Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   KHA79_RS04550 Genome accession   NZ_CP074364
Coordinates   1062543..1062977 (+) Length   144 a.a.
NCBI ID   WP_039005786.1    Uniprot ID   -
Organism   Xanthomonas translucens pv. cerealis strain CFBP 2541     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1057543..1067977
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KHA79_RS04530 (KHA79_04510) - 1057820..1059160 (+) 1341 WP_039005790.1 sensor histidine kinase -
  KHA79_RS04535 (KHA79_04515) coaE 1059398..1060018 (-) 621 WP_039005789.1 dephospho-CoA kinase -
  KHA79_RS04540 (KHA79_04520) - 1060032..1060895 (-) 864 WP_039005788.1 prepilin peptidase -
  KHA79_RS04545 (KHA79_04525) pilC 1060902..1062161 (-) 1260 WP_039005787.1 type II secretion system F family protein Machinery gene
  KHA79_RS04550 (KHA79_04530) pilA 1062543..1062977 (+) 435 WP_039005786.1 pilin Machinery gene
  KHA79_RS04555 (KHA79_04535) - 1063118..1063537 (-) 420 WP_200886351.1 hypothetical protein -
  KHA79_RS04560 (KHA79_04540) pilB 1063764..1065500 (+) 1737 WP_039005784.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KHA79_RS04565 (KHA79_04545) - 1065675..1066127 (-) 453 WP_230812364.1 Fic/DOC family protein -
  KHA79_RS04570 (KHA79_04550) - 1066124..1066306 (-) 183 WP_003477093.1 YhfG family protein -
  KHA79_RS04575 (KHA79_04555) pilR 1066495..1067877 (-) 1383 WP_039005783.1 sigma-54-dependent transcriptional regulator Regulator

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 14869.00 Da        Isoelectric Point: 8.1020

>NTDB_id=564280 KHA79_RS04550 WP_039005786.1 1062543..1062977(+) (pilA) [Xanthomonas translucens pv. cerealis strain CFBP 2541]
MKKQQGFTLIELMIVVAIIAILAAIALPAYQNYVAKSQVTTALADITGAKTGIEEALNRGVAPSLDTTAAGYVGVQSTSP
RCTTIALATFSAQSAEGNITCTIGNSSPKVNTKKVILTRDANGSWACTSDADPKYKPQGCGDAT

Nucleotide


Download         Length: 435 bp        

>NTDB_id=564280 KHA79_RS04550 WP_039005786.1 1062543..1062977(+) (pilA) [Xanthomonas translucens pv. cerealis strain CFBP 2541]
ATGAAGAAGCAGCAGGGCTTTACCCTGATCGAACTGATGATCGTCGTCGCGATCATCGCCATCCTGGCCGCCATCGCGCT
GCCGGCCTATCAGAACTATGTTGCCAAGTCGCAGGTAACGACCGCGCTTGCCGATATCACTGGCGCTAAGACCGGTATCG
AAGAAGCACTTAATCGAGGCGTTGCCCCTAGCTTGGATACCACTGCGGCTGGCTATGTGGGCGTCCAGTCTACGAGCCCC
CGTTGCACCACTATCGCGCTGGCTACTTTTTCTGCGCAAAGCGCAGAAGGCAACATCACGTGCACAATCGGCAATAGCAG
CCCGAAGGTTAATACCAAGAAGGTAATTTTGACCCGTGATGCTAACGGTTCTTGGGCCTGCACAAGCGACGCGGACCCTA
AATACAAGCCGCAGGGCTGTGGCGATGCTACATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

48.649

100

0.5
  pilA/pilAI Pseudomonas stutzeri DSM 10701

50.725

95.833

0.486
  pilA/pilAII Pseudomonas stutzeri DSM 10701

49.635

95.139

0.472
  pilA Acinetobacter baumannii strain A118

48.571

97.222

0.472
  comP Acinetobacter baylyi ADP1

43.919

100

0.451
  pilA Vibrio cholerae C6706

42.254

98.611

0.417
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

42.254

98.611

0.417
  pilA Vibrio cholerae strain A1552

42.254

98.611

0.417
  pilA2 Legionella pneumophila str. Paris

41.549

98.611

0.41
  pilA2 Legionella pneumophila strain ERS1305867

41.549

98.611

0.41
  pilE Neisseria gonorrhoeae strain FA1090

33.951

100

0.382
  pilA Vibrio parahaemolyticus RIMD 2210633

39.286

97.222

0.382
  pilE Neisseria gonorrhoeae MS11

41.538

90.278

0.375