Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DAX60_RS03675 Genome accession   NZ_CP077702
Coordinates   766949..767929 (+) Length   326 a.a.
NCBI ID   WP_023226315.1    Uniprot ID   A0A8F6XI23
Organism   Salmonella enterica subsp. enterica strain CFSAN058588     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 761949..772929
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DAX60_RS03645 (DAX60_003645) - 762527..763534 (+) 1008 WP_001527908.1 DUF1202 domain-containing protein -
  DAX60_RS03650 (DAX60_003650) hemW 763625..764761 (-) 1137 WP_001527976.1 radical SAM family heme chaperone HemW -
  DAX60_RS03655 (DAX60_003655) - 764754..765347 (-) 594 WP_001528003.1 XTP/dITP diphosphatase -
  DAX60_RS03660 (DAX60_003660) yggU 765355..765645 (-) 291 WP_001277203.1 DUF167 family protein YggU -
  DAX60_RS03665 (DAX60_003665) - 765642..766208 (-) 567 WP_001094848.1 YggT family protein -
  DAX60_RS03670 (DAX60_003670) - 766227..766931 (-) 705 WP_000997790.1 YggS family pyridoxal phosphate-dependent enzyme -
  DAX60_RS03675 (DAX60_003675) pilT 766949..767929 (+) 981 WP_023226315.1 type IV pilus twitching motility protein PilT Machinery gene
  DAX60_RS03680 (DAX60_003680) - 768061..768747 (+) 687 WP_001527920.1 IclR family transcriptional regulator -
  DAX60_RS03685 (DAX60_003685) ruvX 768794..769210 (-) 417 WP_000017096.1 Holliday junction resolvase RuvX -
  DAX60_RS03690 (DAX60_003690) - 769210..769773 (-) 564 WP_001053171.1 YqgE/AlgH family protein -
  DAX60_RS03695 (DAX60_003695) gshB 769989..770936 (-) 948 WP_000593248.1 glutathione synthase -
  DAX60_RS03700 (DAX60_003700) rsmE 770956..771687 (-) 732 WP_001222488.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  DAX60_RS03705 (DAX60_003705) endA 771764..772471 (-) 708 WP_001527975.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36078.25 Da        Isoelectric Point: 7.5632

>NTDB_id=582390 DAX60_RS03675 WP_023226315.1 766949..767929(+) (pilT) [Salmonella enterica subsp. enterica strain CFSAN058588]
MNMEEIVTLSVKHNVSDLHLSNAWPARWRKQGRMEIAPFTAPDVDRLLLDWLNDAQQYQWRAHGQLDFAVSLSGTRRLRA
SAFTHQQGTSLALRLLPERCPDLAEIQTPPIVPALLASENGLILVTGATGCGKSTTLAAMVGHLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=582390 DAX60_RS03675 WP_023226315.1 766949..767929(+) (pilT) [Salmonella enterica subsp. enterica strain CFSAN058588]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGAGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGATCGCGCCTTTTACCGCGCCAGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG
ACGCGCAACAATATCAGTGGCGAGCGCACGGCCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTACGCGCC
AGTGCGTTCACACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGCGTTGTCCTGATCTGGCGGAAATCCA
GACGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCTACTGGCTGCGGGAAGT
CCACCACGCTGGCGGCGATGGTGGGACATCTTAATCAACATGCCGATAAACACATCCTGACGCTGGAAGATCCTATTGAG
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCGGCGTTGCGTGAAGATCCCGATGTCATTTTGCTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGTGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGGTACGCAGTCAACTGGCGGGTAGTCTGCGCGCTGTACTATCGCAAAAACTGGAGGT
CGACAGACAGGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGCGCAGGGACGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(75-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.93

100

0.491
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.93

100

0.491
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Acinetobacter baumannii strain A118

45.26

100

0.454
  pilT Acinetobacter baumannii D1279779

45.26

100

0.454
  pilT Acinetobacter baylyi ADP1

45.26

100

0.454
  pilT Pseudomonas aeruginosa PAK

45.26

100

0.454
  pilT Pseudomonas stutzeri DSM 10701

44.954

100

0.451
  pilT Acinetobacter nosocomialis M2

44.954

100

0.451
  pilT Legionella pneumophila strain Lp02

44.969

97.546

0.439
  pilT Legionella pneumophila strain ERS1305867

44.969

97.546

0.439
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

38.558

97.853

0.377
  pilU Pseudomonas stutzeri DSM 10701

36.336

100

0.371