Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   K6982_RS14235 Genome accession   NZ_CP082215
Coordinates   3329076..3329483 (-) Length   135 a.a.
NCBI ID   WP_159407404.1    Uniprot ID   -
Organism   Xanthomonas cucurbitae strain MI_359     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3324076..3334483
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6982_RS14225 (K6982_14155) pilR 3325002..3326393 (+) 1392 WP_167526932.1 sigma-54 dependent transcriptional regulator Regulator
  K6982_RS14230 (K6982_14160) pilB 3327214..3328950 (-) 1737 WP_159407403.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K6982_RS14235 (K6982_14165) pilA/pilAI 3329076..3329483 (-) 408 WP_159407404.1 pilin Machinery gene
  K6982_RS14240 (K6982_14170) pilC 3329837..3331090 (+) 1254 WP_159407405.1 type II secretion system F family protein Machinery gene
  K6982_RS14245 (K6982_14175) - 3331097..3331960 (+) 864 WP_159407406.1 A24 family peptidase -
  K6982_RS14250 (K6982_14180) coaE 3331974..3332579 (+) 606 WP_104604291.1 dephospho-CoA kinase -
  K6982_RS14255 (K6982_14185) - 3332794..3334128 (-) 1335 WP_159407407.1 HAMP domain-containing sensor histidine kinase -

Sequence

Protein


Download         Length: 135 a.a.        Molecular weight: 14017.05 Da        Isoelectric Point: 8.4717

>NTDB_id=600253 K6982_RS14235 WP_159407404.1 3329076..3329483(-) (pilA/pilAI) [Xanthomonas cucurbitae strain MI_359]
MKKQQGFTLIELMIVVAIIAILAAIALPAYQDYVVKSQAASALAEITPGKVGFEQATNEGKTPSTTATDAGYIGVGGTTS
YCTVTVTANTIVCATRGGNATKFNGKNLTWTRDAATGLWSCGSDLDAKYKPGKCT

Nucleotide


Download         Length: 408 bp        

>NTDB_id=600253 K6982_RS14235 WP_159407404.1 3329076..3329483(-) (pilA/pilAI) [Xanthomonas cucurbitae strain MI_359]
ATGAAGAAGCAGCAGGGTTTTACGCTGATCGAACTGATGATCGTTGTTGCGATCATTGCTATTCTGGCCGCCATCGCGCT
GCCGGCCTATCAGGACTATGTGGTCAAATCGCAGGCCGCTTCGGCGCTCGCTGAAATCACCCCGGGAAAGGTTGGTTTCG
AGCAGGCAACCAATGAAGGCAAGACTCCAAGCACCACCGCGACTGATGCGGGCTACATTGGTGTCGGCGGAACCACCTCT
TACTGCACGGTCACTGTGACTGCGAACACCATCGTTTGCGCGACTCGTGGCGGAAACGCAACGAAGTTTAACGGAAAGAA
CCTCACCTGGACGCGCGACGCCGCCACCGGTCTCTGGAGCTGTGGATCCGATCTGGATGCGAAGTACAAGCCTGGCAAGT
GCACCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

69.63

100

0.696
  pilA/pilAII Pseudomonas stutzeri DSM 10701

61.654

98.519

0.607
  pilA Pseudomonas aeruginosa PAK

44

100

0.489
  pilA Acinetobacter baumannii strain A118

44.056

100

0.467
  pilE Neisseria gonorrhoeae MS11

36.709

100

0.43
  pilA Vibrio parahaemolyticus RIMD 2210633

43.939

97.778

0.43
  comP Acinetobacter baylyi ADP1

39.31

100

0.422
  pilA Vibrio cholerae C6706

39.437

100

0.415
  pilA Vibrio cholerae strain A1552

39.437

100

0.415
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

39.437

100

0.415
  pilA Ralstonia pseudosolanacearum GMI1000

34.177

100

0.4
  pilA2 Legionella pneumophila str. Paris

38.686

100

0.393
  pilA2 Legionella pneumophila strain ERS1305867

38.686

100

0.393