Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   J9870_RS24865 Genome accession   NZ_CP072913
Coordinates   5724668..5725078 (-) Length   136 a.a.
NCBI ID   WP_210640979.1    Uniprot ID   -
Organism   Pseudomonas sp. Tri1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5719668..5730078
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J9870_RS24850 - 5722264..5722560 (+) 297 WP_210640977.1 DUF2845 domain-containing protein -
  J9870_RS24855 - 5722663..5723625 (-) 963 WP_210639263.1 transposase -
  J9870_RS24860 - 5724045..5724398 (-) 354 WP_003205664.1 BON domain-containing protein -
  J9870_RS24865 pilA 5724668..5725078 (-) 411 WP_210640979.1 pilin Machinery gene
  J9870_RS24870 pilB 5725303..5727003 (+) 1701 WP_210640980.1 type IV-A pilus assembly ATPase PilB Machinery gene
  J9870_RS24875 pilC 5727006..5728223 (+) 1218 WP_210640982.1 type II secretion system F family protein Machinery gene
  J9870_RS24880 pilD 5728225..5729094 (+) 870 WP_210640984.1 A24 family peptidase Machinery gene
  J9870_RS24885 coaE 5729106..5729729 (+) 624 WP_210640986.1 dephospho-CoA kinase -
  J9870_RS24890 yacG 5729726..5729932 (+) 207 WP_003205675.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 13832.97 Da        Isoelectric Point: 8.1178

>NTDB_id=556819 J9870_RS24865 WP_210640979.1 5724668..5725078(-) (pilA) [Pseudomonas sp. Tri1]
MNKHKGFTLIELLIVVAIIGILATFALPQYSKYQARAKATAGLGEISALKVPYEDMMNQGTNPTADNMNITTPTSNCAIA
VTGTASAGTGTIVCTLSNAPAAVAGKTITLSRDAAGAWTCASNAAKEFLPAGCPGV

Nucleotide


Download         Length: 411 bp        

>NTDB_id=556819 J9870_RS24865 WP_210640979.1 5724668..5725078(-) (pilA) [Pseudomonas sp. Tri1]
ATGAATAAGCACAAGGGTTTTACGCTGATCGAGCTGCTGATCGTCGTGGCGATCATCGGCATTCTGGCAACGTTTGCGTT
GCCGCAATATTCCAAGTACCAGGCGCGAGCCAAGGCTACGGCCGGGCTTGGGGAAATTTCTGCGTTGAAGGTACCGTACG
AGGACATGATGAATCAGGGAACTAACCCAACAGCGGACAACATGAACATCACGACGCCTACCAGCAACTGCGCAATAGCA
GTCACGGGTACTGCTTCGGCAGGCACAGGCACCATCGTTTGTACCCTCTCAAATGCTCCTGCTGCTGTAGCGGGTAAGAC
GATCACCCTTAGCCGAGACGCCGCCGGTGCCTGGACATGCGCATCTAACGCTGCCAAGGAATTCCTGCCGGCTGGCTGCC
CTGGCGTCTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-133)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

48.175

100

0.485
  pilA/pilAI Pseudomonas stutzeri DSM 10701

44.118

100

0.441
  comP Acinetobacter baylyi ADP1

38.667

100

0.426
  pilA Pseudomonas aeruginosa PAK

39.189

100

0.426
  pilA Ralstonia pseudosolanacearum GMI1000

36.601

100

0.412
  pilA/pilA1 Eikenella corrodens VA1

36.735

100

0.397
  pilA/pilAII Pseudomonas stutzeri DSM 10701

40

99.265

0.397
  pilA2 Legionella pneumophila str. Paris

36.552

100

0.39
  pilA2 Legionella pneumophila strain ERS1305867

35.862

100

0.382
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36.17

100

0.375
  pilA Vibrio cholerae strain A1552

36.17

100

0.375
  pilA Vibrio cholerae C6706

36.17

100

0.375