Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LCD40_RS19715 Genome accession   NZ_CP083849
Coordinates   4070090..4071070 (+) Length   326 a.a.
NCBI ID   WP_017692691.1    Uniprot ID   A0A2J0PYR2
Organism   Enterobacter hormaechei subsp. steigerwaltii strain 14269-yvys     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4065090..4076070
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LCD40_RS19675 (LCD40_19695) - 4065173..4065499 (+) 327 WP_023304407.1 YggL family protein -
  LCD40_RS19680 (LCD40_19700) - 4065557..4066273 (+) 717 WP_023304406.1 DUF2884 domain-containing protein -
  LCD40_RS19685 (LCD40_19705) - 4066357..4066695 (-) 339 WP_023304405.1 endonuclease domain-containing protein -
  LCD40_RS19690 (LCD40_19710) hemW 4066752..4067900 (-) 1149 WP_017382926.1 radical SAM family heme chaperone HemW -
  LCD40_RS19695 (LCD40_19715) - 4067893..4068486 (-) 594 WP_015571839.1 XTP/dITP diphosphatase -
  LCD40_RS19700 (LCD40_19720) yggU 4068490..4068786 (-) 297 WP_023304404.1 DUF167 family protein YggU -
  LCD40_RS19705 (LCD40_19725) - 4068783..4069349 (-) 567 WP_015571838.1 YggT family protein -
  LCD40_RS19710 (LCD40_19730) - 4069371..4070072 (-) 702 WP_023304403.1 YggS family pyridoxal phosphate-dependent enzyme -
  LCD40_RS19715 (LCD40_19735) pilT 4070090..4071070 (+) 981 WP_017692691.1 type IV pilus twitching motility protein PilT Machinery gene
  LCD40_RS19720 (LCD40_19740) ruvX 4071080..4071496 (-) 417 WP_023304402.1 Holliday junction resolvase RuvX -
  LCD40_RS19725 (LCD40_19745) - 4071496..4072056 (-) 561 WP_006811924.1 YqgE/AlgH family protein -
  LCD40_RS19730 (LCD40_19750) gshB 4072131..4073078 (-) 948 WP_015571835.1 glutathione synthase -
  LCD40_RS19735 (LCD40_19755) rsmE 4073110..4073841 (-) 732 WP_017382923.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  LCD40_RS19740 (LCD40_19760) endA 4073893..4074600 (-) 708 WP_003860034.1 deoxyribonuclease I -
  LCD40_RS19745 (LCD40_19765) - 4074695..4075192 (-) 498 WP_015571833.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35682.70 Da        Isoelectric Point: 6.3047

>NTDB_id=608238 LCD40_RS19715 WP_017692691.1 4070090..4071070(+) (pilT) [Enterobacter hormaechei subsp. steigerwaltii strain 14269-yvys]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVEALLKAWLNDEQQGAWWANGQVDFAVTLAGRQRLRG
SAFKHMLGVSIALRLLPLTCPQLSALGVPRAIPELLSNDNGLILVTGATGSGKSTTLAAMVDFLNHQTDGHILTLEDPVE
FMYQSERCLIQQREIGQHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLRQDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=608238 LCD40_RS19715 WP_017692691.1 4070090..4071070(+) (pilT) [Enterobacter hormaechei subsp. steigerwaltii strain 14269-yvys]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGGTCAGGCCGTCTTGAACCTGCGCCGTTTCCGCCCCCGGATGTGGAAGCATTATTAAAAGCGTGGCTCAACG
ATGAACAGCAGGGCGCATGGTGGGCAAACGGGCAGGTGGATTTTGCCGTTACCCTCGCAGGCCGTCAGCGGCTGCGCGGC
AGTGCGTTTAAGCATATGCTCGGCGTTTCGATTGCGCTGCGGCTGTTGCCGCTGACGTGCCCGCAGCTCTCTGCGTTAGG
TGTGCCGCGAGCGATCCCGGAACTGCTGTCCAATGACAATGGCCTGATTCTGGTCACCGGCGCCACCGGCAGCGGGAAAT
CGACCACCCTGGCCGCTATGGTCGATTTCCTCAATCACCAGACGGACGGGCATATTCTGACCCTGGAAGATCCGGTGGAG
TTTATGTACCAGAGCGAACGTTGCCTGATCCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCCTTTGCCGAGGCGCTGCG
CAGCGCGCTGCGTGAAGATCCGGATGTGATTCTGCTTGGTGAGCTGCGCGACAGCGAGACGATACGCCTGGCGCTGACGG
CGGCGGAAACCGGCCATCTGGTGTTAGCCACGCTGCACACGCGCGGCGCGGCGCAGGCGATCGAGCGCCTGGTCGACACC
TTTCCGGCGCAGGAGAAAGATCCGGTGCGTAATCAGCTGGCGGGAAGCCTGCGTGCGGTCCTGGCGCAGAAGCTACGTCA
GGATGTCCAGGGCGGGCGCGTGGCGCTGTATGAGCTACTGGTGAATACCTCGGCGGCGGCAAACCTGATCCGCGAAGGCA
AAACCTGGCAACTGCCGGGCATTATTCAAACGGGGCAGCAGGCGGGAATGCAGAACTTTGAGCAGAGTCTGGCGGAGCGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(108-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2J0PYR2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Legionella pneumophila strain Lp02

48.758

98.773

0.482
  pilT Legionella pneumophila strain ERS1305867

48.758

98.773

0.482
  pilT Pseudomonas stutzeri DSM 10701

48.012

100

0.482
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Acinetobacter baumannii strain A118

47.095

100

0.472
  pilT Pseudomonas aeruginosa PAK

47.095

100

0.472
  pilT Acinetobacter baumannii D1279779

47.095

100

0.472
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

47.5

98.16

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.344

99.08

0.429
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.387
  pilU Vibrio cholerae strain A1552

37.888

98.773

0.374