Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LA317_RS02890 Genome accession   NZ_CP083640
Coordinates   580254..581279 (+) Length   341 a.a.
NCBI ID   WP_223398757.1    Uniprot ID   -
Organism   Enterobacter ludwigii strain FDAARGOS_1498     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 575254..586279
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LA317_RS02850 (LA317_02850) - 575335..575661 (+) 327 WP_032678951.1 YggL family protein -
  LA317_RS02855 (LA317_02855) - 575718..576434 (+) 717 WP_223398754.1 DUF2884 domain-containing protein -
  LA317_RS02860 (LA317_02860) - 576496..576858 (-) 363 WP_138614617.1 DUF559 domain-containing protein -
  LA317_RS02865 (LA317_02865) hemW 576922..578064 (-) 1143 WP_223398755.1 radical SAM family heme chaperone HemW -
  LA317_RS02870 (LA317_02870) - 578057..578650 (-) 594 WP_014171572.1 XTP/dITP diphosphatase -
  LA317_RS02875 (LA317_02875) yggU 578654..578950 (-) 297 WP_014885193.1 DUF167 family protein YggU -
  LA317_RS02880 (LA317_02880) - 578947..579513 (-) 567 WP_014171570.1 YggT family protein -
  LA317_RS02885 (LA317_02885) - 579535..580236 (-) 702 WP_223398756.1 YggS family pyridoxal phosphate-dependent enzyme -
  LA317_RS02890 (LA317_02890) pilT 580254..581279 (+) 1026 WP_223398757.1 type IV pilus twitching motility protein PilT Machinery gene
  LA317_RS02895 (LA317_02895) ruvX 581245..581661 (-) 417 WP_014171567.1 Holliday junction resolvase RuvX -
  LA317_RS02900 (LA317_02900) - 581661..582221 (-) 561 WP_020883695.1 YqgE/AlgH family protein -
  LA317_RS02905 (LA317_02905) gshB 582308..583255 (-) 948 WP_020883696.1 glutathione synthase -
  LA317_RS02910 (LA317_02910) rsmE 583295..584026 (-) 732 WP_020883697.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  LA317_RS02915 (LA317_02915) endA 584079..584786 (-) 708 WP_073396422.1 deoxyribonuclease I -
  LA317_RS02920 (LA317_02920) - 584881..585378 (-) 498 WP_032670169.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 37105.11 Da        Isoelectric Point: 5.8611

>NTDB_id=607017 LA317_RS02890 WP_223398757.1 580254..581279(+) (pilT) [Enterobacter ludwigii strain FDAARGOS_1498]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRTGRLEPAPFPPPEVDTLLKAWLNDEQQGTWWANGQVDFAVTLAGNQRLRG
SAFKQTSGVSMTLRLLPGVCPQLSALGVPQVVPELLSSDNGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
FLYQSERCLIQQREIGQHSPSFADALRGALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLLPDAQGGRVALYELLVNTPAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGQLQAPALNNPVRNSFRE

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=607017 LA317_RS02890 WP_223398757.1 580254..581279(+) (pilT) [Enterobacter ludwigii strain FDAARGOS_1498]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAATGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGAACAGGTCGTCTTGAACCCGCGCCGTTTCCGCCTCCCGAAGTCGATACGCTATTAAAGGCGTGGCTCAACG
ATGAACAGCAGGGAACGTGGTGGGCGAACGGGCAGGTGGATTTTGCCGTCACCCTGGCGGGCAATCAGCGCCTGCGCGGA
AGCGCGTTTAAACAGACAAGCGGCGTCTCAATGACGCTGCGGCTGTTGCCCGGGGTCTGCCCGCAACTCTCCGCGTTGGG
GGTGCCCCAGGTTGTTCCTGAGCTGTTATCCAGTGACAACGGGCTAATTCTGGTCACCGGGGCAACCGGCAGCGGTAAGT
CCACTACGCTGGCAGCGATGGTGGATTTTCTTAATCACCATACTGACGGGCATATTCTGACGCTCGAAGACCCGGTGGAG
TTTCTGTACCAGAGCGAACGGTGCCTGATCCAGCAGCGCGAGATTGGCCAACACAGCCCGTCATTTGCCGACGCGCTGCG
CGGGGCGTTACGCCAGGATCCTGATGTCATACTGCTGGGAGAGCTGCGCGACAGTGAAACAATCCGCCTCGCGCTGACGG
CGGCAGAAACCGGACATCTGGTGCTCGCGACACTGCATACCCGCGGGGCAGCGCAGGCGATTGAGCGGCTGGTCGATACG
TTTCCGGCGCAGGAGAAAGATCCGGTGCGCAATCAGCTGGCCGGCAGCCTGCGGGCGGTGCTGGCGCAAAAGCTCCTCCC
CGATGCGCAGGGCGGGCGCGTGGCGCTTTATGAGCTGCTGGTGAATACTCCGGCGGCGGCGAATCTCATTCGCGAAGGAA
AAACGTGGCAATTGCCCGGTATTATTCAAACCGGCCAGCAGGCGGGGATGCAGAACTTTGACCAGAGCCTGGCTGAAAGA
CGGGCGCAGGGGCAACTCCAGGCCCCGGCGCTCAATAACCCTGTTCGAAATAGCTTTCGAGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(110-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.112

99.12

0.487
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.112

99.12

0.487
  pilT Neisseria meningitidis 8013

48.348

97.654

0.472
  pilT Neisseria gonorrhoeae MS11

46.784

100

0.469
  pilT Legionella pneumophila strain ERS1305867

49.068

94.428

0.463
  pilT Legionella pneumophila strain Lp02

49.068

94.428

0.463
  pilT Acinetobacter baylyi ADP1

46.988

97.361

0.457
  pilT Pseudomonas stutzeri DSM 10701

47.706

95.894

0.457
  pilT Acinetobacter baumannii D1279779

47.706

95.894

0.457
  pilT Acinetobacter baumannii strain A118

47.706

95.894

0.457
  pilT Pseudomonas aeruginosa PAK

47.401

95.894

0.455
  pilT Acinetobacter nosocomialis M2

48.125

93.842

0.452
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.72

94.428

0.422
  pilU Pseudomonas stutzeri DSM 10701

40.303

96.774

0.39
  pilU Vibrio cholerae strain A1552

39.441

94.428

0.372