NTDB

Overview

Name	pilA2	Type	Machinery gene
Locus tag	J9260_RS17585	Genome accession	NZ_CP072793
Coordinates	3529853..3530287 (-)	Length	144 a.a.
NCBI ID	WP_281419405.1	Uniprot ID	-
Organism	Thiothrix unzii strain A1
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3524853..3535287

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
J9260_RS17570 (J9260_17565)	-	3525601..3526812 (-)	1212	WP_210218999.1	glycosyltransferase family 4 protein	-
J9260_RS17575 (J9260_17570)	-	3526814..3527758 (-)	945	WP_210219000.1	glycosyltransferase family 2 protein	-
J9260_RS17580 (J9260_17575)	-	3527715..3529721 (-)	2007	WP_210219001.1	hypothetical protein	-
J9260_RS17585 (J9260_17580)	pilA2	3529853..3530287 (-)	435	WP_281419405.1	pilin	Machinery gene
J9260_RS17590 (J9260_17585)	-	3530550..3531302 (+)	753	WP_210219002.1	ATP-binding protein	-
J9260_RS17595 (J9260_17590)	acs	3531453..3533402 (+)	1950	WP_210219003.1	acetate--CoA ligase	-
J9260_RS17600 (J9260_17595)	-	3533526..3533828 (-)	303	WP_210219004.1	DUF2288 domain-containing protein	-
J9260_RS17605 (J9260_17600)	-	3533896..3534984 (-)	1089	WP_210219005.1	glycosyltransferase	-

Sequence

Protein

Download Length: 144 a.a. Molecular weight: 14931.17 Da Isoelectric Point: 10.1068

                                >NTDB_id=555894 J9260_RS17585 WP_281419405.1 3529853..3530287(-) (pilA2) [Thiothrix unzii strain A1]

MSMKAKAQQGFTLIELMIVVAIIGILAAIAMPAYQDYTKRSHVTEGLNLAASAKSGVTEFFADRGAFPTSNVEAGLATAT

NIGGNSVRSVAVGAAGVITITYRTNVTPGATLLLTPTNSSGSITWSCKLNGTVAQKYRPSSCRT

Nucleotide

Download Length: 435 bp

                                >NTDB_id=555894 J9260_RS17585 WP_281419405.1 3529853..3530287(-) (pilA2) [Thiothrix unzii strain A1]

ATGAGCATGAAAGCAAAAGCCCAACAAGGCTTCACCCTGATCGAATTGATGATCGTTGTCGCCATCATCGGCATTCTGGC

AGCGATTGCCATGCCAGCTTACCAAGATTACACCAAGCGTTCACACGTTACTGAAGGTTTGAACTTGGCTGCCAGCGCAA

AATCTGGTGTTACTGAATTCTTTGCAGACCGTGGTGCATTCCCAACAAGCAACGTAGAAGCAGGCTTGGCTACCGCAACC

AACATCGGTGGTAACTCCGTACGTTCTGTTGCCGTAGGTGCCGCAGGTGTTATCACCATTACTTACCGCACTAACGTAAC

ACCCGGCGCAACTCTGCTGCTCACGCCAACCAATAGCAGTGGCTCTATCACTTGGAGCTGTAAATTAAACGGCACTGTAG

CGCAAAAATACCGTCCATCAAGCTGCCGGACATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(40-143)

Prokaryotic N-terminal methylation motif (N_methyl)

(6-31)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA2	Legionella pneumophila str. Paris	55.474	95.139	0.528
pilA2	Legionella pneumophila strain ERS1305867	55.474	95.139	0.528
pilA	Ralstonia pseudosolanacearum GMI1000	41.954	100	0.507
pilE	Neisseria elongata subsp. glycolytica ATCC 29315	37.566	100	0.493
comP	Acinetobacter baylyi ADP1	45.89	100	0.465
pilE	Neisseria gonorrhoeae MS11	42.308	100	0.458
pilA/pilA1	Eikenella corrodens VA1	42.208	100	0.451
pilE	Neisseria gonorrhoeae strain FA1090	41.935	100	0.451
pilA	Vibrio campbellii strain DS40M4	44.928	95.833	0.431
pilA/pilAI	Pseudomonas stutzeri DSM 10701	39.583	100	0.396
pilA	Vibrio cholerae strain A1552	38.095	100	0.389
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	38.095	100	0.389
pilA	Vibrio cholerae C6706	38.095	100	0.389
pilA	Pseudomonas aeruginosa PAK	34.839	100	0.375
pilA	Acinetobacter baumannii strain A118	38.849	96.528	0.375

Detailed information NTG0555894