Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LJU47_RS17110 Genome accession   NZ_CP085743
Coordinates   3527006..3527986 (+) Length   326 a.a.
NCBI ID   WP_060580281.1    Uniprot ID   -
Organism   Enterobacter hormaechei strain 60214CZ     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3522006..3532986
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LJU47_RS17070 (LJU47_17070) - 3522082..3522408 (+) 327 WP_003862421.1 YggL family protein -
  LJU47_RS17075 (LJU47_17075) - 3522466..3523182 (+) 717 WP_003862419.1 DUF2884 domain-containing protein -
  LJU47_RS17080 (LJU47_17080) - 3523249..3523611 (-) 363 WP_045337264.1 DUF559 domain-containing protein -
  LJU47_RS17085 (LJU47_17085) hemW 3523668..3524816 (-) 1149 WP_003860019.1 radical SAM family heme chaperone HemW -
  LJU47_RS17090 (LJU47_17090) - 3524809..3525402 (-) 594 WP_022651831.1 XTP/dITP diphosphatase -
  LJU47_RS17095 (LJU47_17095) yggU 3525406..3525702 (-) 297 WP_003860023.1 DUF167 family protein YggU -
  LJU47_RS17100 (LJU47_17100) - 3525699..3526265 (-) 567 WP_003860024.1 YggT family protein -
  LJU47_RS17105 (LJU47_17105) - 3526287..3526988 (-) 702 WP_032610102.1 YggS family pyridoxal phosphate-dependent enzyme -
  LJU47_RS17110 (LJU47_17110) pilT 3527006..3527986 (+) 981 WP_060580281.1 type IV pilus twitching motility protein PilT Machinery gene
  LJU47_RS17115 (LJU47_17115) ruvX 3527997..3528413 (-) 417 WP_003860030.1 Holliday junction resolvase RuvX -
  LJU47_RS17120 (LJU47_17120) - 3528413..3528973 (-) 561 WP_003860031.1 YqgE/AlgH family protein -
  LJU47_RS17125 (LJU47_17125) gshB 3529048..3529995 (-) 948 WP_023323729.1 glutathione synthase -
  LJU47_RS17130 (LJU47_17130) rsmE 3530023..3530754 (-) 732 WP_045345616.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  LJU47_RS17135 (LJU47_17135) endA 3530806..3531513 (-) 708 WP_003860034.1 deoxyribonuclease I -
  LJU47_RS17140 (LJU47_17140) - 3531608..3532105 (-) 498 WP_003860035.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35670.69 Da        Isoelectric Point: 6.4125

>NTDB_id=619745 LJU47_RS17110 WP_060580281.1 3527006..3527986(+) (pilT) [Enterobacter hormaechei strain 60214CZ]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVEALLKAWLNDEQQGAWWANGQVDFAVTLTGGQRLRG
SAFKHMHGVSIALRLLPLTCPQLSALGVPRAIPELLSNDNGLILVTGATGSGKSTTLAAMVYFLNHQTDGHILTLEDPVE
FMYQSERCLIQQREIGLHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLRQDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=619745 LJU47_RS17110 WP_060580281.1 3527006..3527986(+) (pilT) [Enterobacter hormaechei strain 60214CZ]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCACCACCGCG
CTGGCGCAGGTCAGGCCGTCTTGAACCTGCGCCGTTTCCGCCCCCGGATGTGGAGGCGTTATTAAAAGCGTGGCTCAATG
ACGAACAGCAGGGCGCCTGGTGGGCAAACGGGCAGGTGGATTTTGCCGTTACCCTCACAGGCGGTCAGCGGCTGCGCGGC
AGTGCGTTTAAGCATATGCACGGCGTTTCGATCGCGCTGCGCCTGTTGCCGCTGACGTGCCCGCAGCTCTCTGCGTTAGG
CGTGCCGCGCGCGATCCCGGAGCTTTTGTCCAATGACAATGGCCTGATTCTGGTCACCGGCGCCACCGGCAGCGGGAAAT
CGACCACTCTGGCCGCGATGGTCTATTTTCTCAATCACCAGACGGACGGGCACATTCTGACCCTTGAAGATCCGGTGGAG
TTTATGTACCAGAGTGAACGTTGCCTGATCCAGCAGCGGGAGATAGGCCTGCACAGCCCGTCCTTTGCCGAGGCCCTGCG
CAGCGCGCTGCGTGAAGATCCGGATGTAATTCTGCTTGGTGAGCTGCGCGACAGCGAGACGATACGCCTGGCGCTGACGG
CGGCGGAAACCGGCCATCTGGTGTTGGCCACGCTGCACACGCGCGGTGCGGCGCAGGCGATCGAACGCCTGGTCGACACC
TTTCCGGCGCAGGAGAAAGATCCGGTGCGTAATCAGCTGGCGGGAAGCCTGCGTGCGGTTCTGGCGCAGAAGCTGCGTCA
GGATGTCCAGGGCGGGCGCGTGGCGCTGTATGAGCTACTGGTGAATACGTCGGCGGCGGCAAACCTGATCCGCGAAGGCA
AAACCTGGCAACTGCCGGGCATTATTCAAACGGGGCAGCAGGCGGGGATGCAGAACTTTGAGCAGAGTCTGGCGGAGCGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(75-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Pseudomonas stutzeri DSM 10701

47.706

100

0.479
  pilT Legionella pneumophila strain Lp02

48.137

98.773

0.475
  pilT Legionella pneumophila strain ERS1305867

48.137

98.773

0.475
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Acinetobacter baumannii strain A118

46.789

100

0.469
  pilT Pseudomonas aeruginosa PAK

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.789

100

0.469
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

47.188

98.16

0.463
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.034

99.08

0.426
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.387
  pilU Vibrio cholerae strain A1552

38.75

98.16

0.38