Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LCS67_RS03690 Genome accession   NZ_CP084001
Coordinates   769344..770324 (+) Length   326 a.a.
NCBI ID   WP_001055636.1    Uniprot ID   A0A5I1ZS40
Organism   Salmonella sp. A7     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 764344..775324
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LCS67_RS03660 (LCS67_03660) - 764921..765928 (+) 1008 WP_000252191.1 DUF1202 domain-containing protein -
  LCS67_RS03665 (LCS67_03665) hemW 766020..767156 (-) 1137 WP_031566572.1 radical SAM family heme chaperone HemW -
  LCS67_RS03670 (LCS67_03670) - 767149..767742 (-) 594 WP_023195148.1 XTP/dITP diphosphatase -
  LCS67_RS03675 (LCS67_03675) yggU 767750..768040 (-) 291 WP_001277203.1 DUF167 family protein YggU -
  LCS67_RS03680 (LCS67_03680) - 768037..768603 (-) 567 WP_001094848.1 YggT family protein -
  LCS67_RS03685 (LCS67_03685) - 768622..769326 (-) 705 WP_000997790.1 YggS family pyridoxal phosphate-dependent enzyme -
  LCS67_RS03690 (LCS67_03690) pilT 769344..770324 (+) 981 WP_001055636.1 type IV pilus twitching motility protein PilT Machinery gene
  LCS67_RS03695 (LCS67_03695) - 770452..771153 (+) 702 WP_000098326.1 IclR family transcriptional regulator -
  LCS67_RS03700 (LCS67_03700) ruvX 771185..771601 (-) 417 WP_000017096.1 Holliday junction resolvase RuvX -
  LCS67_RS03705 (LCS67_03705) - 771601..772164 (-) 564 WP_001053175.1 YqgE/AlgH family protein -
  LCS67_RS03710 (LCS67_03710) gshB 772380..773327 (-) 948 WP_023137233.1 glutathione synthase -
  LCS67_RS03715 (LCS67_03715) rsmE 773347..774078 (-) 732 WP_001222488.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  LCS67_RS03720 (LCS67_03720) endA 774155..774862 (-) 708 WP_000286123.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36126.41 Da        Isoelectric Point: 7.5354

>NTDB_id=608875 LCS67_RS03690 WP_001055636.1 769344..770324(+) (pilT) [Salmonella sp. A7]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMEIAPFTAPDVDKLLLDWLNDAQQYQWRTHGQLDFAVSLSGTRRLRA
SAFTHQQGTSLALRLLPERCPDLAEIQMPPIVPALLASENGLILVTGATGCGKSTTLAAMVGHLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=608875 LCS67_RS03690 WP_001055636.1 769344..770324(+) (pilT) [Salmonella sp. A7]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGATCGCGCCTTTTACCGCGCCAGATGTCGACAAGCTTCTGCTTGATTGGCTTAATG
ACGCGCAACAATATCAGTGGCGAACGCACGGCCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTACGCGCC
AGTGCGTTCACACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGCGTTGTCCTGATCTGGCGGAAATCCA
GATGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCTACTGGCTGCGGGAAGT
CCACCACGCTGGCGGCGATGGTGGGACATCTTAATCAACATGCCGATAAACACATCCTGACGCTGGAAGATCCTATTGAG
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCGGCGTTGCGTGAAGATCCCGATGTCATTTTGCTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGTGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGGTACGCAGTCAACTGGCGGGTAGTCTGCGCGCTGTACTATCGCAAAAACTGGAGGT
CGACAGACAGGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGCGCAGGGACGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(76-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5I1ZS40

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.318

100

0.485
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.318

100

0.485
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Pseudomonas aeruginosa PAK

45.26

100

0.454
  pilT Pseudomonas stutzeri DSM 10701

44.954

100

0.451
  pilT Acinetobacter baylyi ADP1

44.954

100

0.451
  pilT Acinetobacter baumannii strain A118

44.648

100

0.448
  pilT Acinetobacter baumannii D1279779

44.648

100

0.448
  pilT Acinetobacter nosocomialis M2

44.343

100

0.445
  pilT Legionella pneumophila strain ERS1305867

44.969

97.546

0.439
  pilT Legionella pneumophila strain Lp02

44.969

97.546

0.439
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.453

97.546

0.414
  pilU Vibrio cholerae strain A1552

38.558

97.853

0.377
  pilU Pseudomonas stutzeri DSM 10701

35.65

100

0.362