Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PCC6301pg_RS10690 Genome accession   NZ_CP085785
Coordinates   2145851..2146939 (-) Length   362 a.a.
NCBI ID   WP_011378300.1    Uniprot ID   Q31LG9
Organism   Synechococcus elongatus PCC 6301     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2140851..2151939
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCC6301pg_RS10665 (PCC6301pg_2145) - 2141413..2141667 (-) 255 WP_011378297.1 helix-hairpin-helix domain-containing protein -
  PCC6301pg_RS10670 (PCC6301pg_2146) dusA 2141764..2142795 (-) 1032 WP_011244337.1 tRNA dihydrouridine(20/20a) synthase DusA -
  PCC6301pg_RS10675 (PCC6301pg_2147) - 2142822..2144069 (+) 1248 WP_011378298.1 NAD(P)/FAD-dependent oxidoreductase -
  PCC6301pg_RS10680 (PCC6301pg_2148) - 2144034..2144588 (-) 555 WP_011244335.1 hypothetical protein -
  PCC6301pg_RS10685 (PCC6301pg_2149) - 2144594..2145826 (-) 1233 WP_011378299.1 type II secretion system F family protein -
  PCC6301pg_RS10690 (PCC6301pg_2150) pilT 2145851..2146939 (-) 1089 WP_011378300.1 type IV pilus twitching motility protein PilT Machinery gene
  PCC6301pg_RS10695 (PCC6301pg_2151) pilB/pilB1 2147010..2149010 (-) 2001 WP_011378301.1 GspE/PulE family protein Machinery gene
  PCC6301pg_RS10700 (PCC6301pg_2152) grpE 2149287..2149910 (+) 624 WP_011378302.1 nucleotide exchange factor GrpE -

Sequence

Protein


Download         Length: 362 a.a.        Molecular weight: 39881.02 Da        Isoelectric Point: 6.3643

>NTDB_id=619914 PCC6301pg_RS10690 WP_011378300.1 2145851..2146939(-) (pilT) [Synechococcus elongatus PCC 6301]
MELMIEDLMEKLVEMGGSDLHLSVGLPPYFRISGKLTPIGDEPMGEEQCQRLIFSMLNNTQRKMLEQNWELDCSYGIRGL
ARFRVNVYKDKGTYAACLRALSAKVPEFNDLGLPQVVREMSEKPRGLILVTGPTGSGKTTTLAAVIELINRSRAEHIITV
EDPIEFVYQPAKSIIHQRQLGEDTKSFANALRAALREDPDIILVGEMRDLETIQLAITAAETGHLVFGTLHTSSAAQTVD
RIIDVFPAEKQTQVRVQLSNSLIAVFSQTLVQKRNARPGSFGRVLAQEIMIVTPAISNLIREGKTAQIYSQIQTGGKLGM
QTLEKALADLYKAGEITFEAAASKTSKPDEMQRLIGNVPVAA

Nucleotide


Download         Length: 1089 bp        

>NTDB_id=619914 PCC6301pg_RS10690 WP_011378300.1 2145851..2146939(-) (pilT) [Synechococcus elongatus PCC 6301]
ATGGAGCTGATGATTGAAGACCTGATGGAAAAGCTGGTGGAGATGGGTGGATCTGACCTTCACCTTTCCGTTGGTCTGCC
GCCCTATTTTCGAATTAGTGGCAAACTGACACCGATCGGCGATGAGCCGATGGGCGAAGAACAGTGCCAACGCCTGATTT
TCAGCATGTTGAACAACACGCAACGCAAAATGCTGGAGCAGAACTGGGAGTTAGACTGCTCCTACGGCATCCGTGGTCTA
GCTCGGTTTCGGGTCAACGTCTATAAAGACAAGGGAACCTATGCGGCCTGTTTGCGGGCACTCAGTGCCAAGGTCCCAGA
GTTTAACGATCTCGGCCTGCCACAGGTTGTCCGGGAAATGAGCGAGAAACCGCGAGGGCTGATCCTAGTGACAGGGCCCA
CGGGTTCAGGCAAGACCACAACTTTGGCTGCAGTGATTGAACTAATCAATCGCAGCCGTGCTGAACATATCATCACGGTT
GAAGACCCAATTGAGTTTGTCTATCAACCGGCAAAAAGTATCATCCACCAGCGTCAGCTGGGTGAGGATACCAAGAGTTT
TGCCAACGCGCTGCGAGCTGCCCTGCGGGAAGACCCTGACATTATCCTCGTGGGTGAGATGCGGGACTTGGAAACGATCC
AGTTGGCAATCACTGCTGCAGAGACCGGCCACCTTGTCTTTGGTACCTTACACACCAGCTCTGCAGCCCAAACCGTTGAC
CGGATTATTGACGTTTTCCCGGCTGAAAAACAGACACAGGTGCGGGTGCAACTCTCGAACTCTCTGATTGCAGTTTTTAG
TCAGACCCTAGTACAGAAACGCAATGCTCGTCCGGGCAGTTTTGGCCGAGTCTTGGCTCAGGAAATTATGATTGTGACGC
CGGCAATCTCGAACTTGATTCGGGAAGGGAAAACGGCGCAGATCTATTCCCAAATTCAGACGGGTGGCAAGTTGGGAATG
CAGACGCTCGAAAAAGCGCTGGCAGACCTCTACAAAGCAGGTGAAATTACCTTTGAAGCAGCTGCATCCAAAACGTCGAA
GCCTGACGAGATGCAACGACTCATCGGTAATGTTCCGGTTGCTGCATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(48-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q31LG9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

53.314

95.856

0.511
  pilT Legionella pneumophila strain ERS1305867

53.623

95.304

0.511
  pilT Legionella pneumophila strain Lp02

53.623

95.304

0.511
  pilT Pseudomonas aeruginosa PAK

53.026

95.856

0.508
  pilT Acinetobacter baylyi ADP1

53.333

95.304

0.508
  pilT Acinetobacter baumannii strain A118

52.464

95.304

0.5
  pilT Acinetobacter nosocomialis M2

52.464

95.304

0.5
  pilT Acinetobacter baumannii D1279779

52.464

95.304

0.5
  pilT Neisseria gonorrhoeae MS11

49.857

96.409

0.481
  pilT Neisseria meningitidis 8013

49.857

96.409

0.481
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.857

96.685

0.472
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.412

93.923

0.464
  pilT Vibrio cholerae strain A1552

49.412

93.923

0.464
  pilU Pseudomonas stutzeri DSM 10701

39.601

96.961

0.384