Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   Dongsha4_RS15185 Genome accession   NZ_CP084098
Coordinates   3535885..3536997 (+) Length   370 a.a.
NCBI ID   WP_330203158.1    Uniprot ID   -
Organism   Cyanobacterium sp. Dongsha4     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3530885..3541997
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Dongsha4_RS15155 (Dongsha4_15140) - 3532045..3532242 (-) 198 WP_330203154.1 hypothetical protein -
  Dongsha4_RS15160 (Dongsha4_15145) - 3532332..3533423 (+) 1092 WP_330203155.1 hypothetical protein -
  Dongsha4_RS15165 (Dongsha4_15150) - 3533507..3533830 (+) 324 WP_041922431.1 DUF3593 domain-containing protein -
  Dongsha4_RS15170 (Dongsha4_15155) - 3534109..3534936 (-) 828 WP_330203156.1 urease accessory protein UreD -
  Dongsha4_RS15175 (Dongsha4_15160) - 3535145..3535609 (-) 465 WP_330203157.1 CYTH domain-containing protein -
  Dongsha4_RS15185 (Dongsha4_15170) pilT 3535885..3536997 (+) 1113 WP_330203158.1 type IV pilus twitching motility protein PilT Machinery gene
  Dongsha4_RS15190 (Dongsha4_15175) - 3537029..3538243 (+) 1215 WP_330203159.1 type II secretion system F family protein -
  Dongsha4_RS15195 (Dongsha4_15180) rplU 3538434..3538817 (+) 384 WP_330203160.1 50S ribosomal protein L21 -
  Dongsha4_RS15200 (Dongsha4_15185) rpmA 3538883..3539137 (+) 255 WP_099434755.1 50S ribosomal protein L27 -
  Dongsha4_RS15205 (Dongsha4_15190) - 3539295..3540023 (-) 729 WP_330203161.1 Bax inhibitor-1 family protein -
  Dongsha4_RS15210 (Dongsha4_15195) - 3540054..3540962 (-) 909 WP_330203162.1 PhzF family phenazine biosynthesis protein -
  Dongsha4_RS15215 (Dongsha4_15200) - 3541174..3541749 (+) 576 WP_330203163.1 Uma2 family endonuclease -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40522.86 Da        Isoelectric Point: 6.6491

>NTDB_id=609528 Dongsha4_RS15185 WP_330203158.1 3535885..3536997(+) (pilT) [Cyanobacterium sp. Dongsha4]
MEIMIEDLMEQLVELGGSDMHIQAGAPVYFRISGKLTPIGDEPLNAQECQKLIFSMLNNTQRKTLEQEWELDCSYGVKGL
ARFRVNVYKERGCYAACLRALSSKIPNFDQLGLPDIVKEMSERPRGMILVTGQTGSGKTTTLAAMLDLINRTRAEHILTV
EDPIEYVFPNINSLFHQRQKGEDTKSFANALKAALREDPDIILVGEMRDLETISLAITAAETGHLVFGTLHTSSAAGTID
RIIDVFPSAEQAQIRAMLSNSLLAVFAQTLAKKKNPKPGEFGRAMAQEIMIVTPAIANLIREAKAPQIYSAIQTGGKLGM
QTMEQALANLVKTGTIAVEEALAKSSKPDELKRLLGSSMDSGMVTPGKRR

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=609528 Dongsha4_RS15185 WP_330203158.1 3535885..3536997(+) (pilT) [Cyanobacterium sp. Dongsha4]
ATGGAGATAATGATTGAAGATTTGATGGAGCAGTTAGTAGAATTGGGGGGGTCAGATATGCACATTCAGGCAGGTGCCCC
TGTCTATTTTCGTATTAGCGGTAAACTAACCCCCATCGGAGATGAACCCCTTAATGCTCAAGAGTGCCAAAAATTAATTT
TTAGTATGCTCAATAATACTCAACGTAAAACCTTAGAGCAAGAATGGGAGTTAGACTGTTCTTATGGGGTAAAAGGCTTG
GCTCGATTTCGTGTGAATGTTTATAAGGAGAGGGGTTGTTATGCCGCTTGTTTAAGGGCTTTATCTTCCAAAATTCCCAA
CTTTGATCAATTAGGTTTACCAGATATTGTCAAGGAGATGTCCGAACGCCCTAGAGGTATGATTTTAGTAACGGGGCAGA
CAGGTTCTGGAAAAACCACCACTTTGGCGGCAATGTTGGACTTAATTAACCGCACTCGTGCAGAGCATATCTTAACAGTA
GAAGATCCGATCGAGTATGTTTTTCCTAATATTAACAGTCTATTTCACCAACGTCAAAAAGGAGAAGATACCAAGAGTTT
TGCCAATGCCTTAAAAGCGGCATTGAGGGAAGACCCAGATATTATCCTGGTGGGTGAAATGCGTGACTTAGAAACCATTT
CCTTGGCGATTACTGCGGCGGAAACTGGACACCTTGTCTTTGGTACTTTACATACTAGCTCTGCGGCAGGAACGATTGAC
CGTATTATTGATGTATTTCCTTCTGCGGAACAAGCTCAAATTAGAGCAATGTTATCTAACTCTTTATTGGCAGTATTCGC
TCAAACTTTGGCTAAAAAGAAAAATCCCAAACCGGGAGAATTTGGCAGGGCAATGGCTCAAGAAATAATGATTGTGACAC
CTGCGATCGCAAACTTAATTAGAGAAGCAAAAGCCCCACAAATTTATTCTGCGATTCAAACAGGGGGTAAATTAGGAATG
CAAACGATGGAACAGGCTTTAGCAAATTTAGTAAAAACAGGTACAATTGCAGTGGAAGAAGCCCTTGCGAAAAGCAGTAA
ACCAGATGAATTAAAACGCTTATTAGGTTCTTCTATGGATAGCGGGATGGTTACTCCTGGTAAACGTCGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain ERS1305867

53.623

93.243

0.5
  pilT Legionella pneumophila strain Lp02

53.623

93.243

0.5
  pilT Pseudomonas aeruginosa PAK

52.45

93.784

0.492
  pilT Acinetobacter nosocomialis M2

51.585

93.784

0.484
  pilT Acinetobacter baumannii D1279779

51.585

93.784

0.484
  pilT Acinetobacter baumannii strain A118

51.585

93.784

0.484
  pilT Acinetobacter baylyi ADP1

51.297

93.784

0.481
  pilT Pseudomonas stutzeri DSM 10701

50.144

93.784

0.47
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.44

92.162

0.465
  pilT Vibrio cholerae strain A1552

50.44

92.162

0.465
  pilT Neisseria gonorrhoeae MS11

48.138

94.324

0.454
  pilT Neisseria meningitidis 8013

48.415

93.784

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.727

95.135

0.454
  pilU Pseudomonas stutzeri DSM 10701

37.741

98.108

0.37
  pilU Acinetobacter baylyi ADP1

35.34

100

0.365