Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CFSAN002069_RS18385 Genome accession   NC_021812
Coordinates   3683752..3684732 (-) Length   326 a.a.
NCBI ID   WP_001055652.1    Uniprot ID   A0A5U3JPW7
Organism   Salmonella enterica subsp. enterica serovar Heidelberg str. CFSAN002069     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3678752..3689732
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CFSAN002069_RS18355 (CFSAN002069_16690) endA 3679209..3679916 (+) 708 WP_000286124.1 deoxyribonuclease I -
  CFSAN002069_RS18360 (CFSAN002069_16685) rsmE 3679993..3680724 (+) 732 WP_001222488.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  CFSAN002069_RS18365 (CFSAN002069_16680) gshB 3680744..3681691 (+) 948 WP_000593248.1 glutathione synthase -
  CFSAN002069_RS18370 (CFSAN002069_16675) - 3681907..3682470 (+) 564 WP_001053167.1 YqgE/AlgH family protein -
  CFSAN002069_RS18375 (CFSAN002069_16670) ruvX 3682470..3682886 (+) 417 WP_001285491.1 Holliday junction resolvase RuvX -
  CFSAN002069_RS18380 (CFSAN002069_16665) - 3682933..3683619 (-) 687 WP_000098325.1 IclR family transcriptional regulator -
  CFSAN002069_RS18385 (CFSAN002069_16660) pilT 3683752..3684732 (-) 981 WP_001055652.1 type IV pilus twitching motility protein PilT Machinery gene
  CFSAN002069_RS18390 (CFSAN002069_16655) - 3684750..3685454 (+) 705 WP_000997787.1 YggS family pyridoxal phosphate-dependent enzyme -
  CFSAN002069_RS18395 (CFSAN002069_16650) - 3685473..3686039 (+) 567 WP_001094848.1 YggT family protein -
  CFSAN002069_RS18400 (CFSAN002069_16645) yggU 3686036..3686326 (+) 291 WP_001277204.1 DUF167 family protein YggU -
  CFSAN002069_RS18405 (CFSAN002069_16640) - 3686334..3686927 (+) 594 WP_001174769.1 XTP/dITP diphosphatase -
  CFSAN002069_RS18410 (CFSAN002069_16635) hemW 3686920..3688056 (+) 1137 WP_001096521.1 radical SAM family heme chaperone HemW -
  CFSAN002069_RS18415 (CFSAN002069_16630) - 3688148..3689155 (-) 1008 WP_000252197.1 DUF1202 domain-containing protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36167.41 Da        Isoelectric Point: 7.8163

>NTDB_id=59995 CFSAN002069_RS18385 WP_001055652.1 3683752..3684732(-) (pilT) [Salmonella enterica subsp. enterica serovar Heidelberg str. CFSAN002069]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMETAPFTAPDVDRLLLDWLNDAQQYQWRKHGQLDFAVSLSGTRRLRA
SAFTHQQGTSLALRLLPERCPDLAEIQTPPIVPALLASENGLILVTGATGCGKSTTLAAMVGHLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QVQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=59995 CFSAN002069_RS18385 WP_001055652.1 3683752..3684732(-) (pilT) [Salmonella enterica subsp. enterica serovar Heidelberg str. CFSAN002069]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGACCGCGCCTTTTACCGCGCCTGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG
ACGCGCAACAATATCAGTGGCGAAAGCACGGCCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTGCGCGCC
AGTGCGTTCACACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGCGTTGTCCTGATCTGGCGGAAATCCA
GACGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCCACTGGCTGCGGGAAAT
CCACTACGCTGGCGGCGATGGTGGGACATCTTAATCAACATGCCGATAAACACATCCTGACGCTGGAAGATCCTATTGAG
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCGGCGTTACGTGAAGATCCCGATGTCATTTTACTTGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGTGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGGTACGCAGCCAACTGGCGGGTAGTCTGCGCGCCGTACTATCACAAAAACTGGAGGT
CGACAGACAGGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGTGCAGGGACGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(76-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5U3JPW7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.318

100

0.485
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.318

100

0.485
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Acinetobacter baylyi ADP1

44.954

100

0.451
  pilT Acinetobacter baumannii strain A118

44.648

100

0.448
  pilT Acinetobacter baumannii D1279779

44.648

100

0.448
  pilT Acinetobacter nosocomialis M2

45.455

97.853

0.445
  pilT Pseudomonas aeruginosa PAK

45.886

96.933

0.445
  pilT Pseudomonas stutzeri DSM 10701

45.886

96.933

0.445
  pilT Legionella pneumophila strain ERS1305867

44.654

97.546

0.436
  pilT Legionella pneumophila strain Lp02

44.654

97.546

0.436
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

38.558

97.853

0.377
  pilU Pseudomonas stutzeri DSM 10701

35.952

100

0.365

Multiple sequence alignment