (LL022_18790) - 3946294..3946620 (-) 327 WP_032678951.1 YggL family protein -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 37120.13 Da        Isoelectric Point: 5.8653

>NTDB_id=622522 LL022_RS18705 WP_228176447.1 3940676..3941701(-) (pilT) [Enterobacter ludwigii strain 994590]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPEVDTLLKAWLNDEQQGTWWANGQVDFAVTLAGNQRLRG
SAFKETSGVSMTLRLLPGVCPQLSALGVPRVVPELLSSDNGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
FLYQSERCLIQQREIGQHSPSFADALRGALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLLPDAQGGRVALYELLVNTPAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGQLQAPALNNPVRNSFRE

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=622522 LL022_RS18705 WP_228176447.1 3940676..3941701(-) (pilT) [Enterobacter ludwigii strain 994590]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAATGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGATCAGGTCGTCTTGAACCCGCGCCGTTTCCGCCTCCCGAAGTCGATACGCTATTAAAGGCGTGGCTCAACG
ATGAACAGCAGGGAACGTGGTGGGCGAACGGGCAGGTGGATTTTGCCGTCACCCTGGCGGGCAATCAGCGCCTGCGCGGA
AGCGCGTTTAAAGAGACAAGCGGCGTCTCAATGACGCTGCGGCTGTTGCCCGGAGTCTGCCCGCAACTCTCCGCGTTGGG
GGTGCCCCGGGTTGTTCCTGAGCTGTTATCCAGTGACAACGGGCTAATTCTGGTCACCGGGGCAACCGGCAGCGGTAAGT
CCACTACGCTGGCAGCGATGGTGGATTTTCTTAATCACCATACTGACGGGCATATTCTGACGCTCGAAGACCCGGTGGAG
TTTCTGTACCAGAGCGAACGTTGCCTGATCCAGCAGCGCGAGATTGGCCAACACAGCCCGTCATTTGCCGACGCGCTGCG
CGGGGCGTTACGCCAGGATCCTGATGTCATACTGCTGGGAGAGCTGCGCGACAGTGAAACAATCCGCCTCGCGCTGACGG
CGGCAGAAACCGGACATCTGGTGCTCGCGACACTGCATACTCGCGGGGCAGCGCAGGCGATTGAGCGGCTGGTCGATACG
TTTCCGGCGCAGGAGAAAGATCCGGTGCGCAATCAGCTGGCCGGCAGCCTGCGGGCGGTGCTGGCGCAAAAGCTCCTCCC
CGATGCGCAGGGCGGGCGCGTGGCGCTTTATGAGCTGCTGGTGAATACTCCGGCGGCGGCGAATCTCATTCGCGAAGGAA
AAACGTGGCAATTGCCCGGTATTATTCAAACCGGCCAGCAGGCGGGGATGCAGAACTTTGACCAGAGCCTGGCTGAAAGA
CGGGCGCAGGGGCAACTCCAGGCCCCGGCGCTCAATAACCCTGTTCGAAATAGCTTTCGAGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.817

99.12

0.484
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.817

99.12

0.484
  pilT Neisseria meningitidis 8013

48.348

97.654

0.472
  pilT Neisseria gonorrhoeae MS11

46.784

100

0.469
  pilT Legionella pneumophila strain Lp02

48.758

94.428

0.46
  pilT Legionella pneumophila strain ERS1305867

48.758

94.428

0.46
  pilT Pseudomonas stutzeri DSM 10701

47.401

95.894

0.455
  pilT Acinetobacter baylyi ADP1

46.687

97.361

0.455
  pilT Acinetobacter baumannii D1279779

47.095

95.894

0.452
  pilT Acinetobacter baumannii strain A118

47.095

95.894

0.452
  pilT Pseudomonas aeruginosa PAK

47.095

95.894

0.452
  pilT Acinetobacter nosocomialis M2

47.5

93.842

0.446
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.41

94.428

0.419
  pilU Pseudomonas stutzeri DSM 10701

40

96.774

0.387
  pilU Vibrio cholerae strain A1552

39.13

94.428

0.369