Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KY275_RS19815 Genome accession   NZ_CP079939
Coordinates   3753113..3754093 (-) Length   326 a.a.
NCBI ID   WP_213788987.1    Uniprot ID   -
Organism   Enterobacter hormaechei strain G17     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3748113..3759093
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KY275_RS19785 (KY275_19790) - 3748990..3749487 (+) 498 WP_017692693.1 SprT family zinc-dependent metalloprotease -
  KY275_RS19790 (KY275_19795) endA 3749582..3750289 (+) 708 WP_003860034.1 deoxyribonuclease I -
  KY275_RS19795 (KY275_19800) rsmE 3750341..3751072 (+) 732 WP_017382923.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  KY275_RS19800 (KY275_19805) gshB 3751104..3752051 (+) 948 WP_015571835.1 glutathione synthase -
  KY275_RS19805 (KY275_19810) - 3752126..3752686 (+) 561 WP_006811924.1 YqgE/AlgH family protein -
  KY275_RS19810 (KY275_19815) ruvX 3752686..3753102 (+) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  KY275_RS19815 (KY275_19820) pilT 3753113..3754093 (-) 981 WP_213788987.1 type IV pilus twitching motility protein PilT Machinery gene
  KY275_RS19820 (KY275_19825) - 3754111..3754812 (+) 702 WP_213788986.1 YggS family pyridoxal phosphate-dependent enzyme -
  KY275_RS19825 (KY275_19830) - 3754834..3755400 (+) 567 WP_015571838.1 YggT family protein -
  KY275_RS19830 (KY275_19835) yggU 3755397..3755693 (+) 297 WP_062933226.1 DUF167 family protein YggU -
  KY275_RS19835 (KY275_19840) - 3755697..3756290 (+) 594 WP_015571839.1 XTP/dITP diphosphatase -
  KY275_RS19840 (KY275_19845) hemW 3756283..3757431 (+) 1149 WP_213788985.1 radical SAM family heme chaperone HemW -
  KY275_RS19845 (KY275_19850) - 3757488..3757850 (+) 363 WP_213788984.1 endonuclease domain-containing protein -
  KY275_RS19850 (KY275_19855) - 3758056..3758772 (-) 717 WP_003862419.1 DUF2884 domain-containing protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35666.57 Da        Isoelectric Point: 5.8283

>NTDB_id=590330 KY275_RS19815 WP_213788987.1 3753113..3754093(-) (pilT) [Enterobacter hormaechei strain G17]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVEALLKAWLNDEQQGAWWANGQVDFAVTLADGQRLRG
SAFKHMHGVSIALRLLPLTCPQLSALGVPRAIPELLSNDNGLILVTGATGSGKSTTLAAMVDFLNHQTDGHILTLEDPVE
FMYESERCLIQQREIGQHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLRQDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=590330 KY275_RS19815 WP_213788987.1 3753113..3754093(-) (pilT) [Enterobacter hormaechei strain G17]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGGTCAGGCCGTCTTGAACCTGCGCCGTTTCCGCCCCCGGATGTGGAGGCATTATTAAAAGCGTGGCTCAACG
ATGAACAGCAGGGCGCATGGTGGGCAAACGGGCAGGTGGATTTTGCCGTTACCCTCGCAGACGGTCAGCGGCTGCGCGGC
AGTGCGTTTAAGCATATGCACGGCGTTTCGATTGCGCTGCGGCTGTTGCCGCTGACGTGCCCGCAGCTCTCTGCGTTAGG
TGTGCCGCGAGCGATCCCGGAACTGCTGTCTAATGACAATGGCCTGATTTTGGTCACCGGCGCCACCGGCAGCGGAAAAT
CGACCACCCTGGCCGCGATGGTCGATTTCCTCAATCATCAGACAGACGGGCATATTCTGACCCTGGAAGATCCGGTGGAG
TTTATGTACGAGAGCGAACGTTGCCTGATCCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCCTTTGCCGAGGCGCTACG
CAGCGCGCTGCGTGAAGATCCGGATGTGATTCTGCTTGGTGAGCTGCGCGACAGCGAGACGATACGCCTGGCGCTGACGG
CGGCGGAAACCGGCCATCTGGTGTTAGCCACGCTGCATACGCGCGGCGCGGCGCAGGCGATTGAGCGCCTGGTCGATACC
TTTCCGGCGCAGGAGAAAGATCCGGTGCGTAATCAGCTGGCGGGAAGCCTGCGTGCGGTCCTGGCGCAGAAGCTACGTCA
GGATGTCCAGGGCGGGCGCGTGGCGCTGTATGAGCTGCTGGTGAATACGTCGGCGGCGGCCAACCTGATCCGCGAAGGCA
AAACCTGGCAACTGCCGGGCATTATTCAAACGGGGCAGCAGGCGGGAATGCAGAACTTTGAGCAGAGTCTGGCGGAGCGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(72-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Pseudomonas stutzeri DSM 10701

48.012

100

0.482
  pilT Legionella pneumophila strain Lp02

48.447

98.773

0.479
  pilT Legionella pneumophila strain ERS1305867

48.447

98.773

0.479
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Pseudomonas aeruginosa PAK

47.095

100

0.472
  pilT Acinetobacter baylyi ADP1

47.095

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Acinetobacter baumannii strain A118

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

47.188

98.16

0.463
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.653

99.08

0.433
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilU Vibrio cholerae strain A1552

38.509

98.773

0.38