Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   K7G97_RS11835 Genome accession   NZ_CP082333
Coordinates   2256376..2257422 (-) Length   348 a.a.
NCBI ID   WP_214771042.1    Uniprot ID   -
Organism   Exiguobacterium acetylicum strain EYLC1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2251376..2262422
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K7G97_RS11815 (K7G97_11815) - 2251943..2253670 (-) 1728 WP_223040645.1 fimbrial assembly protein -
  K7G97_RS11820 (K7G97_11820) - 2253689..2254444 (-) 756 WP_223040646.1 A24 family peptidase -
  K7G97_RS11825 (K7G97_11825) - 2254501..2255016 (-) 516 WP_223040647.1 type II secretion system protein -
  K7G97_RS11830 (K7G97_11830) - 2255154..2256371 (-) 1218 WP_223040648.1 type II secretion system F family protein -
  K7G97_RS11835 (K7G97_11835) pilT 2256376..2257422 (-) 1047 WP_214771042.1 type IV pilus twitching motility protein PilT Machinery gene
  K7G97_RS11840 (K7G97_11840) - 2257419..2259083 (-) 1665 WP_223040649.1 GspE/PulE family protein -
  K7G97_RS11845 (K7G97_11845) - 2259092..2259406 (-) 315 WP_029342268.1 hypothetical protein -
  K7G97_RS11850 (K7G97_11850) - 2259422..2260783 (-) 1362 WP_262415746.1 G5 domain-containing protein -
  K7G97_RS11855 (K7G97_11855) - 2260974..2261501 (+) 528 WP_223040650.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  K7G97_RS11860 (K7G97_11860) - 2261464..2262084 (+) 621 WP_029342271.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 38155.90 Da        Isoelectric Point: 7.1317

>NTDB_id=601557 K7G97_RS11835 WP_214771042.1 2256376..2257422(-) (pilT) [Exiguobacterium acetylicum strain EYLC1]
MNSTMERAQIEDILRSSKERGASDVHLTAGSPPVFRINGTLTPFGTEKMKPIIIDGFVQSLITEEMYLQLKQDRDIDFSF
GVTGVARYRVNCFYQRGALSMAFRTIPTEVPTLEQLALPPVLKRFLTKPHGLILVTGPTGSGKSTTLAAMINEINQTMYK
RIITLEDPIEYLHSHHKSLIDQREIGIDAPRFATGLRSALRQDPDVILLGEMRDLETIATAVTAAETGHLVFATVHTSTA
ASTVSRIIDVFPSEQQDQIRAQLANVLAGVVSQRLMPRSDGTGRVAALEIMVDTPAVSNLIRTGKEYQIQSVLQTGKQYG
MQTMQMALQDLVSRGLIPASAASPYISG

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=601557 K7G97_RS11835 WP_214771042.1 2256376..2257422(-) (pilT) [Exiguobacterium acetylicum strain EYLC1]
ATGAACAGTACGATGGAACGGGCACAAATCGAAGATATTTTACGCTCATCAAAAGAACGAGGAGCGTCCGATGTTCACTT
GACGGCGGGATCACCACCCGTCTTTCGAATCAACGGAACGTTGACACCGTTTGGGACTGAAAAGATGAAACCAATCATCA
TTGATGGTTTTGTGCAGTCACTGATCACGGAAGAGATGTACCTACAGCTGAAGCAGGACCGGGATATCGACTTTTCGTTC
GGTGTGACCGGTGTCGCTCGGTACCGGGTCAACTGTTTTTACCAACGGGGCGCTTTGTCGATGGCGTTTCGGACGATTCC
GACCGAGGTGCCGACGCTTGAGCAATTAGCGTTACCGCCTGTCTTAAAACGATTCTTAACGAAACCACACGGCTTGATTC
TCGTGACGGGTCCGACGGGTTCCGGGAAATCGACGACGCTTGCAGCGATGATCAATGAAATTAATCAGACGATGTATAAA
CGGATCATTACGCTGGAGGATCCGATTGAGTATTTACATAGTCATCATAAAAGCCTGATTGATCAGCGGGAAATCGGGAT
TGATGCGCCACGTTTCGCGACCGGGTTACGGTCAGCACTCCGTCAAGATCCGGATGTCATTTTGCTTGGAGAGATGCGAG
ACCTTGAAACGATCGCGACAGCTGTGACAGCAGCAGAGACAGGTCACCTTGTCTTCGCGACCGTCCATACGTCAACGGCT
GCCTCGACTGTCAGCCGGATCATCGATGTCTTTCCTTCAGAGCAACAAGACCAGATTCGGGCGCAACTGGCGAACGTTCT
CGCGGGTGTCGTCTCGCAACGCCTGATGCCGCGTTCGGATGGAACAGGACGCGTCGCAGCCCTTGAGATCATGGTCGATA
CACCAGCTGTCTCGAACTTGATTCGGACCGGTAAGGAATATCAGATTCAATCCGTCCTGCAGACGGGTAAACAATACGGC
ATGCAGACGATGCAGATGGCATTGCAGGATCTCGTCAGTCGGGGATTGATTCCGGCTTCTGCTGCATCACCGTACATATC
GGGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.524

94.253

0.486
  pilT Neisseria meningitidis 8013

50

95.977

0.48
  pilT Neisseria gonorrhoeae MS11

50

95.977

0.48
  pilT Acinetobacter nosocomialis M2

48.949

95.69

0.468
  pilT Pseudomonas aeruginosa PAK

48.949

95.69

0.468
  pilT Acinetobacter baylyi ADP1

47.941

97.701

0.468
  pilT Acinetobacter baumannii D1279779

48.649

95.69

0.466
  pilT Acinetobacter baumannii strain A118

48.649

95.69

0.466
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.091

94.828

0.466
  pilT Vibrio cholerae strain A1552

49.091

94.828

0.466
  pilT Pseudomonas stutzeri DSM 10701

47.748

95.69

0.457
  pilT Legionella pneumophila strain Lp02

46.246

95.69

0.443
  pilT Legionella pneumophila strain ERS1305867

46.246

95.69

0.443
  pilU Pseudomonas stutzeri DSM 10701

40.426

94.54

0.382
  pilU Acinetobacter baylyi ADP1

40.06

95.402

0.382