Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   KAQ61_RS14995 Genome accession   NZ_CP072916
Coordinates   3194287..3195423 (+) Length   378 a.a.
NCBI ID   WP_154164176.1    Uniprot ID   A0A7X2I5C6
Organism   Comamonas aquatica strain NEB418     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3189287..3200423
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KAQ61_RS14975 (KAQ61_14975) - 3190658..3191062 (-) 405 WP_210929695.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  KAQ61_RS14980 (KAQ61_14980) - 3191088..3192239 (-) 1152 WP_210929696.1 aminotransferase class V-fold PLP-dependent enzyme -
  KAQ61_RS14985 (KAQ61_14985) - 3192410..3193129 (-) 720 WP_210929697.1 YggS family pyridoxal phosphate-dependent enzyme -
  KAQ61_RS14990 (KAQ61_14990) pilT 3193219..3194262 (+) 1044 WP_043383872.1 type IV pilus twitching motility protein PilT Machinery gene
  KAQ61_RS14995 (KAQ61_14995) pilU 3194287..3195423 (+) 1137 WP_154164176.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KAQ61_RS15000 (KAQ61_15000) - 3195487..3196146 (-) 660 WP_042417405.1 BON domain-containing protein -
  KAQ61_RS15005 (KAQ61_15005) - 3196146..3196742 (-) 597 WP_210929698.1 SIS domain-containing protein -
  KAQ61_RS15010 (KAQ61_15010) - 3196857..3197255 (-) 399 WP_042417409.1 YraN family protein -
  KAQ61_RS15015 (KAQ61_15015) rsmI 3197255..3198178 (+) 924 WP_210929699.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  KAQ61_RS15020 (KAQ61_15020) - 3198288..3199538 (-) 1251 WP_210929700.1 Hsp70 family protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42196.75 Da        Isoelectric Point: 6.4338

>NTDB_id=556871 KAQ61_RS14995 WP_154164176.1 3194287..3195423(+) (pilU) [Comamonas aquatica strain NEB418]
MERDQASKFINDLLKLMVARKGSDLFITAEFPPAIKVDGKVTRLMDQPLVPTHTLMLARSIMSDKQVAEFERTKECNFAI
SPAGIGRFRVNAFIQQGRVGMVLRTIPADIPTIDGMGLPQVLKEVAMTKRGLCIFVGATGSGKSTSLAAMVDWRNEHSEG
HIVTVEDPIEFVHPHKNCIITQREVGLDTDSWEAALKNSLRQAPDVILMGEIRDRETMEHAIVFSETGHLCLATLHANSA
NQALDRIVNFFPEERREQLLMDLSLNLRAMISQRLLPKEGGQGRVAALEVMLNTPLISDLIAKGDVVEIKEIMKKSRELG
MQTFDQSLFDLYESGAVMYEDALRNADSVNDLRLQIKLHSKRAHNTDLAAGTEHFRIL

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=556871 KAQ61_RS14995 WP_154164176.1 3194287..3195423(+) (pilU) [Comamonas aquatica strain NEB418]
ATGGAACGCGATCAGGCCAGCAAATTCATCAATGACCTCCTCAAGCTGATGGTGGCGCGCAAGGGCAGCGACCTGTTCAT
CACCGCCGAATTTCCGCCCGCCATCAAGGTCGACGGCAAGGTCACACGGCTGATGGACCAGCCGCTGGTGCCCACGCACA
CCCTGATGCTGGCACGCTCCATCATGAGCGACAAGCAGGTGGCTGAATTCGAGCGCACCAAGGAGTGCAACTTCGCCATC
TCACCCGCCGGCATCGGGCGTTTTCGCGTCAACGCCTTCATCCAGCAGGGCCGCGTGGGTATGGTGCTGCGGACCATTCC
GGCCGACATCCCCACCATCGACGGCATGGGCCTGCCCCAGGTGCTGAAGGAAGTGGCCATGACCAAGCGCGGCCTGTGCA
TCTTTGTGGGGGCCACCGGGTCGGGCAAGTCGACCTCGCTGGCGGCCATGGTGGACTGGCGCAACGAACACTCCGAAGGG
CACATCGTCACGGTGGAAGACCCCATCGAATTCGTGCACCCCCACAAGAACTGCATCATCACCCAGCGCGAAGTGGGTCT
GGACACCGACAGCTGGGAAGCGGCACTCAAGAACTCGCTGCGCCAGGCGCCCGACGTGATCCTGATGGGCGAAATCCGCG
ACCGTGAAACCATGGAGCACGCCATCGTGTTCTCCGAAACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAGTGCC
AACCAGGCGCTGGACCGCATCGTCAACTTCTTCCCGGAAGAGCGCCGCGAGCAACTGCTGATGGACCTGTCGCTGAACCT
GCGCGCCATGATCTCGCAGCGCCTGCTGCCCAAAGAAGGCGGCCAGGGCCGTGTGGCCGCGCTGGAGGTGATGCTCAACA
CCCCACTGATCTCGGACCTGATCGCCAAGGGCGACGTGGTGGAAATCAAGGAAATCATGAAGAAAAGTCGCGAGCTGGGC
ATGCAGACCTTCGACCAGTCGCTGTTCGACCTCTACGAAAGCGGCGCCGTCATGTACGAGGATGCGCTGCGCAACGCCGA
CTCGGTGAACGACCTGCGCCTGCAGATCAAGCTGCACAGCAAACGTGCCCACAACACCGACCTGGCGGCGGGTACCGAGC
ACTTCCGCATCCTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7X2I5C6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.219

95.503

0.585
  pilU Acinetobacter baylyi ADP1

59.143

92.593

0.548
  pilU Vibrio cholerae strain A1552

55.172

92.063

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.401

89.153

0.405
  pilT Acinetobacter baylyi ADP1

45.181

87.831

0.397
  pilT Pseudomonas aeruginosa PAK

44.214

89.153

0.394
  pilT Pseudomonas stutzeri DSM 10701

43.695

90.212

0.394
  pilT Acinetobacter baumannii strain A118

44.144

88.095

0.389
  pilT Acinetobacter baumannii D1279779

44.144

88.095

0.389
  pilT Acinetobacter nosocomialis M2

44.144

88.095

0.389
  pilT Legionella pneumophila strain Lp02

45.031

85.185

0.384
  pilT Legionella pneumophila strain ERS1305867

45.031

85.185

0.384
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.598

86.772

0.378
  pilT Vibrio cholerae strain A1552

43.598

86.772

0.378