Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KGP25_RS20195 Genome accession   NZ_CP074170
Coordinates   4183860..4184840 (-) Length   326 a.a.
NCBI ID   WP_223539538.1    Uniprot ID   -
Organism   Enterobacter sp. JBIWA003     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4178860..4189840
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KGP25_RS20165 (KGP25_20050) - 4179735..4180232 (+) 498 WP_021242073.1 SprT family zinc-dependent metalloprotease -
  KGP25_RS20170 (KGP25_20055) endA 4180327..4181034 (+) 708 WP_021242074.1 deoxyribonuclease I -
  KGP25_RS20175 (KGP25_20060) rsmE 4181087..4181818 (+) 732 WP_021242075.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  KGP25_RS20180 (KGP25_20065) gshB 4181838..4182785 (+) 948 WP_023326040.1 glutathione synthase -
  KGP25_RS20185 (KGP25_20070) - 4182873..4183433 (+) 561 WP_008499752.1 YqgE/AlgH family protein -
  KGP25_RS20190 (KGP25_20075) ruvX 4183433..4183849 (+) 417 WP_021242076.1 Holliday junction resolvase RuvX -
  KGP25_RS20195 (KGP25_20080) pilT 4183860..4184840 (-) 981 WP_223539538.1 type IV pilus twitching motility protein PilT Machinery gene
  KGP25_RS20200 (KGP25_20085) - 4184858..4185559 (+) 702 WP_223539539.1 YggS family pyridoxal phosphate-dependent enzyme -
  KGP25_RS20205 (KGP25_20090) - 4185581..4186147 (+) 567 WP_045909933.1 YggT family protein -
  KGP25_RS20210 (KGP25_20095) yggU 4186144..4186440 (+) 297 WP_045909934.1 DUF167 family protein YggU -
  KGP25_RS20215 (KGP25_20100) - 4186444..4187037 (+) 594 WP_223539540.1 XTP/dITP diphosphatase -
  KGP25_RS20220 (KGP25_20105) hemW 4187030..4188172 (+) 1143 WP_223539541.1 radical SAM family heme chaperone HemW -
  KGP25_RS20225 (KGP25_20110) - 4188236..4188574 (+) 339 WP_196365307.1 endonuclease domain-containing protein -
  KGP25_RS20230 (KGP25_20115) - 4188657..4189373 (-) 717 WP_008499762.1 DUF2884 domain-containing protein -
  KGP25_RS20235 (KGP25_20120) - 4189430..4189756 (-) 327 WP_003862421.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35481.42 Da        Isoelectric Point: 6.6964

>NTDB_id=563758 KGP25_RS20195 WP_223539538.1 4183860..4184840(-) (pilT) [Enterobacter sp. JBIWA003]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRTGRLEPAPFPPPDVGALLKAWLNDEQQGIWWASGQVDFAATVAGGQRLRG
SAFKQMRGVSVTLRLLPRTCPQLASLGAPRAIPELLTNESGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
FIYQSERCLIQQREIGQHSPSFADALRSALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLLPDLQGGRVALYELLVNTAAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSQAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=563758 KGP25_RS20195 WP_223539538.1 4183860..4184840(-) (pilT) [Enterobacter sp. JBIWA003]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCCCG
CTGGCGCAGAACAGGCCGCCTTGAACCGGCACCGTTTCCTCCTCCCGACGTCGGGGCGTTGTTAAAAGCGTGGCTCAACG
ATGAGCAGCAGGGCATATGGTGGGCCAGCGGGCAGGTGGATTTTGCCGCGACGGTGGCGGGAGGTCAGCGCCTGCGCGGC
AGTGCCTTTAAGCAGATGCGCGGTGTCTCTGTGACGCTACGGCTGTTGCCGCGTACCTGTCCGCAGCTCGCTTCACTGGG
TGCGCCGAGGGCCATCCCGGAACTGTTAACCAATGAGTCCGGGTTGATTCTTGTGACGGGGGCGACCGGCAGCGGTAAAT
CTACGACGCTGGCGGCGATGGTCGATTTTCTCAACCACCATACTGACGGCCATATCCTCACGCTCGAAGATCCGGTGGAA
TTTATCTACCAGAGCGAGCGTTGCCTGATCCAGCAGCGGGAGATTGGTCAGCACAGCCCGTCATTTGCCGACGCGCTGCG
CAGCGCCTTACGCCAGGATCCTGACGTGATTTTGCTCGGGGAGCTGCGCGACAGCGAAACGATCCGCCTGGCGCTAACGG
CAGCAGAGACCGGGCATCTGGTGCTGGCGACCCTGCATACGCGCGGGGCATCGCAGGCGATTGAGCGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCCGGCAGCCTGCGGGCGGTGCTGGCGCAGAAACTGCTTCC
CGATCTTCAGGGCGGGCGCGTCGCGCTGTATGAACTGCTGGTGAATACCGCCGCGGCGGCGAATCTCATTCGTGAAGGGA
AAACGTGGCAACTTCCCGGCATCATTCAAACCGGTCAGCAGGCAGGAATGCAGAACTTTGACCAGAGCCAGGCGGAGAGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(75-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.765

100

0.509
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.765

100

0.509
  pilT Legionella pneumophila strain ERS1305867

49.068

98.773

0.485
  pilT Legionella pneumophila strain Lp02

49.068

98.773

0.485
  pilT Neisseria meningitidis 8013

48.438

98.16

0.475
  pilT Acinetobacter baylyi ADP1

47.401

100

0.475
  pilT Pseudomonas stutzeri DSM 10701

48.896

97.239

0.475
  pilT Neisseria gonorrhoeae MS11

48.125

98.16

0.472
  pilT Acinetobacter baumannii D1279779

47.962

97.853

0.469
  pilT Acinetobacter baumannii strain A118

47.962

97.853

0.469
  pilT Acinetobacter nosocomialis M2

47.962

97.853

0.469
  pilT Pseudomonas aeruginosa PAK

47.95

97.239

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.34

97.546

0.433
  pilU Pseudomonas stutzeri DSM 10701

39.091

100

0.396
  pilU Vibrio cholerae strain A1552

39.498

97.853

0.386