Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   KLP38_RS14030 Genome accession   NZ_CP076060
Coordinates   2933805..2934944 (-) Length   379 a.a.
NCBI ID   WP_215528496.1    Uniprot ID   -
Organism   Cupriavidus sp. EM10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2928805..2939944
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KLP38_RS14005 (KLP38_14005) glcF 2929827..2931104 (+) 1278 WP_215528493.1 glycolate oxidase subunit GlcF -
  KLP38_RS14010 (KLP38_14010) - 2931331..2931716 (+) 386 Protein_2803 CidA/LrgA family protein -
  KLP38_RS14015 (KLP38_14015) - 2931713..2932437 (+) 725 Protein_2804 LrgB family protein -
  KLP38_RS14020 (KLP38_14020) - 2932538..2933224 (+) 687 WP_215528494.1 YafY family protein -
  KLP38_RS14025 (KLP38_14025) - 2933255..2933749 (-) 495 WP_215528495.1 glutathione peroxidase -
  KLP38_RS14030 (KLP38_14030) pilU 2933805..2934944 (-) 1140 WP_215528496.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KLP38_RS14035 (KLP38_14035) - 2934955..2935996 (-) 1042 Protein_2808 type IV pilus twitching motility protein PilT -
  KLP38_RS14040 (KLP38_14040) - 2936108..2936797 (+) 690 WP_215528497.1 YggS family pyridoxal phosphate-dependent enzyme -
  KLP38_RS14045 (KLP38_14045) proC 2936893..2937711 (+) 819 WP_215528498.1 pyrroline-5-carboxylate reductase -
  KLP38_RS14050 (KLP38_14050) - 2937903..2938853 (+) 951 WP_215528499.1 transposase -
  KLP38_RS14055 (KLP38_14055) ubiA 2938903..2939814 (-) 912 WP_370649065.1 4-hydroxybenzoate octaprenyltransferase -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42475.69 Da        Isoelectric Point: 6.4437

>NTDB_id=571750 KLP38_RS14030 WP_215528496.1 2933805..2934944(-) (pilU) [Cupriavidus sp. EM10]
MLDRESASKYINDLLELMVNNRGSDLFITSEFPPAIKVDGKITPVSQQPLNPTQALGLVRSIMNERQVKEFDDTRECNFA
ITAPNAGRFRVSAFIQQGRAGMVVRTINTRIPSVEDLDLPQSLHDIVMSKRGLVIVTGATGSGKSTSLAAMLDHRNAHSY
GHIITIEDPIEYVHAHQNCIVTQREVGIDTESWHIALKNTLRQAPDVILIGEIRDRETMEYAMQYAETGHLCLATLHANN
ANQAIDRIVNFFPEEKRQQLLIDLSLNLKAMISQRLLPRAGRKGRVPAVEIMIGTPLVADLIFKGEIHELKEVIKKSREQ
GMISFDQALFELYEAEKITYEDALKNADSLNDLRLMIKLHSARHRDADLGAGTEHLNVI

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=571750 KLP38_RS14030 WP_215528496.1 2933805..2934944(-) (pilU) [Cupriavidus sp. EM10]
ATGCTCGACCGTGAATCCGCATCGAAGTACATCAACGACCTGCTCGAGCTGATGGTGAACAACCGTGGCTCGGACCTGTT
CATTACCTCCGAATTCCCGCCCGCCATCAAGGTCGACGGCAAGATCACGCCGGTGTCGCAGCAGCCGCTGAACCCGACGC
AGGCGCTGGGCCTGGTGCGTTCGATCATGAACGAGCGCCAGGTGAAGGAATTCGACGACACGCGCGAATGCAACTTCGCG
ATCACGGCGCCCAACGCGGGCCGCTTCCGGGTGTCGGCGTTCATCCAGCAGGGCCGCGCCGGCATGGTGGTGCGGACGAT
CAACACGCGCATCCCGTCGGTGGAAGACCTGGACCTGCCGCAGTCGCTGCATGACATCGTGATGTCCAAGCGCGGGCTGG
TGATCGTGACGGGCGCCACGGGCTCGGGCAAGTCGACGTCGCTGGCGGCGATGCTGGACCACCGCAACGCGCATTCGTAC
GGCCACATCATCACGATCGAGGATCCGATCGAATACGTGCACGCGCACCAGAACTGCATCGTCACGCAGCGCGAGGTGGG
CATCGATACCGAGTCGTGGCACATCGCGCTGAAGAACACGCTGCGCCAGGCGCCCGACGTGATCCTGATCGGCGAAATCC
GCGACCGCGAGACGATGGAATACGCCATGCAGTACGCCGAAACGGGCCACCTGTGCCTGGCCACGCTGCACGCGAACAAC
GCCAACCAGGCCATCGACCGGATCGTCAATTTCTTCCCCGAGGAAAAGCGCCAGCAGCTGCTGATCGACCTGTCGCTGAA
CCTGAAGGCGATGATCTCGCAGCGCCTGCTGCCGCGCGCCGGCCGCAAGGGCCGCGTACCGGCCGTGGAAATCATGATCG
GCACGCCGCTGGTGGCCGACCTGATCTTCAAGGGCGAGATCCACGAGCTCAAGGAAGTCATCAAGAAATCGCGCGAACAG
GGCATGATCTCGTTCGACCAGGCGCTGTTCGAGCTGTACGAGGCCGAGAAGATCACCTACGAGGACGCGCTCAAGAACGC
CGACTCGCTGAACGACCTGCGGCTGATGATCAAGCTCCATAGCGCGCGGCATCGCGACGCCGATCTTGGCGCCGGCACCG
AGCACCTGAACGTGATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(37-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.218

91.821

0.58
  pilU Acinetobacter baylyi ADP1

59.143

92.348

0.546
  pilU Vibrio cholerae strain A1552

53.994

95.778

0.517
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.807

88.918

0.398
  pilT Legionella pneumophila strain Lp02

43.844

87.863

0.385
  pilT Legionella pneumophila strain ERS1305867

43.844

87.863

0.385
  pilT Pseudomonas stutzeri DSM 10701

43.323

88.918

0.385
  pilT Pseudomonas aeruginosa PAK

42.73

88.918

0.38
  pilT Acinetobacter baylyi ADP1

44.41

84.96

0.377
  pilT Acinetobacter nosocomialis M2

44.514

84.169

0.375
  pilT Acinetobacter baumannii D1279779

44.514

84.169

0.375
  pilT Acinetobacter baumannii strain A118

44.514

84.169

0.375
  pilT Neisseria meningitidis 8013

41.124

89.182

0.367
  pilT Vibrio cholerae strain A1552

43.218

83.641

0.361
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.218

83.641

0.361