Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   K9O83_RS18600 Genome accession   NZ_CP083403
Coordinates   3924884..3925864 (-) Length   326 a.a.
NCBI ID   WP_224065989.1    Uniprot ID   -
Organism   Enterobacter bugandensis strain XL95     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3919884..3930864
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K9O83_RS18570 (K9O83_18510) - 3920774..3921271 (+) 498 WP_136485047.1 SprT family zinc-dependent metalloprotease -
  K9O83_RS18575 (K9O83_18515) endA 3921366..3922073 (+) 708 WP_028014365.1 deoxyribonuclease I -
  K9O83_RS18580 (K9O83_18520) rsmE 3922125..3922856 (+) 732 WP_100170841.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  K9O83_RS18585 (K9O83_18525) gshB 3922876..3923823 (+) 948 WP_224065988.1 glutathione synthase -
  K9O83_RS18590 (K9O83_18530) - 3923898..3924458 (+) 561 WP_028014368.1 YqgE/AlgH family protein -
  K9O83_RS18595 (K9O83_18535) ruvX 3924458..3924874 (+) 417 WP_021242076.1 Holliday junction resolvase RuvX -
  K9O83_RS18600 (K9O83_18540) pilT 3924884..3925864 (-) 981 WP_224065989.1 type IV pilus twitching motility protein PilT Machinery gene
  K9O83_RS18605 (K9O83_18545) - 3925882..3926583 (+) 702 WP_224065990.1 YggS family pyridoxal phosphate-dependent enzyme -
  K9O83_RS18610 (K9O83_18550) - 3926605..3927171 (+) 567 WP_045260768.1 YggT family protein -
  K9O83_RS18615 (K9O83_18555) yggU 3927168..3927464 (+) 297 WP_047364545.1 DUF167 family protein YggU -
  K9O83_RS18620 (K9O83_18560) - 3927468..3928061 (+) 594 WP_010435473.1 XTP/dITP diphosphatase -
  K9O83_RS18625 (K9O83_18565) hemW 3928054..3929196 (+) 1143 WP_224065991.1 radical SAM family heme chaperone HemW -
  K9O83_RS18630 (K9O83_18570) - 3929401..3930117 (-) 717 WP_041908321.1 DUF2884 domain-containing protein -
  K9O83_RS18635 (K9O83_18575) - 3930175..3930501 (-) 327 WP_028014375.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35540.63 Da        Isoelectric Point: 6.1627

>NTDB_id=605656 K9O83_RS18600 WP_224065989.1 3924884..3925864(-) (pilT) [Enterobacter bugandensis strain XL95]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRLGRLEPAPFPPPDVGALLKAWLNDEQQGAWWANGQVDFAVTVAGDQRLRG
SAFKQMKGISVTLRLLPRSCPQLPSLGVPRAIPELLSSDNGLILVTGATGSGKSTTLAAMVDFLNRHTDGHILTLEDPVE
FMYQSERCLIQQREIGQHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLLPDLQGGLVALYELLVNTAAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=605656 K9O83_RS18600 WP_224065989.1 3924884..3925864(-) (pilT) [Enterobacter bugandensis strain XL95]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCACCACCGCG
CTGGCGCAGATTGGGCCGCCTTGAGCCGGCCCCGTTTCCGCCGCCGGATGTCGGGGCGTTGCTAAAGGCGTGGCTCAACG
ACGAACAGCAGGGCGCATGGTGGGCAAACGGGCAGGTCGATTTTGCCGTGACGGTCGCAGGAGACCAGCGTCTGCGCGGC
AGTGCGTTTAAGCAGATGAAGGGCATCTCCGTTACCCTGCGGCTGTTGCCGCGTTCCTGTCCGCAGCTCCCTTCACTTGG
GGTACCGCGCGCTATTCCCGAACTGTTGTCCAGCGACAACGGGTTGATTCTGGTAACGGGAGCGACCGGCAGCGGTAAAT
CCACCACGCTGGCCGCAATGGTCGACTTCCTCAACCGCCATACTGACGGCCATATCCTCACGCTTGAAGATCCGGTGGAG
TTTATGTACCAGAGTGAACGTTGCCTGATTCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCATTTGCAGAGGCGCTGCG
CAGTGCGTTACGCGAGGACCCGGACGTTATTTTGCTGGGGGAGCTGCGCGACAGCGAAACGATCCGCCTGGCGCTGACGG
CGGCGGAGACCGGGCATCTGGTGCTGGCGACGCTGCACACGCGCGGGGCAGCACAGGCGATTGAACGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCTGGCAGCCTGCGGGCAGTGCTGGCGCAAAAGCTGCTTCC
CGATCTTCAGGGCGGGCTCGTCGCGCTGTACGAGCTGCTGGTAAACACCGCGGCGGCGGCGAATCTGATTCGTGAGGGGA
AGACGTGGCAGCTGCCCGGCATCATTCAAACCGGCCAGCAGGCGGGAATGCAGAACTTTGACCAGAGCCTGGCGGAGAGA
CGGGCACAGGGGCGGCTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(90-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.153

100

0.503
  pilT Vibrio cholerae strain A1552

50.153

100

0.503
  pilT Pseudomonas stutzeri DSM 10701

48.624

100

0.488
  pilT Legionella pneumophila strain Lp02

49.379

98.773

0.488
  pilT Legionella pneumophila strain ERS1305867

49.379

98.773

0.488
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Acinetobacter baumannii strain A118

48.012

100

0.482
  pilT Acinetobacter baumannii D1279779

48.012

100

0.482
  pilT Acinetobacter baylyi ADP1

48.012

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Pseudomonas aeruginosa PAK

47.706

100

0.479
  pilT Acinetobacter nosocomialis M2

48.438

98.16

0.475
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.547

98.773

0.42
  pilU Pseudomonas stutzeri DSM 10701

38.788

100

0.393
  pilU Vibrio cholerae strain A1552

39.13

98.773

0.386