Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   PCC6301pg_RS10695 Genome accession   NZ_CP085785
Coordinates   2147010..2149010 (-) Length   666 a.a.
NCBI ID   WP_011378301.1    Uniprot ID   -
Organism   Synechococcus elongatus PCC 6301     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2142010..2154010
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCC6301pg_RS10675 (PCC6301pg_2147) - 2142822..2144069 (+) 1248 WP_011378298.1 NAD(P)/FAD-dependent oxidoreductase -
  PCC6301pg_RS10680 (PCC6301pg_2148) - 2144034..2144588 (-) 555 WP_011244335.1 hypothetical protein -
  PCC6301pg_RS10685 (PCC6301pg_2149) - 2144594..2145826 (-) 1233 WP_011378299.1 type II secretion system F family protein -
  PCC6301pg_RS10690 (PCC6301pg_2150) pilT 2145851..2146939 (-) 1089 WP_011378300.1 type IV pilus twitching motility protein PilT Machinery gene
  PCC6301pg_RS10695 (PCC6301pg_2151) pilB/pilB1 2147010..2149010 (-) 2001 WP_011378301.1 GspE/PulE family protein Machinery gene
  PCC6301pg_RS10700 (PCC6301pg_2152) grpE 2149287..2149910 (+) 624 WP_011378302.1 nucleotide exchange factor GrpE -
  PCC6301pg_RS10705 (PCC6301pg_2153) dnaK 2150015..2151982 (+) 1968 WP_039755608.1 molecular chaperone DnaK -
  PCC6301pg_RS10710 (PCC6301pg_2154) dnaJ 2152124..2153254 (+) 1131 WP_011244329.1 molecular chaperone DnaJ -
  PCC6301pg_RS10715 (PCC6301pg_2155) - 2153251..2153502 (+) 252 WP_011244328.1 sulfurtransferase TusA family protein -

Sequence

Protein


Download         Length: 666 a.a.        Molecular weight: 74230.85 Da        Isoelectric Point: 5.0768

>NTDB_id=619915 PCC6301pg_RS10695 WP_011378301.1 2147010..2149010(-) (pilB/pilB1) [Synechococcus elongatus PCC 6301]
MPDQPISPRYALIALNQFSSFGNKLVQAGFVDPEQLQTALVESRRSGQSLPQVISALTGQSLPPELMQQYRRQQLFELKI
LHGVESLNPDQGQYSEEVIHELIADLVPIETCRRHQLVPLERHEAPPRAVVAMVDPNNLDALDELNRLLRPRGYTLQRLV
ITLEDYERLIAGYLDQQLERQQAMQAKQLGDFSEELANLDNLELTEISVQEEDLAKALQEADDAPIVSLANKILLKALTE
GASDIHVEPQEDGLRVRLRRDGVLHQEFGTLPKKIQPALISRLKIMANLDIAERRVPQDGRIRRMFQGRKIDFRVNSLPS
HFGEKICMRLLDNSATQLGLDQLITDEEALALVRDMGSRPYGLILVTGPTGSGKSTSLYSLLAERNDPAININTAEDPIE
YTLPGLTQVQVLREKGMNFASILRAFMRQDPDVILVGETRDLETARTAIEAALTGHLVLTTLHANDAPSAIARLSEMGVE
PYLVSGSLIGVVSQRLMRRVCPDCRVPYTPNPEELGRYGLSASQELDVTFYKAKTLTSDSLAQARSQGQVCRTCRGTGYK
GRVGVYEVMRVNEVLQVAINQGLPTERLKEIAVEQGMKTLLSYSLELVRQGYTTFEEVDRVTFTDSGVEAELKAKRKLGL
TCNTCGAGLQPEWLDCPYCLTPRFGN

Nucleotide


Download         Length: 2001 bp        

>NTDB_id=619915 PCC6301pg_RS10695 WP_011378301.1 2147010..2149010(-) (pilB/pilB1) [Synechococcus elongatus PCC 6301]
ATGCCAGACCAACCGATCTCTCCCCGTTACGCCCTGATTGCCCTCAATCAGTTTTCATCGTTTGGTAACAAGCTGGTTCA
AGCGGGCTTTGTCGATCCCGAGCAACTACAGACAGCCCTGGTGGAAAGTCGCCGCAGCGGCCAGTCGCTGCCTCAAGTGA
TCTCAGCCCTCACTGGCCAGTCTTTGCCCCCCGAACTGATGCAGCAGTATCGGCGGCAGCAGTTGTTTGAACTCAAAATT
CTGCACGGGGTCGAGTCGCTTAACCCAGACCAGGGGCAGTACTCCGAAGAGGTCATCCACGAGCTGATCGCTGACTTAGT
GCCGATCGAAACCTGTCGCCGTCATCAACTCGTCCCCCTCGAACGCCATGAAGCGCCGCCGCGAGCCGTGGTCGCTATGG
TCGACCCCAATAACCTCGATGCCTTGGACGAACTCAACCGGCTACTGCGACCCAGGGGCTACACCCTACAGCGGCTCGTG
ATTACCCTCGAGGATTACGAACGGCTGATTGCGGGCTATCTGGATCAGCAGCTGGAACGTCAGCAGGCTATGCAAGCCAA
GCAGCTGGGGGATTTTTCCGAGGAGCTTGCGAACCTCGACAACTTGGAACTGACGGAAATTTCAGTCCAAGAAGAGGATC
TTGCCAAGGCGTTGCAAGAGGCCGATGATGCGCCGATCGTGAGTCTCGCTAACAAAATCCTGCTGAAGGCTTTGACGGAA
GGCGCTTCCGATATCCACGTCGAGCCCCAGGAAGACGGGCTACGAGTGCGACTGCGACGAGATGGAGTCTTGCACCAAGA
ATTTGGGACGCTTCCCAAAAAAATTCAACCCGCCCTGATCTCTCGGCTAAAGATCATGGCCAATCTGGACATCGCCGAGC
GGCGGGTCCCTCAAGATGGCCGCATTCGACGCATGTTCCAAGGCCGCAAGATCGACTTCCGGGTCAACAGCCTCCCTAGT
CACTTCGGTGAAAAGATTTGTATGCGGTTGCTGGACAACTCAGCAACCCAACTGGGTTTAGACCAGTTGATTACTGATGA
AGAAGCGTTGGCCTTGGTGCGTGACATGGGTAGTCGCCCCTATGGTCTCATTCTCGTAACAGGCCCTACGGGGTCTGGAA
AGTCGACGAGCCTGTACTCGCTGCTGGCGGAGCGCAATGATCCGGCGATCAATATCAACACCGCTGAAGACCCGATCGAG
TACACCTTGCCCGGGCTGACCCAAGTGCAGGTTCTCCGGGAGAAAGGCATGAACTTTGCCTCAATTCTCCGGGCCTTCAT
GCGGCAGGATCCCGATGTGATTCTGGTGGGTGAAACGCGCGATTTGGAAACGGCCCGCACGGCGATTGAAGCGGCCTTGA
CCGGCCACTTAGTCTTGACAACCCTGCATGCGAACGATGCTCCGAGTGCGATCGCGAGGCTGAGTGAAATGGGAGTGGAG
CCCTACCTAGTCTCGGGGTCACTGATTGGGGTGGTCTCGCAACGTTTGATGCGCCGCGTCTGCCCCGACTGTCGTGTTCC
CTACACCCCCAACCCAGAAGAACTAGGTCGCTATGGACTGTCGGCGAGTCAGGAGCTGGATGTCACCTTTTATAAGGCCA
AAACATTGACGTCGGATTCGCTGGCTCAGGCGCGATCGCAGGGACAGGTTTGCAGAACTTGTCGTGGGACTGGCTACAAG
GGCCGCGTCGGGGTTTATGAGGTGATGCGGGTCAATGAAGTGCTTCAGGTGGCGATCAACCAAGGACTCCCGACTGAACG
CCTCAAGGAAATTGCGGTGGAGCAGGGGATGAAAACCTTACTCTCCTACAGCTTGGAGTTGGTCCGTCAGGGATACACCA
CCTTTGAGGAAGTTGACCGGGTGACCTTTACCGATTCTGGCGTGGAGGCCGAACTCAAGGCCAAGCGCAAACTAGGCCTG
ACCTGCAACACCTGTGGGGCGGGTCTGCAACCCGAATGGCTGGACTGCCCCTATTGTCTGACGCCCCGCTTTGGGAACTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(232-499)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(25-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

61.002

98.949

0.604