Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GHH_RS13320 Genome accession   NC_020210
Coordinates   2689544..2690599 (-) Length   351 a.a.
NCBI ID   WP_041470558.1    Uniprot ID   A0A4V1NTI1
Organism   Geobacillus sp. GHH01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2684544..2695599
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GHH_RS13295 (GHH_c27010) - 2685127..2685546 (-) 420 WP_015375506.1 type II secretion system protein -
  GHH_RS19560 (GHH_c27020) - 2685775..2685957 (+) 183 Protein_2626 RNA-guided endonuclease TnpB family protein -
  GHH_RS13305 (GHH_c27030) - 2686060..2687194 (+) 1135 Protein_2627 IS4-like element IS5377 family transposase -
  GHH_RS13310 (GHH_c27040) - 2687208..2688281 (+) 1074 Protein_2628 RNA-guided endonuclease InsQ/TnpB family protein -
  GHH_RS13315 (GHH_c27050) - 2688346..2689557 (-) 1212 WP_015375510.1 type II secretion system F family protein -
  GHH_RS13320 (GHH_c27060) pilT 2689544..2690599 (-) 1056 WP_041470558.1 type IV pilus twitching motility protein PilT Machinery gene
  GHH_RS13325 (GHH_c27070) - 2690612..2692276 (-) 1665 WP_015375512.1 GspE/PulE family protein -
  GHH_RS13330 (GHH_c27080) - 2692273..2693643 (-) 1371 WP_015375513.1 VanW family protein -
  GHH_RS13335 (GHH_c27090) - 2693660..2694439 (-) 780 WP_015375514.1 PRC-barrel domain-containing protein -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 38423.45 Da        Isoelectric Point: 6.8887

>NTDB_id=55844 GHH_RS13320 WP_041470558.1 2689544..2690599(-) (pilT) [Geobacillus sp. GHH01]
MKEKLEALLRAAFECRASDVHLTVGAPPIFRVNGELRAYGHERLHPEETEQMAKAVIPAPLWQQFQETGELDFSYGIAGV
SRFRLNVFKQRSCVSLAVRLIPVRIPTLEELGLPDVLKRIAEKPHGLVLVTGPTGSGKSTTLAAMIDYMNKTMSKHIITL
EDPIEYLHKHGKSIIDQREVGFDTGSFAAGLRAALRQDPDVILVGEMRDLETIQTAITAAETGHLVLGTLHTASAPAAIE
RMIDVFPPAQQAQIRLQLASVLVAIIAQRLFPNRHKTGRTAALEILVNNAAVANLIRNEKEHQIINIMQTSRSLGMQTME
MSVKELAAAGRIDPLAAEVYLAGERGDDGAV

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=55844 GHH_RS13320 WP_041470558.1 2689544..2690599(-) (pilT) [Geobacillus sp. GHH01]
ATGAAGGAAAAGCTTGAGGCGTTATTGCGCGCGGCCTTTGAATGCCGCGCTTCCGACGTGCATTTAACCGTCGGCGCGCC
GCCGATTTTTCGCGTGAACGGGGAGTTGAGAGCTTACGGCCATGAGCGCCTGCACCCGGAGGAAACAGAGCAAATGGCGA
AAGCCGTCATTCCGGCGCCGCTTTGGCAGCAGTTTCAGGAAACCGGAGAGCTTGATTTTTCGTATGGCATTGCCGGCGTT
TCCCGCTTTCGCTTAAACGTGTTTAAACAGCGGTCATGCGTGTCGCTCGCCGTGCGCCTCATCCCGGTGCGCATCCCGAC
GCTGGAGGAACTTGGCCTGCCGGATGTTTTAAAGCGCATCGCCGAAAAGCCGCACGGACTTGTGCTTGTCACCGGACCGA
CCGGCAGCGGCAAATCGACGACATTGGCGGCGATGATCGACTATATGAACAAAACGATGTCGAAACATATCATCACGCTG
GAAGATCCAATCGAGTATTTGCATAAACACGGGAAATCGATCATCGACCAACGCGAGGTGGGATTCGACACTGGCAGTTT
TGCCGCCGGATTGCGCGCTGCGCTTCGTCAAGATCCAGACGTCATTTTAGTCGGGGAAATGCGCGATTTGGAAACAATCC
AAACGGCAATCACCGCCGCGGAAACGGGCCATTTAGTGCTTGGCACGCTTCATACGGCGAGCGCGCCGGCGGCGATTGAG
CGGATGATTGACGTCTTTCCGCCGGCCCAGCAGGCGCAAATCCGGCTGCAGCTTGCTTCCGTGCTCGTGGCCATCATCGC
TCAGCGCTTGTTTCCAAACAGACACAAAACCGGGCGGACGGCAGCGCTGGAAATTTTAGTGAACAATGCGGCCGTCGCCA
ACTTAATCCGCAACGAAAAAGAACATCAAATCATCAACATTATGCAAACGAGCCGCAGCCTCGGCATGCAGACGATGGAA
ATGAGCGTTAAGGAACTTGCAGCCGCTGGACGCATCGATCCGCTGGCGGCTGAAGTGTATTTGGCAGGGGAGCGTGGGGA
CGATGGCGCAGTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4V1NTI1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.994

89.174

0.481
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.08

92.877

0.456
  pilT Vibrio cholerae strain A1552

49.08

92.877

0.456
  pilT Neisseria gonorrhoeae MS11

47.605

95.157

0.453
  pilT Neisseria meningitidis 8013

47.605

95.157

0.453
  pilT Pseudomonas aeruginosa PAK

49.226

92.023

0.453
  pilT Acinetobacter baylyi ADP1

48.16

92.877

0.447
  pilT Acinetobacter baumannii strain A118

47.273

94.017

0.444
  pilT Acinetobacter baumannii D1279779

47.273

94.017

0.444
  pilT Pseudomonas stutzeri DSM 10701

47.988

92.023

0.442
  pilT Acinetobacter nosocomialis M2

46.97

94.017

0.442
  pilT Legionella pneumophila strain Lp02

47.962

90.883

0.436
  pilT Legionella pneumophila strain ERS1305867

47.962

90.883

0.436
  pilU Pseudomonas stutzeri DSM 10701

44.073

93.732

0.413
  pilU Vibrio cholerae strain A1552

40.845

100

0.413

Multiple sequence alignment