Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   K6982_RS14230 Genome accession   NZ_CP082215
Coordinates   3327214..3328950 (-) Length   578 a.a.
NCBI ID   WP_159407403.1    Uniprot ID   -
Organism   Xanthomonas cucurbitae strain MI_359     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3322214..3333950
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6982_RS14220 (K6982_14150) - 3323229..3324842 (+) 1614 WP_104604280.1 HAMP domain-containing sensor histidine kinase -
  K6982_RS14225 (K6982_14155) pilR 3325002..3326393 (+) 1392 WP_167526932.1 sigma-54 dependent transcriptional regulator Regulator
  K6982_RS14230 (K6982_14160) pilB 3327214..3328950 (-) 1737 WP_159407403.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K6982_RS14235 (K6982_14165) pilA/pilAI 3329076..3329483 (-) 408 WP_159407404.1 pilin Machinery gene
  K6982_RS14240 (K6982_14170) pilC 3329837..3331090 (+) 1254 WP_159407405.1 type II secretion system F family protein Machinery gene
  K6982_RS14245 (K6982_14175) - 3331097..3331960 (+) 864 WP_159407406.1 A24 family peptidase -
  K6982_RS14250 (K6982_14180) coaE 3331974..3332579 (+) 606 WP_104604291.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62727.97 Da        Isoelectric Point: 5.9537

>NTDB_id=600252 K6982_RS14230 WP_159407403.1 3327214..3328950(-) (pilB) [Xanthomonas cucurbitae strain MI_359]
MSVAGAANLVGITGIARRLVQDGALEELAARTAMEQAAAAKVPLPQWFVEKKLVSAAQLAAANAVEFGMPLMDVSVFDAS
QNAIKLVSEELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNASLGSSLG
GDDEEMGDLDVSAGDEDMGAGGDAGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLSQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIEKLGYEADQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKTSLP
EHALLAEGFTQQQIDQGITLYEAVGCDDCTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALM
KAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=600252 K6982_RS14230 WP_159407403.1 3327214..3328950(-) (pilB) [Xanthomonas cucurbitae strain MI_359]
ATGAGTGTTGCTGGAGCAGCCAATTTGGTTGGCATCACAGGGATTGCTCGCCGACTAGTTCAGGATGGCGCGCTGGAGGA
GTTGGCTGCACGTACCGCCATGGAACAAGCCGCTGCGGCTAAGGTACCTTTGCCGCAGTGGTTCGTCGAAAAGAAGCTTG
TGTCAGCGGCACAGTTGGCGGCGGCAAACGCAGTCGAGTTCGGCATGCCACTGATGGACGTATCGGTGTTCGACGCCAGC
CAGAACGCGATCAAGCTGGTCAGCGAGGAGTTGCTCCAGAAGCACCAGGTGCTGCCGCTGTTCAAGCGCGGGAACCGGTT
GTTCGTGGGGGTGAGCAACCCGACCCAGACCAGGGCGCTTGATGACATCAAGTTTCATACGAACCTGGTGGTCGAGCCGA
TCCTGGTGGACGAGGACCAGATCCGTCGCACCTTGGAGCAATGGCAAGCTAGCAACGCATCGCTTGGTTCATCGCTGGGG
GGCGATGACGAAGAGATGGGCGACCTGGACGTCTCGGCCGGCGACGAAGACATGGGCGCCGGCGGGGATGCCGGGGTCGA
TGCCAAGGGTGATGACACGCCGGTGGTGAAATTCGTCAACAAGGTGTTGGTGGATGCGATCCGGCGGGGAGCCTCCGATA
TCCATTTCGAGCCTTATGAAGACGACTACCGTGTTCGCTTGCGCATCGATGGGCTGCTGAAGAACGTGGCCAAGGCGCCG
GTGAAGCTGAGCCAGCGGATTGCGGCGCGGTTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGA
TGGGCGCATCAAGCTTAATCTGTCCAAGACCAAACAGATCGACTTCCGTGTGAGTACCTTGCCGACGCTGTTCGGCGAGA
AGGTGGTGCTACGTATCCTGGATGGCAGTGCGGCCAAGCTGGGGATCGAGAAGCTAGGTTACGAGGCGGACCAGCAGAAG
CTGTTCCTGGATGCGATCCACAAGCCCTACGGGATGGTGCTGGTGACCGGGCCGACCGGTTCGGGCAAGACGGTGTCCTT
GTATACGGCGCTGGGTATCCTCAACGATGAGACGCGCAACATCTCTACTGCCGAGGATCCGGTTGAAATTCGCCTGCCTG
GCGTCAATCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTTGCGGCGGCCTTGCGCTCGTTCCTGCGCCAGGACCCT
GACATCATCATGGTCGGCGAAATTCGCGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACCGGCCATATGGT
GCTGTCCACGTTGCATACCAACGATGCGCCACAGACCATCGCGCGCTTGATGAACATGGGGATTGCGCCGTACAACATCA
CCTCGTCGGTGACCTTGGTCATTGCGCAGCGCCTGGCGCGGCGGTTGTGCAACAACTGCAAGCGCAAGACCAGCTTGCCA
GAGCATGCGTTGCTGGCCGAAGGGTTTACGCAACAGCAGATCGACCAGGGCATTACGCTGTATGAGGCGGTCGGTTGCGA
TGACTGCACCGAGGGCTACAAGGGCCGTACGGGTATCTACCAGGTCATGCCGATGACCGACGAGATCGGTGCGATCGTGC
TGGAAGGCGGCAATGCGATGCAGATCGCCGAGGCGGCGCAGAAGATCGGTATCCGCGACTTGCGGCAGTCGGCCTTGATG
AAGGCCGCGCATGGGGTGACCAGCCTGGCGGAGATCAATCGGGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-153)

Type II/IV secretion system protein (T2SSE)

(201-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.162

98.27

0.552
  pilB Acinetobacter baylyi ADP1

55.458

98.27

0.545
  pilB Legionella pneumophila strain ERS1305867

52.373

98.443

0.516
  pilB Vibrio cholerae strain A1552

49.04

99.135

0.486
  pilF Neisseria gonorrhoeae MS11

48.592

98.27

0.478
  pilB Vibrio campbellii strain DS40M4

46.127

98.27

0.453
  pilB Vibrio parahaemolyticus RIMD 2210633

45.922

97.578

0.448
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.002

93.599

0.365