Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   J8L76_RS06085 Genome accession   NZ_CP072875
Coordinates   1314276..1315412 (+) Length   378 a.a.
NCBI ID   WP_216131146.1    Uniprot ID   A0A975SPE1
Organism   Azospira inquinata strain Azo-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1309276..1320412
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J8L76_RS06055 (J8L76_06055) - 1309648..1310364 (+) 717 WP_216131128.1 PAS domain-containing transcriptional regulator -
  J8L76_RS06060 (J8L76_06060) - 1310468..1310764 (-) 297 WP_216131131.1 DUF167 domain-containing protein -
  J8L76_RS06065 (J8L76_06065) - 1310764..1311336 (-) 573 WP_216131134.1 YggT family protein -
  J8L76_RS06070 (J8L76_06070) proC 1311524..1312342 (-) 819 WP_216131137.1 pyrroline-5-carboxylate reductase -
  J8L76_RS06075 (J8L76_06075) - 1312339..1313061 (-) 723 WP_216131140.1 YggS family pyridoxal phosphate-dependent enzyme -
  J8L76_RS06080 (J8L76_06080) pilT 1313179..1314222 (+) 1044 WP_216131143.1 type IV pilus twitching motility protein PilT Machinery gene
  J8L76_RS06085 (J8L76_06085) pilU 1314276..1315412 (+) 1137 WP_216131146.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  J8L76_RS06090 (J8L76_06090) - 1315452..1315754 (+) 303 WP_216131149.1 hypothetical protein -
  J8L76_RS06095 (J8L76_06095) - 1315793..1316665 (-) 873 WP_216131152.1 acid phosphatase -
  J8L76_RS06100 (J8L76_06100) - 1316736..1317914 (-) 1179 WP_216131155.1 THUMP domain-containing class I SAM-dependent RNA methyltransferase -
  J8L76_RS06105 (J8L76_06105) - 1317999..1318421 (-) 423 WP_216131158.1 CopD family protein -
  J8L76_RS06110 (J8L76_06110) - 1318470..1319354 (-) 885 WP_216131161.1 SDR family oxidoreductase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41909.19 Da        Isoelectric Point: 6.5047

>NTDB_id=556489 J8L76_RS06085 WP_216131146.1 1314276..1315412(+) (pilU) [Azospira inquinata strain Azo-2]
MERDQALNFMHDLLRLMVSKKGSDLFITAGFPPAIKVDGRLTPVSNQTLSAQHTAELARAIMNDRQAAEFEAHKECNFAI
APAGIGRFRVNAFVQMGRIGVVCRTINTTIPTLEALGLPPVLKDVAMTKRGLVLFVGGTGSGKSTSLAAMIGYRNENSYG
HIITIEDPVEYVHEHRNCIITQREVGVDTDSWETALKNTLRQAPDVILIGEIRDRETMEHAIAFSETGHLAMGTLHANST
NQALDRIINFFPEEKRQQLLMDLSLNLRGLVSQRLIPLKDGKGRCAAIEIMLNSPLISDLIFKGEVAEIKEIMKKSRELG
MQTFDQSLFELYEAGKISYEDALRNADSLNDLRLQIKLNGKESKDRDLSAGIGHLDIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=556489 J8L76_RS06085 WP_216131146.1 1314276..1315412(+) (pilU) [Azospira inquinata strain Azo-2]
ATGGAGCGGGATCAGGCGCTTAATTTCATGCACGATTTGCTGCGCCTCATGGTCAGCAAAAAGGGCTCGGATTTGTTCAT
TACCGCCGGGTTTCCCCCGGCCATCAAGGTGGATGGCCGGCTCACCCCGGTCTCCAACCAGACCCTGTCGGCCCAGCACA
CGGCGGAACTGGCCCGGGCCATCATGAATGACCGCCAGGCAGCGGAATTCGAAGCCCATAAGGAATGCAATTTCGCCATT
GCCCCCGCCGGCATTGGCCGTTTCCGGGTCAATGCCTTTGTCCAAATGGGGCGCATCGGGGTGGTCTGCCGGACCATCAA
CACTACCATTCCCACCCTGGAAGCCCTGGGGCTGCCGCCGGTCCTGAAGGACGTGGCCATGACCAAGCGGGGTCTGGTGC
TCTTCGTGGGGGGTACGGGCTCGGGGAAATCCACTTCCCTGGCGGCCATGATCGGCTACCGCAACGAAAACTCCTACGGC
CACATCATTACCATCGAAGACCCGGTGGAATATGTCCATGAGCACAGGAACTGCATCATCACCCAGCGGGAAGTGGGGGT
GGATACGGATTCCTGGGAAACGGCCCTGAAAAACACCCTGCGTCAGGCCCCGGATGTGATCCTGATTGGGGAAATCCGGG
ATCGGGAAACCATGGAACACGCCATCGCCTTTTCCGAAACCGGCCACTTGGCCATGGGCACTCTCCACGCCAATTCCACC
AATCAGGCCCTGGACCGGATCATCAACTTTTTCCCGGAAGAAAAGCGTCAGCAGCTGCTCATGGACCTGTCCCTCAATCT
CCGGGGCCTGGTCTCCCAGCGCCTGATTCCCCTGAAGGACGGCAAGGGGCGCTGCGCCGCCATCGAAATCATGCTCAACT
CCCCCCTCATTTCCGACCTGATTTTCAAGGGGGAAGTGGCGGAGATTAAGGAAATCATGAAAAAGTCCCGGGAACTGGGG
ATGCAGACCTTCGACCAGTCCCTGTTCGAGCTTTACGAAGCGGGGAAAATTTCCTACGAGGATGCCCTGCGCAATGCGGA
TTCCCTGAACGACCTGCGCCTCCAGATCAAGCTTAACGGCAAGGAATCCAAGGACCGGGATCTGTCCGCCGGCATCGGCC
ACCTGGATATTGTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(122-256)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.525

94.709

0.611
  pilU Acinetobacter baylyi ADP1

58.496

94.974

0.556
  pilU Vibrio cholerae strain A1552

57.185

90.212

0.516
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.672

88.624

0.405
  pilT Pseudomonas aeruginosa PAK

44.478

88.624

0.394
  pilT Legionella pneumophila strain Lp02

45.015

87.566

0.394
  pilT Legionella pneumophila strain ERS1305867

45.015

87.566

0.394
  pilT Acinetobacter baylyi ADP1

43.62

89.153

0.389
  pilT Acinetobacter nosocomialis M2

44.109

87.566

0.386
  pilT Acinetobacter baumannii D1279779

44.109

87.566

0.386
  pilT Acinetobacter baumannii strain A118

44.109

87.566

0.386
  pilT Pseudomonas stutzeri DSM 10701

42.687

88.624

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.727

87.302

0.373
  pilT Vibrio cholerae strain A1552

42.727

87.302

0.373
  pilT Neisseria meningitidis 8013

40.533

89.418

0.362