Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HU826_RS17685 Genome accession   NZ_CP082280
Coordinates   3767732..3768712 (-) Length   326 a.a.
NCBI ID   WP_153682044.1    Uniprot ID   -
Organism   Enterobacter cancerogenus strain HAEC1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3762732..3773712
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HU826_RS17655 (HU826_17655) - 3763589..3764086 (+) 498 WP_058608620.1 SprT family zinc-dependent metalloprotease -
  HU826_RS17660 (HU826_17660) endA 3764181..3764888 (+) 708 WP_006178442.1 deoxyribonuclease I -
  HU826_RS17665 (HU826_17665) rsmE 3764941..3765672 (+) 732 WP_042321930.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  HU826_RS17670 (HU826_17670) gshB 3765694..3766641 (+) 948 WP_042322311.1 glutathione synthase -
  HU826_RS17675 (HU826_17675) - 3766724..3767284 (+) 561 WP_006178445.1 YqgE/AlgH family protein -
  HU826_RS17680 (HU826_17680) ruvX 3767284..3767700 (+) 417 WP_006178446.1 Holliday junction resolvase RuvX -
  HU826_RS17685 (HU826_17685) pilT 3767732..3768712 (-) 981 WP_153682044.1 type IV pilus twitching motility protein PilT Machinery gene
  HU826_RS17690 (HU826_17690) - 3768730..3769443 (+) 714 WP_058608622.1 YggS family pyridoxal phosphate-dependent enzyme -
  HU826_RS17695 (HU826_17695) - 3769452..3770018 (+) 567 WP_058608623.1 YggT family protein -
  HU826_RS17700 (HU826_17700) yggU 3770015..3770311 (+) 297 WP_006178450.1 DUF167 family protein YggU -
  HU826_RS17705 (HU826_17705) - 3770315..3770908 (+) 594 WP_058608624.1 XTP/dITP diphosphatase -
  HU826_RS17710 (HU826_17710) hemW 3770901..3772043 (+) 1143 WP_058608625.1 radical SAM family heme chaperone HemW -
  HU826_RS17715 (HU826_17715) - 3772107..3772445 (+) 339 WP_058608647.1 endonuclease domain-containing protein -
  HU826_RS17720 (HU826_17720) - 3772550..3773266 (-) 717 WP_058608626.1 DUF2884 domain-containing protein -
  HU826_RS17725 (HU826_17725) - 3773324..3773650 (-) 327 WP_006178455.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35814.89 Da        Isoelectric Point: 6.0414

>NTDB_id=600988 HU826_RS17685 WP_153682044.1 3767732..3768712(-) (pilT) [Enterobacter cancerogenus strain HAEC1]
MDVEEIVALSVKHNVSDLHLCSDAPPRWRRLGRLEPAPFPPPDVNVLLREWLNDEQQGVWWANGQVDFAVTTGENQRLRG
SAFMHMQGASVVLRLLPRACPQLSALGVPRAIPELLLNDSGLLLVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
FMYQSERCLIQQREIGQHSPTFAEALRGALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRTQLAGSLRAVLAQKLRPDLQGGRVALYELLVNTPAAANLIREGKTWQLPGIVQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=600988 HU826_RS17685 WP_153682044.1 3767732..3768712(-) (pilT) [Enterobacter cancerogenus strain HAEC1]
ATGGATGTGGAAGAAATCGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATGCCCCCCCGCG
CTGGCGGCGATTAGGCCGCCTTGAGCCCGCCCCGTTTCCGCCTCCGGACGTGAACGTGCTGCTCAGGGAGTGGCTTAATG
ATGAACAGCAGGGGGTTTGGTGGGCAAACGGGCAGGTGGATTTTGCCGTCACCACCGGCGAAAACCAGCGCCTGCGCGGT
AGTGCGTTTATGCATATGCAGGGTGCCTCTGTCGTGCTGCGACTGCTGCCGCGTGCCTGCCCGCAACTTTCTGCGTTGGG
CGTGCCGCGGGCGATCCCGGAGCTGTTACTGAATGACAGCGGGCTGCTGCTGGTGACCGGGGCAACCGGCAGCGGAAAAT
CCACCACTCTGGCGGCGATGGTCGATTTTCTCAACCACCACACCGACGGGCATATTCTGACGCTCGAAGATCCGGTGGAA
TTTATGTATCAGAGTGAACGCTGCCTGATCCAGCAGCGCGAGATAGGCCAGCACAGCCCGACGTTTGCCGAGGCGCTGCG
GGGCGCGTTGCGTGAAGATCCGGATGTGATTTTGCTGGGGGAGCTGCGCGACAGCGAAACGATCCGCCTGGCGCTAACGG
CGGCAGAAACCGGGCACCTGGTGCTGGCAACGCTGCATACGCGTGGGGCATCTCAGGCCATTGAACGGCTGGTGGATACC
TTTCCGGCGCAGGAGAAAGACCCGGTGCGCACCCAGCTGGCGGGCAGCCTGCGGGCGGTTCTGGCGCAAAAGCTGCGCCC
GGATTTGCAGGGCGGGCGCGTCGCGCTTTATGAGCTGCTGGTGAATACCCCGGCGGCGGCGAACCTGATCCGTGAAGGCA
AAACCTGGCAGCTTCCCGGGATCGTTCAAACGGGTCAGCAGGCGGGCATGCAGAACTTTGAACAGAGCCTGGCTGAGCGC
CGGGCGCAGGGCAGGCTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(60-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.847

100

0.5
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.847

100

0.5
  pilT Acinetobacter baylyi ADP1

47.401

100

0.475
  pilT Pseudomonas stutzeri DSM 10701

47.095

100

0.472
  pilT Acinetobacter baumannii D1279779

47.095

100

0.472
  pilT Acinetobacter baumannii strain A118

47.095

100

0.472
  pilT Legionella pneumophila strain ERS1305867

47.826

98.773

0.472
  pilT Legionella pneumophila strain Lp02

47.826

98.773

0.472
  pilT Neisseria meningitidis 8013

46.646

100

0.469
  pilT Acinetobacter nosocomialis M2

47.5

98.16

0.466
  pilT Pseudomonas aeruginosa PAK

46.483

100

0.466
  pilT Neisseria gonorrhoeae MS11

46.341

100

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.857

98.773

0.423
  pilU Pseudomonas stutzeri DSM 10701

39.091

100

0.396
  pilU Vibrio cholerae strain A1552

38.199

98.773

0.377