Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   HU754_RS25600 Genome accession   NZ_CP077090
Coordinates   5806039..5807256 (+) Length   405 a.a.
NCBI ID   WP_093434875.1    Uniprot ID   -
Organism   Pseudomonas zeae strain OE 48.2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5801039..5812256
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HU754_RS25580 (HU754_025580) - 5802460..5802756 (+) 297 WP_186625170.1 DUF2845 domain-containing protein -
  HU754_RS25585 (HU754_025585) - 5803026..5803382 (-) 357 WP_077574577.1 BON domain-containing protein -
  HU754_RS25590 (HU754_025590) pilA 5803678..5804109 (-) 432 WP_093434881.1 pilin Machinery gene
  HU754_RS25595 (HU754_025595) pilB 5804336..5806036 (+) 1701 WP_093434878.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HU754_RS25600 (HU754_025600) pilC 5806039..5807256 (+) 1218 WP_093434875.1 type II secretion system F family protein Machinery gene
  HU754_RS25605 (HU754_025605) pilD 5807258..5808130 (+) 873 WP_093434872.1 A24 family peptidase Machinery gene
  HU754_RS25610 (HU754_025610) coaE 5808127..5808750 (+) 624 WP_093434870.1 dephospho-CoA kinase -
  HU754_RS25615 (HU754_025615) yacG 5808747..5808947 (+) 201 WP_186625120.1 DNA gyrase inhibitor YacG -
  HU754_RS25620 (HU754_025620) - 5808954..5809169 (-) 216 WP_007909901.1 hypothetical protein -
  HU754_RS25625 (HU754_025625) - 5809235..5809924 (-) 690 WP_186625111.1 energy-coupling factor ABC transporter permease -
  HU754_RS25630 (HU754_025630) - 5809921..5810388 (-) 468 WP_186625103.1 FAD/FMN-containing dehydrogenase -
  HU754_RS25635 (HU754_025635) - 5810519..5811145 (+) 627 WP_007909887.1 DUF1780 domain-containing protein -
  HU754_RS25640 (HU754_025640) - 5811142..5811669 (+) 528 WP_095048688.1 MOSC domain-containing protein -
  HU754_RS25645 (HU754_025645) - 5811721..5811894 (+) 174 WP_007909875.1 DUF3094 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 43968.65 Da        Isoelectric Point: 10.1093

>NTDB_id=579058 HU754_RS25600 WP_093434875.1 5806039..5807256(+) (pilC) [Pseudomonas zeae strain OE 48.2]
MAVKAAKISIYAWEGTDRKGSKVTGELSGQNPALIKAQLRKQGINPGKVRKKSASLLSFGKRIKARDIALFTRQMATMMK
AGVPLLQSFDIIGEGFDNPAMRKLVDDVKQEVAAGNSFAAALRKKPQYFDELYCNLVDAGEQSGALDTLLERVATYKEKS
ESLKAKIKKAMTYPTAVVLVAAVVTGILLVKVVPQFQSVFSGFGAELPAFTLMVISLSEFMQQWWWAILAALVAAIFGTR
HALKTSQALRDRKDTWLLKLPLVGTLMYKSAVARFARTLSTTFAAGVPLVEALDSVAGATGNVVFKRAVLRIRQDVSTGM
QLNFSMRSTGVFPSMAVQMTAIGEESGALDDMLDKVAGFYEEEVDNMVDNLTSLMEPFIMVVLGVIVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=579058 HU754_RS25600 WP_093434875.1 5806039..5807256(+) (pilC) [Pseudomonas zeae strain OE 48.2]
ATGGCGGTCAAGGCAGCAAAAATCAGTATTTATGCCTGGGAAGGCACGGACAGAAAAGGCAGCAAAGTCACCGGTGAACT
GAGCGGCCAGAACCCGGCGCTGATCAAGGCACAATTGCGTAAACAGGGGATCAATCCCGGCAAGGTGCGCAAGAAATCCG
CGTCATTGCTGAGCTTTGGCAAACGCATCAAGGCCCGGGACATTGCCCTGTTCACCCGGCAGATGGCGACCATGATGAAA
GCCGGCGTACCGCTGTTGCAGTCGTTCGACATTATTGGCGAAGGCTTTGATAACCCGGCCATGCGCAAACTGGTGGACGA
CGTGAAACAGGAGGTCGCGGCCGGTAACAGCTTCGCCGCAGCCCTGCGCAAGAAACCGCAGTATTTCGACGAGTTGTATT
GCAACCTGGTCGATGCCGGCGAGCAGTCCGGCGCCCTCGACACCTTGCTCGAACGCGTCGCGACCTATAAGGAAAAAAGC
GAAAGCCTCAAAGCCAAGATCAAGAAAGCCATGACCTATCCCACCGCCGTGGTGCTGGTCGCGGCTGTTGTCACCGGGAT
TCTGCTGGTCAAAGTAGTGCCACAATTTCAGTCAGTGTTTTCCGGTTTCGGTGCCGAACTGCCGGCGTTCACCCTGATGG
TGATCAGCCTGTCGGAATTCATGCAGCAATGGTGGTGGGCGATTCTCGCTGCACTGGTCGCAGCGATTTTTGGCACCCGC
CACGCGCTTAAAACATCCCAAGCCTTGCGCGATCGCAAAGACACCTGGCTGCTGAAACTGCCACTGGTGGGCACACTGAT
GTACAAGTCCGCCGTGGCGCGATTCGCTCGAACCTTGTCCACCACGTTTGCCGCCGGTGTGCCGTTGGTCGAAGCGCTTG
ACTCGGTGGCCGGCGCCACCGGCAACGTGGTCTTCAAGCGAGCCGTGCTGCGCATTCGCCAGGACGTGTCCACCGGTATG
CAACTGAATTTCTCGATGCGCAGCACCGGGGTCTTTCCCAGCATGGCCGTGCAAATGACCGCAATTGGCGAAGAGTCCGG
CGCCCTGGATGACATGCTCGACAAGGTTGCGGGCTTTTACGAGGAAGAAGTGGATAACATGGTCGACAACCTCACCAGCC
TCATGGAGCCGTTCATCATGGTCGTGCTGGGGGTAATCGTCGGCGGGCTGGTGGTGGCCATGTACCTGCCGATCTTCCAA
CTCGGCTCAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

75.309

100

0.753
  pilC Acinetobacter baumannii D1279779

59.559

100

0.6
  pilC Acinetobacter baylyi ADP1

58.824

100

0.593
  pilC Legionella pneumophila strain ERS1305867

53.535

97.778

0.523
  pilC Vibrio cholerae strain A1552

44.697

97.778

0.437
  pilG Neisseria gonorrhoeae MS11

43.176

99.506

0.43
  pilG Neisseria meningitidis 44/76-A

42.68

99.506

0.425
  pilC Vibrio campbellii strain DS40M4

41.626

100

0.417
  pilC Thermus thermophilus HB27

38.653

99.012

0.383