Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LG197_RS03170 Genome accession   NZ_CP084714
Coordinates   712552..713550 (-) Length   332 a.a.
NCBI ID   WP_024087855.1    Uniprot ID   V9V7P3
Organism   Pseudomonas asiatica strain P1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 707552..718550
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LG197_RS03150 (LG197_03150) argS 708730..710466 (+) 1737 WP_013974613.1 arginine--tRNA ligase -
  LG197_RS03155 (LG197_03155) - 710468..711166 (+) 699 WP_003257831.1 SPOR domain-containing protein -
  LG197_RS03160 (LG197_03160) - 711203..711622 (-) 420 WP_013974614.1 TM2 domain-containing protein -
  LG197_RS03165 (LG197_03165) - 711789..712424 (+) 636 WP_013974615.1 C40 family peptidase -
  LG197_RS03170 (LG197_03170) pilT 712552..713550 (-) 999 WP_024087855.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LG197_RS03175 (LG197_03175) - 713616..714302 (+) 687 WP_013974617.1 YggS family pyridoxal phosphate-dependent enzyme -
  LG197_RS03180 (LG197_03180) proC 714341..715159 (+) 819 WP_023661482.1 pyrroline-5-carboxylate reductase -
  LG197_RS03185 (LG197_03185) - 715170..715760 (+) 591 WP_013974619.1 YggT family protein -
  LG197_RS03190 (LG197_03190) - 715860..716999 (+) 1140 WP_013974620.1 homoserine O-acetyltransferase -
  LG197_RS03195 (LG197_03195) metW 717007..717627 (+) 621 WP_013974621.1 methionine biosynthesis protein MetW -
  LG197_RS03200 (LG197_03200) - 717650..718084 (+) 435 WP_013974622.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 332 a.a.        Molecular weight: 35899.64 Da        Isoelectric Point: 6.3081

>NTDB_id=612670 LG197_RS03170 WP_024087855.1 712552..713550(-) (pilT) [Pseudomonas asiatica strain P1]
MDVTDLLARAVDAGASDLHLAAGQLPMLRLDGELQRMALPTLGPVALIEGMAPMLDEDQRRQWAQGDELDLALELPALGR
FRLNLFRQLHGPAATFRLIPGHIATLDELDLGDVFQAVAQCRDGLILVGGPTGSGKSSTLATLLDQLNRDRALHIITLED
PVEVIHSSQRSLVNQREVGRHCGGFAQGLRSALRQDPDVIMIGELRDLETIRLALRAAETGHLVLATVHTRSAVSSVDRL
VEVFAAEEKPLVRAMLAELLRLVVAQMLVRRAGGGRVAAREVLVVTPAVRNLVREGRMAQLCSVMQAGGADGMRTMEGAM
RGLKERGLISDL

Nucleotide


Download         Length: 999 bp        

>NTDB_id=612670 LG197_RS03170 WP_024087855.1 712552..713550(-) (pilT) [Pseudomonas asiatica strain P1]
ATGGATGTGACCGACCTGTTGGCCCGGGCCGTGGATGCGGGGGCTTCCGACCTGCACCTGGCGGCGGGCCAGTTACCGAT
GTTGCGCCTGGATGGCGAGCTGCAGCGCATGGCCCTGCCGACCTTGGGCCCGGTCGCCTTGATCGAGGGCATGGCACCCA
TGCTGGATGAAGACCAGCGCCGACAGTGGGCCCAGGGTGATGAGCTGGACCTGGCGCTGGAATTGCCGGCGCTAGGGCGC
TTTCGGCTGAATCTGTTTCGCCAGTTGCACGGCCCTGCGGCCACCTTTCGCCTGATCCCGGGGCATATCGCCACGCTCGA
TGAACTCGACCTGGGCGATGTGTTTCAAGCTGTTGCGCAGTGCCGAGATGGCTTGATCCTCGTCGGTGGGCCGACCGGCA
GTGGCAAGTCCAGCACCTTGGCGACACTGCTCGACCAGCTGAACCGCGATCGTGCTCTGCATATCATCACCCTTGAGGAC
CCTGTCGAAGTTATCCACAGCAGCCAGCGCAGCCTGGTCAACCAGCGTGAGGTCGGCCGCCATTGCGGTGGGTTCGCCCA
GGGGCTGCGCAGTGCCCTGCGCCAAGACCCGGATGTGATCATGATCGGTGAGCTGCGCGACCTGGAAACCATTCGCCTGG
CCTTGCGTGCGGCTGAGACCGGGCACCTGGTACTGGCGACAGTGCATACCCGTTCGGCGGTGAGCAGTGTTGACCGGTTG
GTGGAGGTGTTCGCTGCCGAAGAGAAGCCGTTGGTGCGGGCGATGCTGGCCGAGTTGTTGCGCCTGGTGGTAGCGCAGAT
GCTGGTCAGGCGCGCGGGCGGAGGGCGGGTTGCTGCGCGGGAAGTGCTGGTGGTGACGCCAGCGGTGCGTAACCTGGTTC
GGGAAGGGCGGATGGCGCAGTTGTGTTCGGTGATGCAGGCGGGTGGTGCGGACGGGATGCGGACGATGGAAGGGGCGATG
CGGGGGTTGAAGGAGAGGGGGCTGATCAGCGATCTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB V9V7P3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

51.818

99.398

0.515
  pilT Acinetobacter nosocomialis M2

50.909

99.398

0.506
  pilT Acinetobacter baumannii D1279779

50.909

99.398

0.506
  pilT Acinetobacter baumannii strain A118

50.909

99.398

0.506
  pilT Pseudomonas stutzeri DSM 10701

50.606

99.398

0.503
  pilT Acinetobacter baylyi ADP1

50.303

99.398

0.5
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.936

99.096

0.485
  pilT Vibrio cholerae strain A1552

48.936

99.096

0.485
  pilT Legionella pneumophila strain Lp02

48.485

99.398

0.482
  pilT Legionella pneumophila strain ERS1305867

48.485

99.398

0.482
  pilT Neisseria meningitidis 8013

48.338

99.699

0.482
  pilT Neisseria gonorrhoeae MS11

48.036

99.699

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.921

99.699

0.458
  pilU Pseudomonas stutzeri DSM 10701

37.16

99.699

0.37
  pilU Vibrio cholerae strain A1552

36.81

98.193

0.361