Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   J9870_RS24870 Genome accession   NZ_CP072913
Coordinates   5725303..5727003 (+) Length   566 a.a.
NCBI ID   WP_210640980.1    Uniprot ID   -
Organism   Pseudomonas sp. Tri1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5720303..5732003
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J9870_RS24850 - 5722264..5722560 (+) 297 WP_210640977.1 DUF2845 domain-containing protein -
  J9870_RS24855 - 5722663..5723625 (-) 963 WP_210639263.1 transposase -
  J9870_RS24860 - 5724045..5724398 (-) 354 WP_003205664.1 BON domain-containing protein -
  J9870_RS24865 pilA 5724668..5725078 (-) 411 WP_210640979.1 pilin Machinery gene
  J9870_RS24870 pilB 5725303..5727003 (+) 1701 WP_210640980.1 type IV-A pilus assembly ATPase PilB Machinery gene
  J9870_RS24875 pilC 5727006..5728223 (+) 1218 WP_210640982.1 type II secretion system F family protein Machinery gene
  J9870_RS24880 pilD 5728225..5729094 (+) 870 WP_210640984.1 A24 family peptidase Machinery gene
  J9870_RS24885 coaE 5729106..5729729 (+) 624 WP_210640986.1 dephospho-CoA kinase -
  J9870_RS24890 yacG 5729726..5729932 (+) 207 WP_003205675.1 DNA gyrase inhibitor YacG -
  J9870_RS24895 - 5729979..5730194 (-) 216 WP_210640988.1 hypothetical protein -
  J9870_RS24900 - 5730258..5730944 (-) 687 WP_210640990.1 energy-coupling factor ABC transporter permease -
  J9870_RS24905 - 5731019..5731432 (-) 414 WP_210640992.1 tautomerase family protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62819.81 Da        Isoelectric Point: 5.5407

>NTDB_id=556820 J9870_RS24870 WP_210640980.1 5725303..5727003(+) (pilB) [Pseudomonas sp. Tri1]
MNDIALSGLTKQLVLAELLTEQSAQQAYQQAQHSRTPLVSYLVQNKLVQSRQVAEIASEHFGVALLDLNSLDKETQPTGL
VSEKLVRQHHALPLWRRGNKLFVGISDPTNHQAINDIQFSTGLTTEAILVEDDKLSDAIEKFFESSSTGLEGMGDVDLDG
LDIESIDEHKQDSIAGQDTDDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRVRTDGMLREVARPPIQLATRIAARL
KVMASLDISERRKPQDGRLKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPDQKDLYMAALKQPQG
LILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLIRLQNMGIPGFNIATAVHLIIAQRLARKLCNHCKRTIEVPEETLLKEGFPR
ERIGTFTIYEPVGCEQCNHGYKGRVGVYEVVKNTPELQRLIMAEGNSLEIDLQMRKDGFNDLRTSGLLKVMQGMTSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=556820 J9870_RS24870 WP_210640980.1 5725303..5727003(+) (pilB) [Pseudomonas sp. Tri1]
ATGAATGACATCGCCCTCAGCGGCCTGACCAAGCAATTGGTGCTGGCCGAACTGCTCACCGAGCAAAGTGCGCAGCAGGC
GTACCAACAGGCCCAGCACAGTCGCACGCCCCTGGTCAGTTATCTGGTACAGAACAAACTGGTCCAGAGCCGCCAGGTGG
CGGAGATCGCCTCGGAGCATTTTGGCGTCGCCCTGCTGGACCTCAACAGCCTGGACAAAGAGACCCAACCCACGGGCCTG
GTCAGCGAAAAACTGGTGCGCCAGCACCACGCCCTGCCACTGTGGCGGCGTGGCAATAAATTGTTCGTGGGCATCTCCGA
CCCGACCAACCACCAGGCCATCAATGACATCCAGTTCAGCACCGGCCTGACCACCGAAGCCATCCTGGTGGAGGACGACA
AGCTCAGCGACGCCATCGAGAAGTTCTTCGAATCCAGCAGCACGGGCCTGGAAGGTATGGGTGATGTCGACCTCGATGGC
CTGGACATCGAGTCGATCGACGAGCACAAGCAGGATTCCATCGCTGGCCAGGACACCGACGACGCGCCGGTGGTGCGGTT
CGTCAACAAGATGCTGCTGGACGCGATCAAGGGCGGCTCCTCCGATTTGCACTTCGAGCCCTATGAAAAAATCTACCGGG
TGCGGGTGCGTACCGATGGCATGTTGCGGGAGGTGGCCCGGCCACCGATCCAGTTGGCCACCCGTATCGCCGCACGCCTC
AAGGTGATGGCCAGCCTCGATATCTCCGAACGGCGCAAACCCCAGGACGGGCGCCTGAAAATGCGCCTGTCGAAAAGCAA
ATCGATCGATTTCCGGGTCAACACCCTGCCCACCCTCTGGGGCGAAAAAGTGGTGATCCGGATTCTCGACCCGTCCAGCG
CACAAATGGGCATCGATGCCCTGGGCTACGAACCGGATCAGAAAGACCTGTACATGGCGGCCCTCAAACAACCACAAGGC
TTGATCCTGGTGACCGGGCCGACCGGCTCGGGCAAGACCGTGTCGCTGTACACCGGTCTGAATATCCTCAACACCGTGGA
CATCAACATTTCCACCGCCGAAGACCCGGTGGAGATCAACATGGAAGGCATCAACCAGGTCAACGTGAACCCACGCCAGG
GGCTGGATTTCGCTCAAGCCCTACGCTCGTTCCTGCGCCAGGACCCGGATGTGATCATGGTCGGCGAGATCCGCGACCTG
GAAACCGCCGAAATCGCCATCAAGGCAGCACAGACCGGGCACCTGGTGCTCTCCACGCTGCACACCAACAGCGCCGCCGA
AACCTTGATTCGCCTGCAGAACATGGGCATTCCGGGCTTCAATATCGCCACCGCTGTACACCTGATCATCGCCCAACGGC
TGGCGCGCAAGCTGTGCAACCACTGCAAGAGAACCATCGAGGTGCCTGAGGAGACCCTGCTCAAGGAAGGTTTCCCCCGG
GAACGCATCGGCACATTCACGATCTATGAGCCGGTTGGATGCGAACAGTGCAACCACGGTTACAAAGGGCGCGTGGGGGT
TTATGAAGTGGTCAAGAACACACCTGAGCTGCAACGGTTGATCATGGCCGAGGGCAACTCGCTGGAAATCGACCTGCAAA
TGCGCAAGGACGGTTTCAATGACCTGCGCACATCGGGGCTGCTCAAGGTGATGCAGGGCATGACCAGCCTCGAGGAAATC
AACCGGGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.834

100

0.588
  pilB Acinetobacter baumannii D1279779

56.915

99.647

0.567
  pilB Legionella pneumophila strain ERS1305867

55.556

100

0.557
  pilB Vibrio cholerae strain A1552

50.619

99.823

0.505
  pilB Vibrio campbellii strain DS40M4

48.759

99.647

0.486
  pilB Vibrio parahaemolyticus RIMD 2210633

49.455

97.173

0.481
  pilF Neisseria gonorrhoeae MS11

48.313

99.47

0.481