Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   J5249_RS05650 Genome accession   NZ_CP072665
Coordinates   1129252..1130313 (-) Length   353 a.a.
NCBI ID   WP_120655514.1    Uniprot ID   -
Organism   Campylobacter fetus subsp. fetus strain CITCf02     
Function   pseudopilus biogenesis (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1124252..1135313
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J5249_RS05625 (J5249_01098) - 1124901..1125443 (-) 543 WP_111738207.1 DUF1882 domain-containing protein -
  J5249_RS05630 (J5249_01099) - 1125443..1126687 (-) 1245 WP_111738208.1 serine hydroxymethyltransferase -
  J5249_RS05635 (J5249_01100) lysS 1126684..1128195 (-) 1512 WP_225893286.1 lysine--tRNA ligase -
  J5249_RS05640 (J5249_01101) - 1128209..1128673 (-) 465 WP_002849768.1 Fur family transcriptional regulator -
  J5249_RS05645 (J5249_01102) - 1128677..1129252 (-) 576 WP_002849774.1 CvpA family protein -
  J5249_RS05650 (J5249_01103) pilT 1129252..1130313 (-) 1062 WP_120655514.1 type IV pilus twitching motility protein PilT Machinery gene
  J5249_RS05655 (J5249_01104) gatC 1130317..1130604 (-) 288 WP_002849777.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC -
  J5249_RS05660 (J5249_01105) - 1130787..1131386 (+) 600 WP_225893264.1 hypothetical protein -
  J5249_RS05665 (J5249_01106) - 1131389..1132396 (+) 1008 WP_038453751.1 L-seryl-tRNA selenium transferase -
  J5249_RS05670 (J5249_01107) - 1132403..1132729 (+) 327 WP_002849781.1 hypothetical protein -
  J5249_RS05675 (J5249_01108) - 1132716..1133348 (+) 633 WP_002849782.1 type III pantothenate kinase -
  J5249_RS05680 (J5249_01109) hisG 1133336..1133947 (+) 612 WP_010400265.1 ATP phosphoribosyltransferase -
  J5249_RS05685 (J5249_01110) brnQ 1133956..1135260 (+) 1305 WP_111738209.1 branched-chain amino acid transport system II carrier protein -

Sequence

Protein


Download         Length: 353 a.a.        Molecular weight: 38969.39 Da        Isoelectric Point: 6.3450

>NTDB_id=554908 J5249_RS05650 WP_120655514.1 1129252..1130313(-) (pilT) [Campylobacter fetus subsp. fetus strain CITCf02]
MNIETLLKTVMHNKASDLHLVGRSEPQIRIDGSLRPLEGKVLDGSDIENLCYTILTDAQKSELEENKDLDFALELPGIGR
FRGNCYYTMNGSLAAAFRMIPLNIPSLDDLNAPAIFKELVKKEKGLVLVTGGTGAGKSTTLAALLNEINITERKHIITIE
DPIEFVHQNKNSLFSHRNVGTDTKSFARALKYALREDPDIILVGDLRDPETISIAIAAAETGHLVFGTLHTNSAVQTINR
IVDSFNETEQAQVRNMLSVSLNAIISQTLVPKADNGRFAVHEVLINNHAISNLIRENKSHQIYSQIQLNQQNTGMQTQTQ
AMEKAVQMGFITKENAIRYSTNQQELAGKLGAI

Nucleotide


Download         Length: 1062 bp        

>NTDB_id=554908 J5249_RS05650 WP_120655514.1 1129252..1130313(-) (pilT) [Campylobacter fetus subsp. fetus strain CITCf02]
ATGAATATTGAAACTCTTTTAAAAACTGTGATGCATAACAAGGCGAGCGATCTACATTTAGTCGGTAGAAGTGAGCCTCA
AATCAGAATTGATGGTAGCTTAAGACCGCTTGAAGGTAAAGTTTTAGATGGAAGCGATATAGAAAATTTATGCTACACTA
TTTTAACTGATGCGCAAAAAAGCGAATTGGAAGAGAACAAGGATTTAGATTTTGCTCTAGAATTACCAGGAATAGGGCGT
TTTAGAGGTAATTGCTACTATACTATGAACGGAAGTTTAGCGGCTGCTTTTAGAATGATACCTCTAAATATACCAAGTCT
TGATGATCTTAATGCTCCGGCTATTTTTAAAGAGTTGGTAAAAAAAGAAAAAGGTCTTGTTCTTGTTACTGGAGGAACGG
GAGCTGGAAAATCGACGACTTTAGCAGCTTTGCTAAATGAGATAAATATTACTGAGCGAAAACATATAATCACCATAGAA
GATCCTATAGAATTTGTTCATCAAAATAAGAATTCTCTATTCTCTCATAGAAACGTAGGCACCGATACTAAATCGTTTGC
TAGGGCTTTGAAATACGCTCTTAGAGAAGATCCGGATATAATATTAGTAGGTGATTTAAGAGATCCGGAGACCATATCTA
TAGCTATAGCGGCCGCAGAAACAGGACACTTGGTATTTGGTACTTTGCACACAAACTCAGCCGTACAAACAATAAATAGA
ATAGTCGATAGTTTTAATGAGACAGAGCAGGCTCAAGTAAGAAATATGCTTAGCGTATCTCTAAATGCTATTATATCTCA
AACTCTTGTGCCAAAAGCAGATAACGGCAGATTTGCCGTTCATGAAGTTTTGATAAATAATCATGCAATTTCAAATTTGA
TCAGAGAAAATAAATCTCATCAAATTTACTCTCAAATACAATTAAATCAACAAAATACAGGTATGCAAACACAAACTCAA
GCGATGGAAAAGGCGGTGCAAATGGGTTTTATAACAAAAGAAAACGCTATAAGATATTCAACAAATCAGCAAGAATTAGC
TGGAAAGTTAGGTGCAATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria gonorrhoeae MS11

50.287

98.584

0.496
  pilT Neisseria meningitidis 8013

50

98.584

0.493
  pilT Legionella pneumophila strain Lp02

50.437

97.167

0.49
  pilT Legionella pneumophila strain ERS1305867

50.437

97.167

0.49
  pilT Vibrio cholerae strain A1552

51.964

93.768

0.487
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.964

93.768

0.487
  pilT Acinetobacter baumannii D1279779

49.565

97.734

0.484
  pilT Acinetobacter nosocomialis M2

49.565

97.734

0.484
  pilT Acinetobacter baumannii strain A118

49.565

97.734

0.484
  pilT Pseudomonas aeruginosa PAK

49.702

95.184

0.473
  pilT Acinetobacter baylyi ADP1

49.849

93.768

0.467
  pilT Pseudomonas stutzeri DSM 10701

48.81

95.184

0.465
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.092

98.584

0.405
  pilU Pseudomonas stutzeri DSM 10701

40

100

0.402
  pilU Acinetobacter baylyi ADP1

39.181

96.884

0.38