Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   A7S10_RS04325 Genome accession   NZ_CP075135
Coordinates   897177..898157 (+) Length   326 a.a.
NCBI ID   WP_001646196.1    Uniprot ID   -
Organism   Salmonella enterica strain CFSAN044888     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 892177..903157
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A7S10_RS04295 (A7S10_004290) - 892754..893761 (+) 1008 WP_000252186.1 DUF1202 family protein -
  A7S10_RS04300 (A7S10_004295) hemW 893853..894989 (-) 1137 WP_023207649.1 radical SAM family heme chaperone HemW -
  A7S10_RS04305 (A7S10_004300) - 894982..895575 (-) 594 WP_070794159.1 XTP/dITP diphosphatase -
  A7S10_RS04310 (A7S10_004305) yggU 895583..895873 (-) 291 WP_001277205.1 DUF167 family protein YggU -
  A7S10_RS04315 (A7S10_004310) - 895870..896436 (-) 567 WP_001094848.1 YggT family protein -
  A7S10_RS04320 (A7S10_004315) - 896455..897159 (-) 705 WP_000997790.1 YggS family pyridoxal phosphate-dependent enzyme -
  A7S10_RS04325 (A7S10_004320) pilT 897177..898157 (+) 981 WP_001646196.1 type IV pilus twitching motility protein PilT Machinery gene
  A7S10_RS04330 (A7S10_004325) - 898285..898986 (+) 702 WP_023207647.1 IclR family transcriptional regulator -
  A7S10_RS04335 (A7S10_004330) ruvX 899018..899434 (-) 417 WP_000017096.1 Holliday junction resolvase RuvX -
  A7S10_RS04340 (A7S10_004335) - 899434..899997 (-) 564 WP_001053167.1 YqgE/AlgH family protein -
  A7S10_RS04345 (A7S10_004340) gshB 900213..901160 (-) 948 WP_000593251.1 glutathione synthase -
  A7S10_RS04350 (A7S10_004345) rsmE 901180..901911 (-) 732 WP_001222488.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  A7S10_RS04355 (A7S10_004350) endA 901988..902695 (-) 708 WP_000286116.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36182.42 Da        Isoelectric Point: 7.5116

>NTDB_id=568040 A7S10_RS04325 WP_001646196.1 897177..898157(+) (pilT) [Salmonella enterica strain CFSAN044888]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMEIAPFTAPDVDRLLLDWLNDAQQYQWRTHGQLDFAVSLSGTRRLRV
SAFTHQQGTSLALRLLPERCTDLAEIQTPPIVPALLASENGLILVTGATGCGKSTTLAAMVGYLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=568040 A7S10_RS04325 WP_001646196.1 897177..898157(+) (pilT) [Salmonella enterica strain CFSAN044888]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGATCGCGCCTTTTACCGCGCCAGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG
ACGCGCAACAATATCAGTGGCGAACGCACGGCCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTGCGCGTC
AGTGCGTTCACACATCAACAGGGAACGTCTCTGGCGCTACGGCTATTGCCCGAGCGTTGTACTGATCTGGCGGAAATCCA
GACGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCTACTGGCTGCGGGAAGT
CCACCACGCTGGCGGCGATGGTGGGATATCTTAATCAACATGCCGATAAACACATCCTGACGCTGGAAGATCCTATTGAG
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCGGCGTTGCGTGAAGATCCCGATGTCATTTTGCTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGTGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGGTACGCAGTCAACTGGCGGGTAGTCTGCGCGCTGTACTATCGCAAAAACTGGAGGT
CGACAGGCAGGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGCGCAGGGACGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(64-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.93

100

0.491
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.93

100

0.491
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Acinetobacter baumannii strain A118

45.566

100

0.457
  pilT Acinetobacter baumannii D1279779

45.566

100

0.457
  pilT Acinetobacter baylyi ADP1

45.566

100

0.457
  pilT Pseudomonas aeruginosa PAK

45.566

100

0.457
  pilT Pseudomonas stutzeri DSM 10701

45.26

100

0.454
  pilT Acinetobacter nosocomialis M2

45.26

100

0.454
  pilT Legionella pneumophila strain Lp02

45.283

97.546

0.442
  pilT Legionella pneumophila strain ERS1305867

45.283

97.546

0.442
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

38.871

97.853

0.38
  pilU Pseudomonas stutzeri DSM 10701

36.637

100

0.374