Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PCC7418_RS05280 Genome accession   NC_019779
Coordinates   1148573..1149685 (-) Length   370 a.a.
NCBI ID   WP_015225139.1    Uniprot ID   K9Y9W6
Organism   Halothece sp. PCC 7418     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1143573..1154685
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCC7418_RS05255 (PCC7418_1049) aroC 1143615..1144709 (-) 1095 WP_015225134.1 chorismate synthase -
  PCC7418_RS05260 (PCC7418_1050) - 1144917..1145447 (-) 531 WP_015225135.1 TIGR02281 family clan AA aspartic protease -
  PCC7418_RS05265 (PCC7418_1051) ald 1145578..1146660 (+) 1083 WP_015225136.1 alanine dehydrogenase -
  PCC7418_RS05270 (PCC7418_1052) - 1146719..1147288 (-) 570 WP_015225137.1 hypothetical protein -
  PCC7418_RS05275 (PCC7418_1053) - 1147314..1148534 (-) 1221 WP_015225138.1 type II secretion system F family protein -
  PCC7418_RS05280 (PCC7418_1054) pilT 1148573..1149685 (-) 1113 WP_015225139.1 type IV pilus twitching motility protein PilT Machinery gene
  PCC7418_RS05285 (PCC7418_1055) pilB/pilB1 1149914..1151938 (-) 2025 WP_015225140.1 GspE/PulE family protein Machinery gene
  PCC7418_RS05295 (PCC7418_1056) bchL 1152744..1153622 (+) 879 WP_015225141.1 ferredoxin:protochlorophyllide reductase (ATP-dependent) iron-sulfur ATP-binding protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40900.91 Da        Isoelectric Point: 7.7047

>NTDB_id=55053 PCC7418_RS05280 WP_015225139.1 1148573..1149685(-) (pilT) [Halothece sp. PCC 7418]
MDLMIEDLMEQLVEKGGSDMHIQAGAPVYFRYNGKLQPVTDDPLTPQETQRLIFTMLNNSQRKQLEQNWELDCSYGVKGL
ARFRVNVYKERGCYAACLRALSSKIPNYEKLGLPDVVKEMAGRPRGLILVTGPTGSGKTTTLAAILDLINRTRHEHVLTV
EDPIEYVFPNEKCLFHQRQKGEDTKSFANALKAALREDPDIILVGELRDLETISLAVSAAETGHLVFGTLHTSSAAATVD
RMVDVFTAEQQEQIRAQLSGSLIAVFSQCLPKKQNPKPGEFGRALAQEIMVVTPAIANQIREGKTSQIYSSIQTGMKLGM
QTMEQSLADWVNKGVISMEEALSKSSKPDELYRLTSGASGKAKVKANARR

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=55053 PCC7418_RS05280 WP_015225139.1 1148573..1149685(-) (pilT) [Halothece sp. PCC 7418]
ATGGATTTAATGATCGAAGACTTGATGGAACAACTGGTGGAAAAAGGAGGCTCGGATATGCACATTCAAGCTGGTGCACC
AGTTTATTTCCGCTATAACGGGAAACTGCAACCCGTAACCGATGACCCCCTTACTCCCCAAGAAACCCAACGGCTGATTT
TCACGATGCTTAATAATAGCCAACGGAAACAGTTAGAGCAAAACTGGGAATTAGATTGTTCTTATGGGGTGAAAGGACTG
GCTCGCTTTCGGGTTAATGTCTATAAAGAACGGGGTTGTTATGCTGCTTGCTTACGGGCTCTCTCCTCAAAAATTCCTAA
CTACGAAAAGTTAGGATTACCCGATGTAGTGAAAGAAATGGCAGGTCGTCCCCGTGGTTTAATCTTAGTAACAGGACCGA
CGGGGTCTGGGAAAACAACCACACTTGCTGCCATTTTAGACTTAATTAATCGCACCCGTCATGAGCACGTTTTAACCGTT
GAAGATCCTATTGAGTATGTTTTTCCCAATGAAAAATGTCTCTTTCACCAACGGCAAAAAGGGGAAGACACAAAGAGTTT
TGCCAATGCCTTAAAAGCAGCGTTGCGGGAAGACCCTGATATCATCCTCGTGGGAGAACTACGGGACTTAGAAACGATTT
CTCTCGCCGTATCTGCAGCAGAAACGGGTCACTTAGTATTTGGAACATTACACACCAGTTCGGCAGCAGCAACCGTGGAT
CGGATGGTGGATGTGTTCACTGCCGAACAACAAGAGCAAATCCGAGCACAGTTGTCTGGATCTCTGATTGCAGTGTTTAG
TCAATGTTTACCGAAGAAACAAAATCCCAAACCCGGTGAATTTGGACGCGCCTTAGCCCAAGAAATTATGGTGGTCACTC
CCGCGATCGCGAACCAAATTCGAGAAGGAAAAACCTCACAAATTTATTCTTCCATCCAAACGGGAATGAAACTGGGAATG
CAGACCATGGAACAGTCTCTTGCCGATTGGGTGAATAAAGGGGTGATTTCTATGGAAGAAGCTCTCTCGAAAAGTAGTAA
GCCCGATGAACTCTATCGCTTAACTTCGGGAGCATCCGGGAAAGCAAAAGTTAAAGCCAATGCACGTCGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-258)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K9Y9W6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain Lp02

53.043

93.243

0.495
  pilT Legionella pneumophila strain ERS1305867

53.043

93.243

0.495
  pilT Pseudomonas aeruginosa PAK

51.009

93.784

0.478
  pilT Acinetobacter nosocomialis M2

50.144

93.784

0.47
  pilT Acinetobacter baumannii D1279779

50.144

93.784

0.47
  pilT Acinetobacter baumannii strain A118

50.144

93.784

0.47
  pilT Pseudomonas stutzeri DSM 10701

49.856

93.784

0.468
  pilT Acinetobacter baylyi ADP1

49.856

93.784

0.468
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.267

92.162

0.454
  pilT Vibrio cholerae strain A1552

49.267

92.162

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.761

95.946

0.449
  pilT Neisseria gonorrhoeae MS11

45.845

94.324

0.432
  pilT Neisseria meningitidis 8013

45.845

94.324

0.432
  pilU Pseudomonas stutzeri DSM 10701

38.617

93.784

0.362

Multiple sequence alignment