Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   K6T22_RS11930 Genome accession   NZ_CP081878
Coordinates   2251967..2253013 (-) Length   348 a.a.
NCBI ID   WP_238237434.1    Uniprot ID   -
Organism   Exiguobacterium acetylicum strain UTDF19-27C     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2246967..2258013
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6T22_RS11910 (K6T22_11900) - 2247527..2249266 (-) 1740 WP_238237428.1 fimbrial assembly protein -
  K6T22_RS11915 (K6T22_11905) - 2249285..2250040 (-) 756 WP_238237430.1 A24 family peptidase -
  K6T22_RS11920 (K6T22_11910) - 2250097..2250606 (-) 510 WP_238237431.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  K6T22_RS11925 (K6T22_11915) - 2250745..2251962 (-) 1218 WP_238237433.1 type II secretion system F family protein -
  K6T22_RS11930 (K6T22_11920) pilT 2251967..2253013 (-) 1047 WP_238237434.1 type IV pilus twitching motility protein PilT Machinery gene
  K6T22_RS11935 (K6T22_11925) - 2253010..2254674 (-) 1665 WP_238237436.1 ATPase, T2SS/T4P/T4SS family -
  K6T22_RS11940 (K6T22_11930) - 2254683..2254997 (-) 315 WP_238237437.1 hypothetical protein -
  K6T22_RS11945 (K6T22_11935) - 2255014..2256372 (-) 1359 WP_238237441.1 G5 domain-containing protein -
  K6T22_RS11950 (K6T22_11940) - 2256532..2257026 (+) 495 WP_238237442.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  K6T22_RS11955 (K6T22_11945) - 2256980..2257567 (+) 588 WP_238237444.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 38218.94 Da        Isoelectric Point: 7.1317

>NTDB_id=598012 K6T22_RS11930 WP_238237434.1 2251967..2253013(-) (pilT) [Exiguobacterium acetylicum strain UTDF19-27C]
MNSTIERAQIEDILRSSKERDASDVHITAGSPPVFRINGTLAPFGTEKMKPVIIDGFVRSLITDEMYQQLKQDRDIDFSF
GVPGVARYRVNCFYQRGALSMAFRTIPTEVPTLEQLALPPVLKRFLTKPHGLILVTGPTGSGKSTTLAAMINEINQTMYK
RIITLEDPIEYLHSHHKSLIDQREIGIDAPRFATGLRSALRQDPDVILLGEMRDLETIATAVTAAETGHLVFATVHTSTA
ASTVSRIIDVFPSEQQDQIRAQLANVLAGVISQRLMPRTDGTGRVAALEIMVDTPAVSNLIRTGKEYQIQSVLQTGKQYG
MQTMQMALQDLVSRGLIPASAATPYISG

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=598012 K6T22_RS11930 WP_238237434.1 2251967..2253013(-) (pilT) [Exiguobacterium acetylicum strain UTDF19-27C]
ATGAACAGTACGATTGAACGTGCGCAAATCGAGGACATATTACGTTCCTCCAAAGAACGAGATGCATCCGATGTCCATAT
TACGGCGGGGTCGCCACCTGTTTTTCGAATCAATGGAACGTTAGCCCCGTTTGGTACGGAAAAAATGAAGCCCGTCATCA
TTGATGGATTCGTTCGCTCCTTGATTACAGACGAGATGTATCAACAGTTAAAGCAAGATCGGGACATTGATTTTTCGTTT
GGAGTACCGGGCGTCGCTCGGTACCGGGTCAACTGTTTTTATCAACGGGGCGCATTATCGATGGCTTTTCGAACGATCCC
GACGGAAGTGCCGACGCTTGAACAATTAGCACTGCCTCCTGTTCTGAAACGTTTTTTGACGAAACCGCACGGCTTGATTC
TTGTGACTGGTCCAACCGGATCTGGAAAGTCAACGACGCTCGCAGCGATGATCAATGAAATCAATCAGACGATGTATAAA
CGAATCATCACACTTGAAGATCCGATCGAATACTTACATAGTCACCATAAAAGTCTGATTGATCAGCGGGAAATTGGAAT
CGATGCGCCACGCTTCGCAACCGGATTACGGTCGGCACTTCGTCAGGATCCGGATGTCATCTTGCTCGGTGAGATGCGAG
ATCTTGAGACGATTGCAACTGCCGTTACGGCTGCTGAAACCGGTCACCTCGTCTTCGCAACGGTCCATACGTCGACGGCT
GCCTCAACGGTTAGTCGGATCATTGATGTTTTCCCGTCAGAGCAACAAGACCAGATTCGGGCTCAGCTAGCGAACGTCCT
GGCTGGCGTTATCTCGCAACGACTGATGCCCCGTACGGACGGAACAGGGCGTGTCGCGGCACTTGAAATCATGGTCGATA
CACCAGCCGTTTCAAACTTAATTCGGACCGGTAAGGAATATCAGATTCAATCTGTCTTACAGACAGGTAAACAATATGGC
ATGCAGACGATGCAAATGGCGTTGCAGGACCTCGTTAGCCGAGGATTGATTCCAGCATCTGCTGCAACGCCGTACATATC
GGGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

50.599

95.977

0.486
  pilT Neisseria gonorrhoeae MS11

50.599

95.977

0.486
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.61

94.253

0.477
  pilT Acinetobacter nosocomialis M2

49.552

96.264

0.477
  pilT Acinetobacter baumannii D1279779

49.254

96.264

0.474
  pilT Acinetobacter baumannii strain A118

49.254

96.264

0.474
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.697

94.828

0.471
  pilT Vibrio cholerae strain A1552

49.697

94.828

0.471
  pilT Pseudomonas aeruginosa PAK

49.249

95.69

0.471
  pilT Acinetobacter baylyi ADP1

49.085

94.253

0.463
  pilT Pseudomonas stutzeri DSM 10701

48.048

95.69

0.46
  pilT Legionella pneumophila strain Lp02

46.547

95.69

0.445
  pilT Legionella pneumophila strain ERS1305867

46.547

95.69

0.445
  pilU Acinetobacter baylyi ADP1

40.299

96.264

0.388
  pilU Pseudomonas stutzeri DSM 10701

39.818

94.54

0.376