Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KKI46_RS11775 Genome accession   NZ_CP075897
Coordinates   2217948..2218994 (-) Length   348 a.a.
NCBI ID   WP_029342266.1    Uniprot ID   -
Organism   Exiguobacterium acetylicum strain SI17     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2212948..2223994
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KKI46_RS11755 (KKI46_11755) - 2213493..2215229 (-) 1737 WP_069940872.1 fimbrial assembly protein -
  KKI46_RS11760 (KKI46_11760) - 2215248..2216003 (-) 756 WP_029342263.1 A24 family peptidase -
  KKI46_RS11765 (KKI46_11765) - 2216064..2216588 (-) 525 WP_029342264.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  KKI46_RS11770 (KKI46_11770) - 2216726..2217943 (-) 1218 WP_029342265.1 type II secretion system F family protein -
  KKI46_RS11775 (KKI46_11775) pilT 2217948..2218994 (-) 1047 WP_029342266.1 type IV pilus twitching motility protein PilT Machinery gene
  KKI46_RS11780 (KKI46_11780) - 2218991..2220655 (-) 1665 WP_047393614.1 ATPase, T2SS/T4P/T4SS family -
  KKI46_RS11785 (KKI46_11785) - 2220664..2220978 (-) 315 WP_029342268.1 hypothetical protein -
  KKI46_RS11790 (KKI46_11790) - 2220994..2222355 (-) 1362 WP_244989002.1 G5 domain-containing protein -
  KKI46_RS11795 (KKI46_11795) - 2222545..2223072 (+) 528 WP_214727454.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  KKI46_RS11800 (KKI46_11800) - 2223035..2223655 (+) 621 WP_029342271.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 38152.84 Da        Isoelectric Point: 7.1317

>NTDB_id=570796 KKI46_RS11775 WP_029342266.1 2217948..2218994(-) (pilT) [Exiguobacterium acetylicum strain SI17]
MNSTIERAQIEDILRSSKERGASDVHLTAGSPPVFRINGTLTPFGTEKMKPIIIDGFVQSLITEEMYQQLKQDRDIDFSF
GVTGVARYRVNCFYQRGALSMAFRTIPTEVPTLEQLALPPVLKRFLTKPHGLILVTGPTGSGKSTTLAAMINEINQTMYK
RIITLEDPIEYLHSHHKSLIDQREIGIDAPRFATGLRSALRQDPDVILLGEMRDLETIATAVTAAETGHLVFATVHTSTA
ASTVSRIIDVFPSEQQDQIRAQLANVLAGVVSQRLMPRSDGTGRVAALEIMVDTPAVSNLIRTGKEYQIQSVLQTGKQYG
MQTMQMALQDLVSRGLIPASAASPYISG

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=570796 KKI46_RS11775 WP_029342266.1 2217948..2218994(-) (pilT) [Exiguobacterium acetylicum strain SI17]
ATGAACAGTACGATCGAACGGGCACAAATCGAAGATATTTTACGTTCGTCGAAAGAGCGAGGCGCATCCGATGTTCACTT
GACGGCGGGATCACCGCCCGTCTTTCGGATCAACGGAACGTTGACACCGTTTGGAACAGAAAAGATGAAACCGATCATCA
TTGATGGATTTGTTCAGTCGTTGATCACGGAAGAAATGTATCAACAGCTGAAGCAGGACCGGGATATCGATTTTTCGTTC
GGTGTGACCGGTGTCGCCCGGTACCGGGTCAACTGTTTTTACCAACGGGGCGCTTTGTCGATGGCGTTTCGAACGATTCC
TACCGAGGTGCCAACGCTTGAGCAATTAGCGTTGCCGCCTGTCTTAAAGCGATTCTTAACGAAACCACACGGCTTGATTC
TCGTGACGGGTCCGACGGGTTCCGGAAAATCGACGACGCTTGCAGCGATGATCAATGAAATTAATCAGACGATGTATAAA
CGGATCATTACGCTGGAGGATCCGATTGAGTACTTACATAGTCATCATAAAAGTCTTATTGATCAGCGGGAAATCGGGAT
TGATGCGCCGCGCTTTGCGACTGGATTACGGTCAGCGCTCCGTCAAGATCCGGATGTCATTTTGCTTGGAGAGATGCGAG
ACCTTGAAACGATCGCGACCGCCGTGACAGCAGCAGAAACTGGTCACCTCGTCTTTGCAACGGTCCATACGTCGACGGCT
GCCTCGACAGTCAGCCGGATCATCGATGTTTTCCCGTCAGAGCAACAAGATCAGATTCGGGCTCAACTCGCAAACGTTCT
TGCGGGTGTCGTCTCGCAACGCCTGATGCCGCGTTCGGATGGAACTGGACGCGTCGCAGCCCTTGAAATCATGGTCGATA
CACCAGCTGTCTCGAACTTGATTCGGACCGGTAAGGAATATCAGATTCAATCCGTCCTGCAGACGGGTAAACAATACGGC
ATGCAGACGATGCAGATGGCATTGCAGGATCTCGTCAGTCGGGGATTGATTCCGGCTTCTGCTGCATCACCGTACATATC
GGGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.524

94.253

0.486
  pilT Neisseria gonorrhoeae MS11

50

95.977

0.48
  pilT Neisseria meningitidis 8013

50

95.977

0.48
  pilT Acinetobacter nosocomialis M2

48.949

95.69

0.468
  pilT Pseudomonas aeruginosa PAK

48.949

95.69

0.468
  pilT Acinetobacter baylyi ADP1

47.941

97.701

0.468
  pilT Acinetobacter baumannii D1279779

48.649

95.69

0.466
  pilT Acinetobacter baumannii strain A118

48.649

95.69

0.466
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.091

94.828

0.466
  pilT Vibrio cholerae strain A1552

49.091

94.828

0.466
  pilT Pseudomonas stutzeri DSM 10701

47.748

95.69

0.457
  pilT Legionella pneumophila strain Lp02

46.246

95.69

0.443
  pilT Legionella pneumophila strain ERS1305867

46.246

95.69

0.443
  pilU Acinetobacter baylyi ADP1

40.361

95.402

0.385
  pilU Pseudomonas stutzeri DSM 10701

40.426

94.54

0.382