Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   K0B96_RS08095 Genome accession   NZ_CP080507
Coordinates   1938916..1939998 (+) Length   360 a.a.
NCBI ID   WP_220165916.1    Uniprot ID   -
Organism   Horticoccus luteus strain KSB-15     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1933916..1944998
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K0B96_RS08080 (K0B96_08080) coaE 1934398..1934982 (+) 585 WP_220165912.1 dephospho-CoA kinase -
  K0B96_RS08085 (K0B96_08085) rho 1935021..1937072 (+) 2052 WP_255558900.1 transcription termination factor Rho -
  K0B96_RS08090 (K0B96_08090) - 1937086..1938816 (+) 1731 WP_220165913.1 ATPase, T2SS/T4P/T4SS family -
  K0B96_RS08095 (K0B96_08095) pilT 1938916..1939998 (+) 1083 WP_220165916.1 type IV pilus twitching motility protein PilT Machinery gene
  K0B96_RS08100 (K0B96_08100) gspE 1940008..1941699 (+) 1692 WP_220165918.1 type II secretion system ATPase GspE -
  K0B96_RS08105 (K0B96_08105) - 1941701..1942987 (+) 1287 WP_220165920.1 type II secretion system F family protein -
  K0B96_RS08110 (K0B96_08110) - 1943091..1944140 (-) 1050 WP_220165922.1 HD domain-containing protein -
  K0B96_RS08115 (K0B96_08115) - 1944133..1944687 (-) 555 WP_220165924.1 isochorismatase family protein -

Sequence

Protein


Download         Length: 360 a.a.        Molecular weight: 39662.83 Da        Isoelectric Point: 7.0212

>NTDB_id=593277 K0B96_RS08095 WP_220165916.1 1938916..1939998(+) (pilT) [Horticoccus luteus strain KSB-15]
MSYEMNDLLDLMVEQNASDLHIQVGQAPVLRLSGSMTPIDGPILGPADTERLMLSITPDAHISNVKLNGGADFGFAFGEK
ARFRVSILRAKGNYGMVLRQIPNRMFGLRDIGMPDKIRELLYRPRGLILVTGPTGSGKSTTLASLINYINENRDGHIITI
EDPIEYYHPHKRCLVTQREVHVDVPSFAEAIRRALRQDPDIILVGEMRDLETIEAAISAAETGHLVFGTLHTNSAAKTVD
RIVDAFPANMKEMIRTQLASSLQAVISQVLCKKTGGGRIAAYEIMINTTSIASLIRENKTFRIPSDIQTGANLGMITMDT
HLLSLHNRNLVSPDECVEKAQDPIVMRDKLTSLGAQLKVL

Nucleotide


Download         Length: 1083 bp        

>NTDB_id=593277 K0B96_RS08095 WP_220165916.1 1938916..1939998(+) (pilT) [Horticoccus luteus strain KSB-15]
ATGAGCTATGAGATGAACGACCTGCTCGACCTGATGGTGGAGCAAAACGCGTCTGACCTGCACATCCAAGTCGGACAAGC
TCCCGTCTTGCGCTTGAGCGGCAGCATGACGCCGATCGACGGTCCGATTCTGGGGCCGGCCGATACGGAGCGGCTGATGC
TTTCCATCACACCGGACGCACACATCAGCAACGTGAAGCTGAATGGCGGCGCGGACTTTGGTTTCGCGTTTGGCGAAAAG
GCGCGGTTTCGCGTGAGCATCCTCCGGGCGAAGGGCAATTACGGGATGGTGCTCCGCCAGATTCCCAACCGCATGTTCGG
ACTGCGCGATATCGGCATGCCTGACAAAATCCGCGAGCTGCTCTACCGGCCGCGCGGACTGATTCTGGTCACCGGGCCGA
CGGGCTCCGGCAAGTCGACGACGCTCGCGTCGCTGATCAACTACATTAACGAAAACCGCGACGGGCACATCATCACGATC
GAAGACCCGATTGAGTATTATCATCCGCACAAACGCTGTCTGGTGACGCAGCGGGAAGTGCACGTGGACGTGCCAAGTTT
CGCGGAGGCGATTCGCCGCGCGCTGCGTCAGGATCCGGACATCATCCTCGTCGGTGAAATGCGCGACCTGGAGACGATCG
AGGCGGCGATCAGTGCGGCGGAGACGGGCCATTTGGTTTTCGGCACGCTGCACACGAACAGCGCCGCCAAAACCGTGGAC
CGCATCGTGGACGCATTTCCGGCAAACATGAAGGAGATGATCCGCACACAGCTCGCGTCGTCGTTGCAGGCCGTGATTTC
CCAGGTGCTTTGCAAGAAGACGGGCGGCGGCCGCATCGCGGCCTACGAGATCATGATCAACACGACGTCCATCGCGTCGC
TGATTCGCGAGAACAAGACGTTTCGCATTCCGTCGGACATCCAAACGGGCGCCAATCTCGGGATGATCACGATGGACACG
CATTTGCTCAGTCTGCACAACCGCAACCTCGTCAGCCCGGATGAATGCGTGGAAAAGGCCCAGGATCCGATCGTGATGCG
GGACAAGCTCACCAGCCTCGGCGCGCAGTTGAAAGTGTTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(56-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

51.765

94.444

0.489
  pilT Acinetobacter baumannii D1279779

51.765

94.444

0.489
  pilT Acinetobacter baumannii strain A118

51.765

94.444

0.489
  pilT Pseudomonas stutzeri DSM 10701

51.765

94.444

0.489
  pilT Pseudomonas aeruginosa PAK

50.882

94.444

0.481
  pilT Acinetobacter baylyi ADP1

50

94.444

0.472
  pilT Legionella pneumophila strain Lp02

50.148

93.611

0.469
  pilT Legionella pneumophila strain ERS1305867

50.148

93.611

0.469
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.267

94.722

0.467
  pilT Neisseria meningitidis 8013

48.824

94.444

0.461
  pilT Neisseria gonorrhoeae MS11

48.529

94.444

0.458
  pilT Vibrio cholerae strain A1552

47.337

93.889

0.444
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

47.337

93.889

0.444
  pilU Acinetobacter baylyi ADP1

39.884

96.111

0.383
  pilU Vibrio cholerae strain A1552

39.528

94.167

0.372