Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   K4H25_RS04570 Genome accession   NZ_CP081149
Coordinates   967105..968241 (+) Length   378 a.a.
NCBI ID   WP_221022203.1    Uniprot ID   -
Organism   Deefgea piscis strain D13     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 931135..1017419 967105..968241 within 0


Gene organization within MGE regions


Location: 931135..1017419
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K4H25_RS04420 (K4H25_04420) - 931965..932555 (+) 591 WP_255588018.1 HD-GYP domain-containing protein -
  K4H25_RS04425 (K4H25_04425) - 932651..934360 (-) 1710 WP_221022178.1 chitinase -
  K4H25_RS04430 (K4H25_04430) metF 934596..935432 (+) 837 WP_221022179.1 methylenetetrahydrofolate reductase [NAD(P)H] -
  K4H25_RS04435 (K4H25_04435) - 935532..936002 (-) 471 WP_221022180.1 glycine zipper 2TM domain-containing protein -
  K4H25_RS04440 (K4H25_04440) - 936103..936687 (-) 585 WP_221022181.1 2OG-Fe(II) oxygenase -
  K4H25_RS04445 (K4H25_04445) - 936684..938702 (-) 2019 WP_221022182.1 site-specific recombinase -
  K4H25_RS04450 (K4H25_04450) - 939006..941813 (+) 2808 WP_221022183.1 pitrilysin family protein -
  K4H25_RS04455 (K4H25_04455) - 941957..942955 (-) 999 WP_221022184.1 acyltransferase -
  K4H25_RS04460 (K4H25_04460) - 943281..943556 (-) 276 WP_221022185.1 YkgJ family cysteine cluster protein -
  K4H25_RS04465 (K4H25_04465) - 943688..947506 (-) 3819 WP_221022186.1 phospholipid carrier-dependent glycosyltransferase -
  K4H25_RS04470 (K4H25_04470) - 947993..948937 (+) 945 WP_173533247.1 glycosyltransferase family 2 protein -
  K4H25_RS04475 (K4H25_04475) - 949043..949447 (+) 405 WP_221022187.1 GtrA family protein -
  K4H25_RS04480 (K4H25_04480) - 949819..950217 (-) 399 WP_221022188.1 YidB family protein -
  K4H25_RS04485 (K4H25_04485) - 950353..952428 (-) 2076 WP_221022189.1 bifunctional diguanylate cyclase/phosphodiesterase -
  K4H25_RS04490 (K4H25_04490) - 952415..953362 (-) 948 WP_221022190.1 HDOD domain-containing protein -
  K4H25_RS04495 (K4H25_04495) - 953711..954454 (+) 744 WP_221022191.1 LOG family protein -
  K4H25_RS04500 (K4H25_04500) hemG 954472..955014 (-) 543 WP_221022192.1 menaquinone-dependent protoporphyrinogen IX dehydrogenase -
  K4H25_RS04505 (K4H25_04505) - 955658..957109 (-) 1452 WP_221022193.1 S41 family peptidase -
  K4H25_RS04510 (K4H25_04510) - 957160..958527 (-) 1368 WP_221022194.1 murein hydrolase activator EnvC -
  K4H25_RS04515 (K4H25_04515) gpmI 958649..960181 (-) 1533 WP_221022195.1 2,3-bisphosphoglycerate-independent phosphoglycerate mutase -
  K4H25_RS04520 (K4H25_04520) - 960374..960679 (+) 306 WP_226763265.1 helix-turn-helix transcriptional regulator -
  K4H25_RS04525 (K4H25_04525) - 960742..961206 (-) 465 WP_221022196.1 TlpA disulfide reductase family protein -
  K4H25_RS04530 (K4H25_04530) dsbD 961203..962753 (-) 1551 WP_221022197.1 protein-disulfide reductase DsbD -
  K4H25_RS04535 (K4H25_04535) cutA 962754..963089 (-) 336 WP_221022198.1 divalent-cation tolerance protein CutA -
  K4H25_RS04540 (K4H25_04540) - 963088..963501 (+) 414 WP_221022199.1 FxsA family protein -
  K4H25_RS17105 (K4H25_04545) - 963503..963796 (-) 294 WP_173533261.1 DUF167 domain-containing protein -
  K4H25_RS04550 (K4H25_04550) - 963796..964383 (-) 588 WP_221022200.1 YggT family protein -
  K4H25_RS04555 (K4H25_04555) proC 964400..965215 (-) 816 WP_221022201.1 pyrroline-5-carboxylate reductase -
  K4H25_RS04560 (K4H25_04560) - 965265..965963 (-) 699 WP_374706354.1 YggS family pyridoxal phosphate-dependent enzyme -
  K4H25_RS04565 (K4H25_04565) pilT 966049..967092 (+) 1044 WP_173533265.1 type IV pilus twitching motility protein PilT Machinery gene
  K4H25_RS04570 (K4H25_04570) pilU 967105..968241 (+) 1137 WP_221022203.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K4H25_RS04575 (K4H25_04575) - 968623..970674 (+) 2052 WP_221022204.1 FimV family protein -
  K4H25_RS04580 (K4H25_04580) uvrA 970741..973596 (-) 2856 WP_221022205.1 excinuclease ABC subunit UvrA -
  K4H25_RS04585 (K4H25_04585) - 973717..974790 (-) 1074 WP_221022206.1 GGDEF domain-containing protein -
  K4H25_RS04590 (K4H25_04590) - 974941..975891 (+) 951 WP_255588190.1 phosphotransferase -
  K4H25_RS04595 (K4H25_04595) - 976193..977587 (+) 1395 WP_255588030.1 MFS transporter -
  K4H25_RS04600 (K4H25_04600) ssb 977647..978162 (+) 516 WP_221022208.1 single-stranded DNA-binding protein Machinery gene
  K4H25_RS04605 (K4H25_04605) - 978353..980560 (-) 2208 WP_221022209.1 OsmC domain/YcaO domain-containing protein -
  K4H25_RS04610 (K4H25_04610) - 980945..981994 (+) 1050 WP_221022210.1 sensor domain-containing diguanylate cyclase -
  K4H25_RS04615 (K4H25_04615) hmpA 982120..983316 (+) 1197 WP_221022211.1 NO-inducible flavohemoprotein -
  K4H25_RS04620 (K4H25_04620) - 983488..985263 (-) 1776 WP_221022212.1 sigma-54-dependent Fis family transcriptional regulator -
  K4H25_RS04625 (K4H25_04625) adh 985652..987172 (+) 1521 WP_221022213.1 aldehyde dehydrogenase -
  K4H25_RS04630 (K4H25_04630) - 987246..987644 (+) 399 WP_221022214.1 DUF779 domain-containing protein -
  K4H25_RS04635 (K4H25_04635) - 987719..989173 (-) 1455 WP_255588035.1 NAD-dependent succinate-semialdehyde dehydrogenase -
  K4H25_RS04640 (K4H25_04640) gabT 989160..990446 (-) 1287 WP_221022215.1 4-aminobutyrate--2-oxoglutarate transaminase -
  K4H25_RS04645 (K4H25_04645) - 990581..992005 (-) 1425 WP_221022216.1 gamma-aminobutyraldehyde dehydrogenase -
  K4H25_RS04650 (K4H25_04650) - 992047..992880 (-) 834 WP_221022217.1 ABC transporter permease -
  K4H25_RS04655 (K4H25_04655) - 992870..993811 (-) 942 WP_255588041.1 ABC transporter permease -
  K4H25_RS04660 (K4H25_04660) - 993808..994815 (-) 1008 WP_221022218.1 ABC transporter ATP-binding protein -
  K4H25_RS04665 (K4H25_04665) - 994940..996088 (-) 1149 WP_221022219.1 ABC transporter substrate-binding protein -
  K4H25_RS04670 (K4H25_04670) - 996668..997606 (-) 939 WP_221022220.1 LysR family transcriptional regulator -
  K4H25_RS04675 (K4H25_04675) hdeA 998124..998456 (-) 333 WP_221022221.1 acid-activated periplasmic chaperone HdeA -
  K4H25_RS04680 (K4H25_04680) - 999040..999477 (+) 438 WP_221022222.1 DUF4826 family protein -
  K4H25_RS04685 (K4H25_04685) - 999530..1000072 (+) 543 WP_221022223.1 CbrC family protein -
  K4H25_RS04690 (K4H25_04690) phnF 1000473..1001183 (+) 711 WP_221022224.1 phosphonate metabolism transcriptional regulator PhnF -
  K4H25_RS04695 (K4H25_04695) - 1001187..1001531 (-) 345 WP_221022225.1 hypothetical protein -
  K4H25_RS04700 (K4H25_04700) - 1001737..1003140 (-) 1404 WP_221022226.1 FAD-dependent oxidoreductase -
  K4H25_RS04705 (K4H25_04705) phnX 1003137..1003967 (-) 831 WP_221022227.1 phosphonoacetaldehyde hydrolase -
  K4H25_RS04710 (K4H25_04710) - 1003960..1004913 (-) 954 WP_255588191.1 2-aminoethylphosphonate--pyruvate transaminase -
  K4H25_RS04715 (K4H25_04715) - 1004929..1005087 (-) 159 WP_221022229.1 hypothetical protein -
  K4H25_RS04720 (K4H25_04720) - 1005439..1007133 (-) 1695 WP_221022230.1 putative 2-aminoethylphosphonate ABC transporter permease subunit -
  K4H25_RS04725 (K4H25_04725) - 1007130..1008230 (-) 1101 WP_221022231.1 putative 2-aminoethylphosphonate ABC transporter ATP-binding protein -
  K4H25_RS04730 (K4H25_04730) - 1008505..1009524 (-) 1020 WP_221022232.1 putative 2-aminoethylphosphonate ABC transporter substrate-binding protein -
  K4H25_RS04735 (K4H25_04735) - 1009754..1010212 (+) 459 WP_221022233.1 Rrf2 family transcriptional regulator -
  K4H25_RS04740 (K4H25_04740) - 1010230..1010922 (+) 693 WP_221022234.1 DUF1345 domain-containing protein -
  K4H25_RS04745 (K4H25_04745) - 1011107..1012324 (+) 1218 WP_221022235.1 HD-GYP domain-containing protein -
  K4H25_RS04750 (K4H25_04750) eco 1012387..1012860 (-) 474 WP_221022236.1 serine protease inhibitor ecotin -
  K4H25_RS04755 (K4H25_04755) - 1013047..1013955 (-) 909 WP_221022237.1 PP2C family serine/threonine-protein phosphatase -
  K4H25_RS04760 (K4H25_04760) - 1013952..1014920 (-) 969 WP_221022238.1 serine/threonine-protein kinase -
  K4H25_RS04765 (K4H25_04765) - 1015097..1015951 (+) 855 WP_255588045.1 YicC/YloC family endoribonuclease -
  K4H25_RS04770 (K4H25_04770) - 1016145..1017419 (+) 1275 WP_221022240.1 site-specific integrase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42538.73 Da        Isoelectric Point: 6.5072

>NTDB_id=595359 K4H25_RS04570 WP_221022203.1 967105..968241(+) (pilU) [Deefgea piscis strain D13]
MEKEQAAKFMHDLLRHMRSKNASDLFITVDFPPAMKIDGRVTPVSNQQLTAQHTKELARAIMNDRQAEDFEANKECNFAI
SPGSMGRFRVNAFMQQGRVGMVLRTINSEIPKLDQLNLPPVLRDIAMTKRGLVIFVGGTGSGKSTSLAAMIGHRNENAYD
HIITIEDPIEYVHEHKNSIITQREVGVDTDSWMAALKNTLRQAPDVILIGEVRDRETMDYAIAFAETGHLCMATLHANSA
NQALDRIINFFPEERRSQLLMDLSLNLKAFVSQRLVPHLSGKGRVAAVEVMLNSPLISELIFKGEVHEIKEIMKKSRELG
MQTFDQSLFDLFEANRISYEDALRNADSVNDLRLQIKLNGTESKNRDVLSGLDHLDIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=595359 K4H25_RS04570 WP_221022203.1 967105..968241(+) (pilU) [Deefgea piscis strain D13]
ATGGAAAAAGAACAAGCCGCTAAGTTTATGCATGATTTATTGCGCCATATGCGCAGTAAAAATGCTTCGGATTTATTTAT
TACCGTTGATTTTCCGCCGGCAATGAAAATTGACGGTCGAGTGACTCCAGTTTCTAATCAGCAACTGACCGCACAACACA
CCAAAGAATTAGCTCGGGCGATTATGAATGATCGTCAAGCCGAAGACTTTGAAGCCAATAAAGAATGTAATTTCGCGATT
AGCCCTGGATCAATGGGGCGCTTTCGGGTGAATGCATTTATGCAGCAAGGCCGTGTGGGGATGGTGCTGCGAACGATTAA
TTCGGAAATCCCCAAGCTGGATCAATTAAATTTGCCGCCAGTATTGCGTGATATTGCGATGACTAAGCGCGGTTTAGTGA
TTTTTGTCGGTGGGACTGGCTCAGGTAAATCAACATCGTTAGCGGCAATGATTGGTCATCGGAATGAAAACGCCTATGAC
CATATCATTACCATTGAAGACCCGATTGAATACGTTCATGAGCATAAAAATTCGATTATTACGCAGCGAGAAGTGGGCGT
TGATACCGATTCTTGGATGGCTGCGCTTAAAAACACGCTGCGCCAAGCGCCCGATGTGATTTTGATCGGCGAAGTACGTG
ATCGTGAAACCATGGATTATGCGATTGCGTTTGCCGAAACCGGCCATCTTTGCATGGCAACATTGCATGCCAACTCGGCT
AATCAAGCACTGGATCGGATTATTAACTTTTTCCCTGAAGAACGCCGTTCCCAGTTGTTGATGGATTTATCGCTCAATTT
AAAAGCTTTTGTTTCGCAACGCTTAGTGCCGCACTTATCGGGTAAAGGCCGCGTTGCCGCAGTTGAAGTCATGCTCAATT
CACCATTGATTTCGGAGTTAATCTTTAAAGGTGAAGTTCACGAGATTAAAGAAATTATGAAAAAATCGCGTGAATTAGGC
ATGCAAACATTTGATCAGTCGCTATTTGATTTATTTGAAGCCAATCGCATTAGCTATGAAGATGCCTTACGTAATGCCGA
CTCGGTGAATGATTTACGCTTGCAAATCAAATTAAATGGCACAGAATCGAAAAATCGTGACGTTTTGTCTGGTCTAGATC
ATCTTGATATTGTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(44-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.291

94.709

0.59
  pilU Acinetobacter baylyi ADP1

57.923

96.825

0.561
  pilU Vibrio cholerae strain A1552

54.023

92.063

0.497
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.97

88.624

0.407
  pilT Acinetobacter baylyi ADP1

42.73

89.153

0.381
  pilT Pseudomonas stutzeri DSM 10701

42.09

88.624

0.373
  pilT Acinetobacter nosocomialis M2

42.598

87.566

0.373
  pilT Legionella pneumophila strain Lp02

42.598

87.566

0.373
  pilT Legionella pneumophila strain ERS1305867

42.598

87.566

0.373
  pilT Acinetobacter baumannii D1279779

42.598

87.566

0.373
  pilT Acinetobacter baumannii strain A118

42.598

87.566

0.373
  pilT Pseudomonas aeruginosa PAK

41.493

88.624

0.368
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.354

83.598

0.362
  pilT Vibrio cholerae strain A1552

43.354

83.598

0.362