Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HU754_RS25595 Genome accession   NZ_CP077090
Coordinates   5804336..5806036 (+) Length   566 a.a.
NCBI ID   WP_093434878.1    Uniprot ID   -
Organism   Pseudomonas zeae strain OE 48.2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5799336..5811036
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HU754_RS25575 (HU754_025575) - 5799512..5802460 (+) 2949 WP_186625122.1 DUF748 domain-containing protein -
  HU754_RS25580 (HU754_025580) - 5802460..5802756 (+) 297 WP_186625170.1 DUF2845 domain-containing protein -
  HU754_RS25585 (HU754_025585) - 5803026..5803382 (-) 357 WP_077574577.1 BON domain-containing protein -
  HU754_RS25590 (HU754_025590) pilA 5803678..5804109 (-) 432 WP_093434881.1 pilin Machinery gene
  HU754_RS25595 (HU754_025595) pilB 5804336..5806036 (+) 1701 WP_093434878.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HU754_RS25600 (HU754_025600) pilC 5806039..5807256 (+) 1218 WP_093434875.1 type II secretion system F family protein Machinery gene
  HU754_RS25605 (HU754_025605) pilD 5807258..5808130 (+) 873 WP_093434872.1 A24 family peptidase Machinery gene
  HU754_RS25610 (HU754_025610) coaE 5808127..5808750 (+) 624 WP_093434870.1 dephospho-CoA kinase -
  HU754_RS25615 (HU754_025615) yacG 5808747..5808947 (+) 201 WP_186625120.1 DNA gyrase inhibitor YacG -
  HU754_RS25620 (HU754_025620) - 5808954..5809169 (-) 216 WP_007909901.1 hypothetical protein -
  HU754_RS25625 (HU754_025625) - 5809235..5809924 (-) 690 WP_186625111.1 energy-coupling factor ABC transporter permease -
  HU754_RS25630 (HU754_025630) - 5809921..5810388 (-) 468 WP_186625103.1 FAD/FMN-containing dehydrogenase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62691.69 Da        Isoelectric Point: 5.9184

>NTDB_id=579057 HU754_RS25595 WP_093434878.1 5804336..5806036(+) (pilB) [Pseudomonas zeae strain OE 48.2]
MNDIALSGLAKQLVQAELLTEKGAQQAWQQAQRNRLSLVSYLVQNKLVKSWQVAEIASEHFGMALLDLNCLDKETQPKGL
VSEKLVRQHHALPLWRRGNKLFVGISDPSNHQAINDIQFSTGLSTEAILVEDDKLTDAIEKFFDTHTSGLEDMADVDLDG
VDVESVDDSKQDAIGGLDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNYRVRVRTDGILREVAKPPIQLAGRIAARL
KVMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQIGIDALGYEPEQKDLYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPKQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCSHCKKAIEIPRETLLKEGFPE
ERIGHFTIYEPVGCDHCNGGYKGRVGIYEVVKNTPELQRLIMAEGNSLEIDLQMRRDGFDDLRTSGLHKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=579057 HU754_RS25595 WP_093434878.1 5804336..5806036(+) (pilB) [Pseudomonas zeae strain OE 48.2]
ATGAATGACATCGCTCTGAGCGGTCTGGCCAAGCAATTGGTCCAGGCCGAACTGCTCACGGAAAAAGGCGCCCAGCAAGC
CTGGCAACAGGCCCAGCGCAATCGCCTGTCGCTGGTCAGCTATCTGGTGCAGAACAAACTGGTCAAGAGCTGGCAGGTGG
CCGAGATTGCCAGTGAGCATTTCGGCATGGCCTTGCTTGATCTCAATTGCCTGGACAAGGAAACCCAGCCCAAAGGCCTG
GTCAGCGAAAAACTCGTGCGTCAGCATCACGCCCTGCCCTTGTGGCGGCGTGGCAACAAGCTGTTCGTGGGCATTTCCGA
CCCGAGCAATCATCAAGCGATCAACGACATTCAATTCAGCACCGGGCTGAGCACCGAAGCCATTCTGGTCGAGGACGACA
AACTTACCGATGCCATCGAAAAATTCTTCGACACCCACACCAGCGGTCTCGAGGACATGGCCGATGTCGATCTCGACGGG
GTCGATGTCGAATCCGTCGACGACAGCAAGCAGGACGCCATCGGCGGCCTCGACGCCGACGATGCGCCGGTGGTGCGCTT
CGTGCACAAGATGCTGCTCGACGCGATCAAAAGCGGTTCTTCCGACTTGCACTTCGAGCCTTACGAAAAAAACTACCGGG
TGCGGGTGCGCACCGACGGCATCCTGCGCGAGGTTGCCAAACCGCCGATTCAACTGGCCGGGCGCATCGCCGCACGCCTG
AAAGTCATGGCCAGCCTGGATATTTCGGAACGGCGCAAACCTCAGGACGGGCGGATCAAGATGCGCCTGTCGAAAAGCAA
GTCGATCGATTTCCGGGTCAACACGCTGCCGACCCTGTGGGGCGAAAAAGTGGTGATCCGGATTCTCGACCCGTCCAGCG
CGCAGATCGGTATCGACGCCCTCGGCTATGAGCCCGAGCAAAAAGATCTGTACATGGCCGCACTCAAGCAGCCACAGGGC
ATGATCCTGGTCACCGGGCCCACCGGTTCGGGTAAAACCGTTTCGCTGTACACCGGCCTGAATATTCTCAACACCGTCGA
CATCAACATCTCCACCGCCGAAGACCCGGTGGAAATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCCAAGCAAG
GGCTCGACTTTGCCCAGGCATTGCGCTCGTTTCTGCGTCAGGACCCGGACGTGATCATGGTCGGTGAAATCCGCGACCTG
GAAACCGCCGAGATCGCCATCAAAGCCGCGCAGACCGGTCACCTGGTGCTCTCGACCCTGCACACCAACAGCGCTGCGGA
AACCCTGACGCGCCTGCACAACATGGGCATTCCCGGTTTCAACATCGCCACCTCGGTCAGCCTGATCATCGCCCAGCGCC
TGGCGCGCAAACTGTGCAGCCACTGCAAGAAAGCCATCGAGATCCCGCGCGAAACCTTGCTCAAGGAAGGCTTCCCCGAG
GAACGCATCGGCCATTTCACGATCTACGAGCCGGTCGGTTGCGATCACTGCAACGGCGGTTACAAAGGACGCGTGGGGAT
TTATGAAGTGGTGAAAAACACACCGGAGCTGCAACGCTTGATCATGGCCGAAGGCAACTCGCTGGAAATCGACCTGCAGA
TGCGCCGCGACGGCTTCGACGACCTGCGCACCTCCGGGCTGCACAAGGCCATGCAAGGCATCACCAGCCTTGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.97

99.47

0.587
  pilB Acinetobacter baumannii D1279779

58.156

99.647

0.58
  pilB Legionella pneumophila strain ERS1305867

56.007

100

0.56
  pilB Vibrio cholerae strain A1552

51.327

99.823

0.512
  pilF Neisseria gonorrhoeae MS11

49.911

99.47

0.496
  pilB Vibrio parahaemolyticus RIMD 2210633

49.911

99.47

0.496
  pilB Vibrio campbellii strain DS40M4

48.264

100

0.491
  pilF Thermus thermophilus HB27

36.918

98.587

0.364