Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KHO27_RS20420 Genome accession   NZ_CP075595
Coordinates   4486125..4487117 (-) Length   330 a.a.
NCBI ID   WP_110697359.1    Uniprot ID   -
Organism   Pseudomonas sp. RC3H12     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4481125..4492117
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KHO27_RS20400 (KHO27_20400) argS 4482368..4484104 (+) 1737 WP_110697363.1 arginine--tRNA ligase -
  KHO27_RS20405 (KHO27_20405) - 4484106..4484807 (+) 702 WP_102681779.1 SPOR domain-containing protein -
  KHO27_RS20410 (KHO27_20410) - 4484845..4485270 (-) 426 WP_102681780.1 TM2 domain-containing protein -
  KHO27_RS20415 (KHO27_20415) - 4485428..4486075 (+) 648 WP_110697361.1 NlpC/P60 family protein -
  KHO27_RS20420 (KHO27_20420) pilT 4486125..4487117 (-) 993 WP_110697359.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KHO27_RS20425 (KHO27_20425) - 4487183..4487869 (+) 687 WP_110697357.1 YggS family pyridoxal phosphate-dependent enzyme -
  KHO27_RS20430 (KHO27_20430) proC 4487909..4488727 (+) 819 WP_110697355.1 pyrroline-5-carboxylate reductase -
  KHO27_RS20435 (KHO27_20435) - 4488738..4489328 (+) 591 WP_023630880.1 YggT family protein -
  KHO27_RS20440 (KHO27_20440) - 4489417..4490556 (+) 1140 WP_214500696.1 homoserine O-acetyltransferase -
  KHO27_RS20445 (KHO27_20445) metW 4490564..4491184 (+) 621 WP_110697353.1 methionine biosynthesis protein MetW -
  KHO27_RS20450 (KHO27_20450) - 4491207..4491641 (+) 435 WP_110697351.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 330 a.a.        Molecular weight: 35619.33 Da        Isoelectric Point: 6.6488

>NTDB_id=569808 KHO27_RS20420 WP_110697359.1 4486125..4487117(-) (pilT) [Pseudomonas sp. RC3H12]
MDVTDLLGRAVAAGASDLHLAVGEPPLLRLDGELQRLAVPVLTCAALEQGMAGLLDDRQRQQWLSGDELDLALSLPGVGR
LRLNLFRQRNGLGASVRLIPARVPSLDELELNDVYQVVAQCRDGLVLVGGPTGSGKSSTLAALLDQINRERALQVITLED
PIEFIHSGQACLVNQREIGVHSRDFAQGLRSALRQDPDVIMVGELRDLESIRLALRAAETGHLVLATVHTRSAVSSIDRL
VEVFAADEKPLVRMMLAESLRLVVAQVLVRRVGGGRVAAREVLMATPAVRNLIREGRMAQLQSVMQAGAALGMRTMEGAM
QALRQKGLVD

Nucleotide


Download         Length: 993 bp        

>NTDB_id=569808 KHO27_RS20420 WP_110697359.1 4486125..4487117(-) (pilT) [Pseudomonas sp. RC3H12]
ATGGATGTGACCGACCTGCTGGGCCGGGCCGTGGCCGCGGGCGCCAGCGATCTGCACCTGGCCGTGGGCGAGCCGCCGTT
GCTGCGCCTGGATGGCGAGCTGCAGCGCCTGGCGGTGCCGGTGCTGACCTGCGCAGCGCTGGAGCAGGGAATGGCCGGCC
TGCTCGATGACCGGCAGCGGCAGCAATGGCTAAGCGGTGATGAACTGGACCTGGCGCTGAGCTTGCCCGGCGTGGGGCGG
CTGCGCTTGAACCTGTTCCGCCAGCGCAATGGGCTGGGCGCAAGCGTGCGGCTGATCCCGGCGCGGGTTCCCAGCCTCGA
TGAACTTGAGCTGAACGATGTGTATCAAGTCGTTGCGCAATGCCGGGATGGCCTGGTGCTGGTGGGCGGGCCGACGGGTA
GCGGCAAGTCCAGCACTCTGGCGGCGCTGCTCGATCAGATCAATCGCGAACGCGCGTTACAGGTCATCACCCTTGAAGAC
CCTATCGAATTTATCCACAGCGGCCAGGCTTGCCTGGTCAACCAGCGGGAGATCGGCGTGCACAGCCGTGACTTCGCCCA
AGGGCTGCGCAGTGCCCTGCGCCAGGACCCGGATGTGATCATGGTGGGTGAGTTGCGTGATCTGGAGAGTATCCGTCTGG
CGTTGCGCGCGGCAGAAACCGGGCACCTGGTGCTGGCAACGGTGCACACCCGCTCGGCGGTGAGCAGCATCGACCGGCTG
GTGGAGGTGTTCGCGGCCGACGAGAAGCCGCTGGTGCGCATGATGCTGGCCGAATCACTGCGCCTGGTGGTGGCTCAGGT
GCTGGTCAGGCGGGTGGGCGGCGGCAGGGTCGCGGCACGGGAGGTGCTGATGGCGACGCCGGCGGTGCGCAACCTGATTC
GCGAGGGGCGCATGGCACAGCTGCAGTCGGTGATGCAGGCGGGTGCGGCGCTGGGAATGCGGACGATGGAAGGGGCGATG
CAGGCGCTCAGGCAGAAAGGGCTGGTTGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.606

100

0.506
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.606

100

0.506
  pilT Pseudomonas aeruginosa PAK

50.152

99.697

0.5
  pilT Pseudomonas stutzeri DSM 10701

49.544

99.697

0.494
  pilT Acinetobacter baylyi ADP1

49.091

100

0.491
  pilT Acinetobacter baumannii strain A118

48.632

99.697

0.485
  pilT Acinetobacter nosocomialis M2

48.632

99.697

0.485
  pilT Acinetobacter baumannii D1279779

48.632

99.697

0.485
  pilT Neisseria meningitidis 8013

47.576

100

0.476
  pilT Neisseria gonorrhoeae MS11

47.273

100

0.473
  pilT Legionella pneumophila strain ERS1305867

47.416

99.697

0.473
  pilT Legionella pneumophila strain Lp02

47.416

99.697

0.473
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.455

100

0.455
  pilU Pseudomonas stutzeri DSM 10701

38.415

99.394

0.382
  pilU Vibrio cholerae strain A1552

36.728

98.182

0.361