Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LG320_RS11765 Genome accession   NZ_CP085018
Coordinates   2213406..2214449 (-) Length   347 a.a.
NCBI ID   WP_026832417.1    Uniprot ID   -
Organism   Exiguobacterium undae strain SLM-182     
Function   pseudopilus biogenesis (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2208406..2219449
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LG320_RS11745 (LG320_11730) - 2208951..2210693 (-) 1743 WP_404274545.1 fimbrial assembly protein -
  LG320_RS11750 (LG320_11735) - 2210712..2211467 (-) 756 WP_026832420.1 A24 family peptidase -
  LG320_RS11755 (LG320_11740) - 2211527..2212045 (-) 519 WP_404274547.1 type II secretion system protein -
  LG320_RS11760 (LG320_11745) - 2212184..2213401 (-) 1218 WP_119923267.1 type II secretion system F family protein -
  LG320_RS11765 (LG320_11750) pilT 2213406..2214449 (-) 1044 WP_026832417.1 type IV pilus twitching motility protein PilT Machinery gene
  LG320_RS11770 (LG320_11755) - 2214446..2216110 (-) 1665 WP_028105914.1 GspE/PulE family protein -
  LG320_RS11775 (LG320_11760) - 2216119..2216433 (-) 315 WP_404274548.1 hypothetical protein -
  LG320_RS11780 (LG320_11765) - 2216460..2217854 (-) 1395 WP_404274549.1 G5 domain-containing protein -
  LG320_RS11785 (LG320_11770) - 2218030..2218554 (+) 525 WP_028105911.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  LG320_RS11790 (LG320_11775) - 2218517..2219131 (+) 615 WP_404274550.1 type II secretion system protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38104.95 Da        Isoelectric Point: 7.4701

>NTDB_id=614574 LG320_RS11765 WP_026832417.1 2213406..2214449(-) (pilT) [Exiguobacterium undae strain SLM-182]
MSTMERAQIEDILRSSKEKGASDVHLTAGSPPVFRVNGTLMPFGTDKMKPIIIDGFVRSLITEEMYLQLKQDRDIDFSFG
VPGVARYRVNCFYQRGALSMAFRTIPTEVPTLEQLSLPPVLKRFLTKPHGLILVTGPTGSGKSTTLAAMINEINQTMYKR
IITLEDPIEYLHSHQKSLIDQREIGIDAPRFATGLRSALRQDPDVILLGEMRDLETISTAVTAAETGHLVFATVHTSTAA
STVSRIIDVFPSEQQDQIRAQLANVLAGVVSQRLMPRTDGTGRVAALEIMVETPAVSNLIRTGKEYQIQSVLQTGKQYGM
QTMQMALQDLVSRGLIPASAATPYISG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=614574 LG320_RS11765 WP_026832417.1 2213406..2214449(-) (pilT) [Exiguobacterium undae strain SLM-182]
ATGAGTACGATGGAACGCGCACAAATCGAAGACATCCTACGGTCATCAAAAGAAAAGGGCGCATCTGACGTCCATTTGAC
AGCGGGATCACCGCCTGTCTTTCGTGTCAACGGCACATTGATGCCGTTTGGAACGGACAAGATGAAGCCGATCATCATCG
ACGGATTCGTCCGTTCACTGATTACCGAGGAAATGTATCTCCAACTCAAACAAGACCGCGACATCGATTTTTCGTTTGGT
GTGCCGGGAGTCGCCCGCTACCGCGTCAACTGTTTTTATCAGCGCGGAGCGCTGTCGATGGCGTTTCGGACGATTCCGAC
GGAGGTGCCGACACTCGAACAACTGTCGTTACCACCGGTCTTAAAACGTTTCCTGACAAAGCCCCACGGATTGATTCTCG
TGACCGGTCCGACCGGTTCCGGGAAATCAACGACCTTGGCTGCAATGATCAACGAGATCAACCAGACGATGTACAAACGG
ATCATCACGCTTGAAGATCCAATCGAGTATTTGCACAGCCACCAAAAAAGTTTGATCGATCAACGGGAAATCGGAATTGA
TGCCCCGCGGTTTGCGACCGGTTTACGCTCGGCGCTCCGGCAGGATCCGGACGTCATCTTACTCGGTGAGATGCGGGATC
TGGAGACGATTTCGACTGCCGTCACGGCGGCTGAGACCGGGCATTTAGTGTTTGCGACCGTCCATACCTCGACGGCGGCT
TCGACCGTCAGTCGGATCATTGACGTCTTCCCGTCGGAACAACAGGATCAAATCCGGGCGCAACTGGCGAACGTTTTGGC
CGGCGTCGTCTCGCAACGGCTGATGCCGCGGACGGACGGGACAGGACGTGTTGCCGCCCTCGAAATCATGGTCGAGACAC
CGGCTGTTTCAAACCTGATCCGGACCGGCAAAGAATATCAGATTCAATCTGTCCTGCAGACCGGAAAACAATACGGGATG
CAGACGATGCAAATGGCACTACAAGACTTAGTAAGTCGGGGCCTGATCCCAGCATCAGCCGCGACACCTTACATATCGGG
GTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(119-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria gonorrhoeae MS11

50.599

96.254

0.487
  pilT Neisseria meningitidis 8013

50.599

96.254

0.487
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.524

94.524

0.487
  pilT Acinetobacter nosocomialis M2

49.851

96.542

0.481
  pilT Acinetobacter baumannii D1279779

49.552

96.542

0.478
  pilT Acinetobacter baumannii strain A118

49.552

96.542

0.478
  pilT Pseudomonas aeruginosa PAK

49.55

95.965

0.476
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.394

95.101

0.47
  pilT Vibrio cholerae strain A1552

49.394

95.101

0.47
  pilT Pseudomonas stutzeri DSM 10701

48.348

95.965

0.464
  pilT Acinetobacter baylyi ADP1

49.085

94.524

0.464
  pilT Legionella pneumophila strain Lp02

46.547

95.965

0.447
  pilT Legionella pneumophila strain ERS1305867

46.547

95.965

0.447
  pilU Pseudomonas stutzeri DSM 10701

41.337

94.813

0.392
  pilU Acinetobacter baylyi ADP1

39.104

96.542

0.378