Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CFSAN001992_RS18310 Genome accession   NC_020307
Coordinates   3744330..3745310 (+) Length   326 a.a.
NCBI ID   WP_001527996.1    Uniprot ID   A0A5Y8A9G2
Organism   Salmonella enterica subsp. enterica serovar Javiana str. CFSAN001992     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3739330..3750310
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CFSAN001992_RS18280 (CFSAN001992_18050) - 3739908..3740915 (+) 1008 WP_015406291.1 DUF1202 family protein -
  CFSAN001992_RS18285 (CFSAN001992_18055) hemW 3741006..3742142 (-) 1137 WP_001527976.1 radical SAM family heme chaperone HemW -
  CFSAN001992_RS18290 (CFSAN001992_18060) - 3742135..3742728 (-) 594 WP_001528003.1 XTP/dITP diphosphatase -
  CFSAN001992_RS18295 (CFSAN001992_18065) yggU 3742736..3743026 (-) 291 WP_001277203.1 DUF167 family protein YggU -
  CFSAN001992_RS18300 (CFSAN001992_18070) - 3743023..3743589 (-) 567 WP_001094848.1 YggT family protein -
  CFSAN001992_RS18305 (CFSAN001992_18075) - 3743608..3744312 (-) 705 WP_000997790.1 YggS family pyridoxal phosphate-dependent enzyme -
  CFSAN001992_RS18310 (CFSAN001992_18080) pilT 3744330..3745310 (+) 981 WP_001527996.1 type IV pilus twitching motility protein PilT Machinery gene
  CFSAN001992_RS18315 (CFSAN001992_18085) - 3745442..3746128 (+) 687 WP_001527920.1 IclR family transcriptional regulator -
  CFSAN001992_RS18320 (CFSAN001992_18090) ruvX 3746175..3746591 (-) 417 WP_000017096.1 Holliday junction resolvase RuvX -
  CFSAN001992_RS18325 (CFSAN001992_18095) - 3746591..3747154 (-) 564 WP_001053171.1 YqgE/AlgH family protein -
  CFSAN001992_RS18330 (CFSAN001992_18100) gshB 3747370..3748317 (-) 948 WP_000593248.1 glutathione synthase -
  CFSAN001992_RS18335 (CFSAN001992_18105) rsmE 3748337..3749068 (-) 732 WP_001222488.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  CFSAN001992_RS18340 (CFSAN001992_18110) endA 3749145..3749852 (-) 708 WP_001527975.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36094.31 Da        Isoelectric Point: 7.5355

>NTDB_id=56263 CFSAN001992_RS18310 WP_001527996.1 3744330..3745310(+) (pilT) [Salmonella enterica subsp. enterica serovar Javiana str. CFSAN001992]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMEIAPFTAPDVDRLLLDWLNDAQQYQWRAHGQLDFAVSLSGTRRLRA
SAFTHQQGTSLALRLLPERCPDLAEIQTPPIVPALLASENGLILVTGATGCGKSTTLAAMVGHLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=56263 CFSAN001992_RS18310 WP_001527996.1 3744330..3745310(+) (pilT) [Salmonella enterica subsp. enterica serovar Javiana str. CFSAN001992]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGATCGCGCCTTTTACCGCGCCAGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG
ACGCGCAACAATATCAGTGGCGAGCGCACGGCCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTACGCGCC
AGTGCGTTCACACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGCGTTGTCCTGATCTGGCGGAAATCCA
GACGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCTACTGGCTGCGGGAAGT
CCACCACGCTGGCGGCGATGGTGGGACATCTTAATCAACATGCCGATAAACACATCCTGACGCTGGAAGATCCTATTGAG
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCGGCGTTGCGTGAAGATCCCGATGTCATTTTGCTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGTGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGGTACGCAGTCAACTGGCGGGTAGTCTGCGCGCTGTACTATCGCAAAAACTGGAGGT
CGACAGACAGGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGCGCAGGGACGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5Y8A9G2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.624

100

0.488
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.624

100

0.488
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Acinetobacter baumannii strain A118

44.954

100

0.451
  pilT Acinetobacter baumannii D1279779

44.954

100

0.451
  pilT Acinetobacter baylyi ADP1

44.954

100

0.451
  pilT Pseudomonas aeruginosa PAK

44.954

100

0.451
  pilT Pseudomonas stutzeri DSM 10701

44.648

100

0.448
  pilT Acinetobacter nosocomialis M2

44.648

100

0.448
  pilT Legionella pneumophila strain Lp02

44.654

97.546

0.436
  pilT Legionella pneumophila strain ERS1305867

44.654

97.546

0.436
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

38.558

97.853

0.377
  pilU Pseudomonas stutzeri DSM 10701

36.336

100

0.371

Multiple sequence alignment