Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   B649_RS05060 Genome accession   NC_020505
Coordinates   978799..979869 (-) Length   356 a.a.
NCBI ID   WP_015653437.1    Uniprot ID   -
Organism   Candidatus Sulfuricurvum sp. RIFRC-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 973799..984869
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B649_RS05035 (B649_04970) - 974648..975130 (-) 483 WP_015653432.1 Fur family transcriptional regulator -
  B649_RS05040 (B649_04975) - 975134..975718 (-) 585 WP_015653433.1 CvpA family protein -
  B649_RS05045 (B649_04980) - 975718..976644 (-) 927 WP_015653434.1 GGDEF domain-containing protein -
  B649_RS05050 (B649_04985) - 976856..978202 (+) 1347 WP_015653435.1 class II 3-deoxy-7-phosphoheptulonate synthase -
  B649_RS05055 (B649_04990) - 978289..978693 (-) 405 WP_015653436.1 Fur family transcriptional regulator -
  B649_RS05060 (B649_04995) pilT 978799..979869 (-) 1071 WP_015653437.1 type IV pilus twitching motility protein PilT Machinery gene
  B649_RS05065 (B649_05000) - 979866..980210 (-) 345 WP_015653438.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  B649_RS05070 (B649_05005) gatC 980214..980504 (-) 291 WP_015653439.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC -
  B649_RS05075 (B649_05010) - 980627..981223 (+) 597 WP_015653440.1 hypothetical protein -
  B649_RS05080 (B649_05015) - 981223..982221 (+) 999 WP_015653441.1 PQQ-binding-like beta-propeller repeat protein -
  B649_RS05085 (B649_05020) - 982221..983042 (+) 822 WP_015653442.1 tyrosine-type recombinase/integrase -
  B649_RS05090 (B649_05025) - 983122..983997 (+) 876 WP_015653443.1 ORF6N domain-containing protein -
  B649_RS05095 (B649_05030) - 984073..984414 (+) 342 WP_015653444.1 cupin domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 39614.69 Da        Isoelectric Point: 6.6200

>NTDB_id=56515 B649_RS05060 WP_015653437.1 978799..979869(-) (pilT) [Candidatus Sulfuricurvum sp. RIFRC-1]
MNAALDIYKLLKSVVAYKASDLHLVSRSEPQIRIDGRLVALNLPVLDGRDIEEMGYSLLTEKQKKAFEEANELDFSIVIP
DVGRFRANYYKTMDDIACAFRIIPVTIPSLDDLNAKKIFKELIKREKGLILVTGPTGSGKSTTLAAMLNEINLNEARHII
TVEDPVEFVHTNKKSLFSHRNIGTDTKSFSAALKYALREDPDVILIGEMRDKETITAALTAAETGHLVFGTLHTNSAPQT
INRIIDVFSGDEQPQVRAQLSTSLIAVISQALLPKIGGGRIAAQELMITNPAISNLIREDKVHQLYSQMQLNQTGTSMTT
QTQEIVEFLRKKLITKEIALQYSNKPDELLRVIESL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=56515 B649_RS05060 WP_015653437.1 978799..979869(-) (pilT) [Candidatus Sulfuricurvum sp. RIFRC-1]
ATGAATGCAGCATTGGATATTTATAAACTCCTCAAGAGTGTCGTTGCGTATAAAGCATCAGATCTTCACCTCGTTAGCCG
TTCTGAACCCCAAATTCGTATCGATGGGCGGCTCGTTGCCCTTAATCTACCCGTTTTAGATGGCCGTGATATTGAAGAGA
TGGGCTATTCCCTCCTCACAGAAAAGCAAAAAAAAGCGTTTGAAGAGGCTAATGAGCTTGACTTTTCAATCGTTATCCCT
GATGTCGGCCGCTTTCGTGCCAACTACTACAAAACGATGGATGATATCGCATGCGCCTTTCGTATTATCCCTGTTACTAT
CCCCTCACTCGATGATTTGAACGCAAAAAAAATATTTAAAGAGCTTATTAAACGGGAAAAAGGGCTTATCCTCGTAACGG
GACCAACCGGAAGCGGTAAATCAACCACATTGGCGGCAATGCTCAATGAGATCAATCTCAATGAAGCCCGTCATATTATT
ACCGTTGAAGATCCCGTTGAGTTTGTCCATACCAATAAAAAATCGCTGTTCTCTCACCGTAATATCGGTACCGATACCAA
AAGCTTCTCGGCCGCACTCAAATATGCCCTTCGTGAAGACCCTGACGTTATCCTGATCGGGGAGATGCGGGATAAAGAGA
CAATCACTGCCGCACTAACCGCAGCAGAAACCGGTCACCTGGTTTTTGGAACACTCCATACCAACTCCGCACCTCAAACC
ATCAACCGTATTATCGACGTTTTCAGCGGAGACGAGCAGCCGCAGGTTCGAGCACAGCTCTCAACCTCATTGATCGCCGT
TATCTCTCAAGCGCTTCTCCCAAAAATCGGAGGCGGTCGTATTGCGGCGCAAGAACTGATGATTACCAACCCTGCAATTT
CGAATCTTATTCGTGAGGACAAAGTACATCAACTCTATTCACAAATGCAGCTCAACCAAACCGGTACAAGCATGACAACA
CAAACACAAGAGATTGTCGAATTTTTGCGTAAAAAGCTGATCACTAAAGAGATCGCGTTGCAATACTCCAATAAGCCCGA
TGAGTTACTGAGAGTCATCGAATCCCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii strain A118

51.437

97.753

0.503
  pilT Acinetobacter nosocomialis M2

51.437

97.753

0.503
  pilT Acinetobacter baumannii D1279779

51.437

97.753

0.503
  pilT Pseudomonas stutzeri DSM 10701

51.009

97.472

0.497
  pilT Acinetobacter baylyi ADP1

51.304

96.91

0.497
  pilT Legionella pneumophila strain Lp02

52.537

94.101

0.494
  pilT Legionella pneumophila strain ERS1305867

52.537

94.101

0.494
  pilT Pseudomonas aeruginosa PAK

50.432

97.472

0.492
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

53.438

89.888

0.48
  pilT Vibrio cholerae strain A1552

53.438

89.888

0.48
  pilT Neisseria gonorrhoeae MS11

48.844

97.191

0.475
  pilT Neisseria meningitidis 8013

48.555

97.191

0.472
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.507

99.719

0.444
  pilU Acinetobacter baylyi ADP1

39.655

97.753

0.388
  pilU Pseudomonas stutzeri DSM 10701

37.037

98.596

0.365

Multiple sequence alignment