Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   K4A87_RS04850 Genome accession   NZ_CP082178
Coordinates   1034683..1035090 (+) Length   135 a.a.
NCBI ID   WP_208589544.1    Uniprot ID   -
Organism   Xanthomonas fragariae strain SHQP01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1029683..1040090
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K4A87_RS04830 (K4A87_04785) coaE 1031057..1031689 (-) 633 WP_208589532.1 dephospho-CoA kinase -
  K4A87_RS04835 (K4A87_04790) - 1031703..1032566 (-) 864 WP_208589534.1 A24 family peptidase -
  K4A87_RS04840 (K4A87_04795) pilC 1032574..1033830 (-) 1257 WP_208591561.1 type II secretion system F family protein Machinery gene
  K4A87_RS04845 (K4A87_04800) pilA 1034198..1034608 (+) 411 WP_208589542.1 pilin Machinery gene
  K4A87_RS04850 (K4A87_04805) pilA/pilAI 1034683..1035090 (+) 408 WP_208589544.1 pilin Machinery gene
  K4A87_RS04855 (K4A87_04810) pilB 1035207..1036943 (+) 1737 WP_208589545.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K4A87_RS04860 (K4A87_04815) - 1036990..1037586 (-) 597 WP_208589553.1 Fic family protein -
  K4A87_RS04865 (K4A87_04820) - 1037583..1037765 (-) 183 WP_208589555.1 YhfG family protein -
  K4A87_RS04870 (K4A87_04825) pilR 1037840..1039288 (-) 1449 WP_208591562.1 sigma-54 dependent transcriptional regulator Regulator

Sequence

Protein


Download         Length: 135 a.a.        Molecular weight: 13987.11 Da        Isoelectric Point: 8.1079

>NTDB_id=599575 K4A87_RS04850 WP_208589544.1 1034683..1035090(+) (pilA/pilAI) [Xanthomonas fragariae strain SHQP01]
MSGQKGFSLIELMIVIAIIAILAAIAFPAYQSSVAKAQLTAALAEIRPAKTTLETVVQDGRDPSLVDKSYVGLTASTRCS
SVSATLDSSGVGEISCTLQGSSLVSGRDLKLKRSAAGLWACDASAFDQKYRPAGC

Nucleotide


Download         Length: 408 bp        

>NTDB_id=599575 K4A87_RS04850 WP_208589544.1 1034683..1035090(+) (pilA/pilAI) [Xanthomonas fragariae strain SHQP01]
ATGTCTGGGCAGAAAGGCTTTTCTCTAATAGAGTTGATGATTGTCATAGCGATTATTGCCATATTAGCTGCTATAGCATT
TCCGGCTTATCAAAGTTCCGTGGCAAAAGCGCAGCTTACCGCTGCATTGGCCGAAATAAGGCCAGCGAAAACAACGCTTG
AAACGGTGGTGCAGGATGGCAGGGATCCATCGCTCGTTGATAAAAGTTACGTGGGTTTGACAGCATCTACCAGGTGTTCC
AGCGTCAGTGCGACTCTGGATTCCTCCGGGGTTGGTGAAATTAGTTGTACTCTTCAAGGGTCTTCCCTGGTTTCAGGACG
TGATTTAAAGTTGAAGCGTTCTGCTGCTGGTCTATGGGCCTGTGACGCCTCTGCTTTTGACCAAAAATATCGCCCTGCTG
GTTGTTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-135)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

47.794

100

0.481
  pilA Acinetobacter baumannii strain A118

43.885

100

0.452
  pilA/pilAII Pseudomonas stutzeri DSM 10701

44.444

100

0.444
  comP Acinetobacter baylyi ADP1

38.776

100

0.422
  pilA Pseudomonas aeruginosa PAK

38.255

100

0.422
  pilA Ralstonia pseudosolanacearum GMI1000

33.333

100

0.4
  pilE Neisseria gonorrhoeae MS11

32.121

100

0.393
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36.364

100

0.385
  pilA Vibrio cholerae C6706

36.364

100

0.385
  pilA Vibrio cholerae strain A1552

36.364

100

0.385
  pilA2 Legionella pneumophila str. Paris

37.5

100

0.378
  pilA2 Legionella pneumophila strain ERS1305867

36.765

100

0.37