Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HU754_RS25590 Genome accession   NZ_CP077090
Coordinates   5803678..5804109 (-) Length   143 a.a.
NCBI ID   WP_093434881.1    Uniprot ID   -
Organism   Pseudomonas zeae strain OE 48.2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5798678..5809109
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HU754_RS25575 (HU754_025575) - 5799512..5802460 (+) 2949 WP_186625122.1 DUF748 domain-containing protein -
  HU754_RS25580 (HU754_025580) - 5802460..5802756 (+) 297 WP_186625170.1 DUF2845 domain-containing protein -
  HU754_RS25585 (HU754_025585) - 5803026..5803382 (-) 357 WP_077574577.1 BON domain-containing protein -
  HU754_RS25590 (HU754_025590) pilA 5803678..5804109 (-) 432 WP_093434881.1 pilin Machinery gene
  HU754_RS25595 (HU754_025595) pilB 5804336..5806036 (+) 1701 WP_093434878.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HU754_RS25600 (HU754_025600) pilC 5806039..5807256 (+) 1218 WP_093434875.1 type II secretion system F family protein Machinery gene
  HU754_RS25605 (HU754_025605) pilD 5807258..5808130 (+) 873 WP_093434872.1 A24 family peptidase Machinery gene
  HU754_RS25610 (HU754_025610) coaE 5808127..5808750 (+) 624 WP_093434870.1 dephospho-CoA kinase -
  HU754_RS25615 (HU754_025615) yacG 5808747..5808947 (+) 201 WP_186625120.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 143 a.a.        Molecular weight: 14337.50 Da        Isoelectric Point: 8.4936

>NTDB_id=579056 HU754_RS25590 WP_093434881.1 5803678..5804109(-) (pilA) [Pseudomonas zeae strain OE 48.2]
MKKQQGFTLIELLIVVAIIGILATIALPQYSKYQARSKVTAGLAEISALKVPFEDTINQGTDPTLTLVAGSATAATSNCT
LAASGTASTGAGTITCTLLNAPGPVLSKTITLTRSSGTGAGVWTCGTTVAPDYSPKGCTATGT

Nucleotide


Download         Length: 432 bp        

>NTDB_id=579056 HU754_RS25590 WP_093434881.1 5803678..5804109(-) (pilA) [Pseudomonas zeae strain OE 48.2]
ATGAAAAAACAACAAGGTTTTACTCTGATCGAGCTGCTGATCGTGGTGGCGATCATCGGGATTCTGGCGACCATCGCGTT
GCCGCAGTATTCCAAGTATCAGGCGCGGTCGAAGGTGACGGCGGGGCTGGCGGAAATCAGCGCGCTGAAGGTGCCGTTTG
AAGACACCATCAATCAAGGCACTGATCCGACTCTGACCCTAGTCGCGGGCAGTGCCACCGCTGCGACTTCCAACTGCACA
CTGGCCGCTTCCGGCACGGCATCCACCGGTGCCGGGACCATCACTTGCACATTGCTCAATGCTCCGGGTCCGGTGCTGAG
CAAAACCATCACCCTGACACGTTCCTCAGGGACCGGCGCAGGTGTGTGGACTTGTGGTACCACCGTTGCTCCGGACTATT
CGCCTAAAGGCTGCACTGCCACCGGTACATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae C6706

45.395

100

0.483
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

45.395

100

0.483
  pilA Vibrio cholerae strain A1552

45.395

100

0.483
  pilA Acinetobacter baumannii strain A118

47.887

99.301

0.476
  pilA/pilAI Pseudomonas stutzeri DSM 10701

44.928

96.503

0.434
  pilA Pseudomonas aeruginosa PAK

41.06

100

0.434
  comP Acinetobacter baylyi ADP1

39.61

100

0.427
  pilA/pilAII Pseudomonas stutzeri DSM 10701

43.478

96.503

0.42
  pilA Vibrio parahaemolyticus RIMD 2210633

40.278

100

0.406
  pilA2 Legionella pneumophila str. Paris

40.714

97.902

0.399
  pilA2 Legionella pneumophila strain ERS1305867

40.714

97.902

0.399
  pilA Ralstonia pseudosolanacearum GMI1000

34.783

100

0.392