Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   K6748_RS02360 Genome accession   NZ_CP082307
Coordinates   491610..492650 (+) Length   346 a.a.
NCBI ID   WP_205461127.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain HNV2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 486610..497650
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6748_RS02330 (K6748_02330) - 487721..488323 (-) 603 WP_005381009.1 XTP/dITP diphosphatase -
  K6748_RS02335 (K6748_02335) - 488442..488873 (-) 432 WP_005381010.1 DUF4426 domain-containing protein -
  K6748_RS02340 (K6748_02340) yggU 489002..489292 (-) 291 WP_005381011.1 DUF167 family protein YggU -
  K6748_RS02345 (K6748_02345) - 489292..489849 (-) 558 WP_005381012.1 YggT family protein -
  K6748_RS02350 (K6748_02350) proC 489902..490720 (-) 819 WP_042521294.1 pyrroline-5-carboxylate reductase -
  K6748_RS02355 (K6748_02355) - 490880..491581 (-) 702 WP_042521292.1 YggS family pyridoxal phosphate-dependent enzyme -
  K6748_RS02360 (K6748_02360) pilT 491610..492650 (+) 1041 WP_205461127.1 type IV pilus twitching motility protein PilT Machinery gene
  K6748_RS02365 (K6748_02365) pilU 492681..493787 (+) 1107 WP_005381016.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K6748_RS02370 (K6748_02370) ruvX 493848..494273 (-) 426 WP_005381017.1 Holliday junction resolvase RuvX -
  K6748_RS02375 (K6748_02375) - 494309..494872 (-) 564 WP_005381020.1 YqgE/AlgH family protein -
  K6748_RS02380 (K6748_02380) gshB 495000..495950 (-) 951 WP_005381021.1 glutathione synthase -
  K6748_RS02385 (K6748_02385) rsmE 495964..496695 (-) 732 WP_205461128.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  K6748_RS02390 (K6748_02390) endA 496809..497504 (-) 696 WP_017635582.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38502.00 Da        Isoelectric Point: 6.5039

>NTDB_id=601178 K6748_RS02360 WP_205461127.1 491610..492650(+) (pilT) [Vibrio alginolyticus strain HNV2]
MDITELLDFSVKHNASDLHLSAGVPPMVRIDGDVRKLGIPAFTHQEVHRLVFEIMNDAQRSEFEEKLEVDFSFELHNVGR
FRVNAFNQSRGCAAVFRTIPSSIPTLEELEAPEIFKKIANAEKGLVLVTGPTGSGKSTTLAAIVDYINRNHNKHVLTIED
PIEFVHNNNKCLINQREVHRDTHSFQNALRSALREDPDVILVGEMRDKETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRNGGGRIACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQAMEQNA
RQLMAQGMVAREEVDSKIGLEVQQFS

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=601178 K6748_RS02360 WP_205461127.1 491610..492650(+) (pilT) [Vibrio alginolyticus strain HNV2]
ATGGATATCACTGAGTTACTGGATTTTAGTGTAAAACATAATGCCTCGGATCTACATCTTTCTGCAGGTGTCCCACCAAT
GGTTCGTATTGATGGGGATGTAAGAAAACTAGGCATTCCAGCGTTTACACACCAAGAAGTGCATCGTTTGGTTTTCGAAA
TCATGAACGATGCCCAGCGCAGTGAGTTCGAAGAAAAACTCGAAGTCGATTTCTCTTTTGAGTTGCATAACGTTGGCCGT
TTCCGTGTCAACGCTTTCAACCAATCTCGCGGTTGTGCGGCAGTATTCCGTACCATTCCAAGTAGCATTCCAACTCTGGA
AGAGCTAGAAGCGCCAGAGATCTTCAAAAAAATTGCGAACGCTGAAAAAGGCTTAGTTCTGGTCACTGGCCCAACAGGTT
CAGGTAAATCGACGACCTTAGCAGCCATTGTTGACTACATTAACCGTAATCACAACAAGCACGTACTCACAATTGAAGAT
CCGATTGAATTTGTTCACAACAACAATAAATGTTTGATTAACCAGCGTGAAGTTCACCGTGACACCCATAGTTTCCAAAA
CGCACTGCGCAGCGCATTGCGCGAAGACCCAGACGTTATTCTGGTTGGTGAGATGCGTGACAAAGAAACCATCAGCCTTG
CCTTAACGGCGGCAGAAACGGGTCACTTAGTTTTCGGGACGTTGCACACCAGCTCGGCGGCAAAAACCATTGACCGTATT
ATCGATGTGTTTCCTGGTAGTGATAAAGATATGGTTCGCTCGATGCTATCTGAGTCCCTTCGTTCGGTTATCGCGCAAAA
GCTTCTGAAGCGTAATGGTGGTGGCCGTATTGCATGCCATGAAATTATGATGGCAACGCCTGCGATTCGCAACTTGATCC
GCGAAGACAAAGTCGCGCAGATGTATTCGATCATTCAAACAGGTGCGGCTCACGGCATGCAAGCCATGGAGCAAAATGCT
CGTCAGTTAATGGCGCAAGGCATGGTTGCTCGTGAAGAGGTCGATAGTAAGATCGGATTAGAAGTGCAGCAATTCTCGTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

86.667

99.711

0.864
  pilT Vibrio cholerae strain A1552

86.667

99.711

0.864
  pilT Acinetobacter baumannii D1279779

71.818

95.376

0.685
  pilT Acinetobacter baumannii strain A118

71.818

95.376

0.685
  pilT Acinetobacter nosocomialis M2

71.515

95.376

0.682
  pilT Acinetobacter baylyi ADP1

71.125

95.087

0.676
  pilT Pseudomonas aeruginosa PAK

68.843

97.399

0.671
  pilT Pseudomonas stutzeri DSM 10701

68.546

97.399

0.668
  pilT Legionella pneumophila strain Lp02

67.372

95.665

0.645
  pilT Legionella pneumophila strain ERS1305867

67.372

95.665

0.645
  pilT Neisseria meningitidis 8013

65.465

96.243

0.63
  pilT Neisseria gonorrhoeae MS11

65.165

96.243

0.627
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.516

91.908

0.483
  pilU Vibrio cholerae strain A1552

41.018

96.532

0.396
  pilU Pseudomonas stutzeri DSM 10701

40.419

96.532

0.39