Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   PCC7418_RS05285 Genome accession   NC_019779
Coordinates   1149914..1151938 (-) Length   674 a.a.
NCBI ID   WP_015225140.1    Uniprot ID   K9YB00
Organism   Halothece sp. PCC 7418     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1144914..1156938
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCC7418_RS05260 (PCC7418_1050) - 1144917..1145447 (-) 531 WP_015225135.1 TIGR02281 family clan AA aspartic protease -
  PCC7418_RS05265 (PCC7418_1051) ald 1145578..1146660 (+) 1083 WP_015225136.1 alanine dehydrogenase -
  PCC7418_RS05270 (PCC7418_1052) - 1146719..1147288 (-) 570 WP_015225137.1 hypothetical protein -
  PCC7418_RS05275 (PCC7418_1053) - 1147314..1148534 (-) 1221 WP_015225138.1 type II secretion system F family protein -
  PCC7418_RS05280 (PCC7418_1054) pilT 1148573..1149685 (-) 1113 WP_015225139.1 type IV pilus twitching motility protein PilT Machinery gene
  PCC7418_RS05285 (PCC7418_1055) pilB/pilB1 1149914..1151938 (-) 2025 WP_015225140.1 GspE/PulE family protein Machinery gene
  PCC7418_RS05295 (PCC7418_1056) bchL 1152744..1153622 (+) 879 WP_015225141.1 ferredoxin:protochlorophyllide reductase (ATP-dependent) iron-sulfur ATP-binding protein -
  PCC7418_RS05300 (PCC7418_1057) - 1153713..1155131 (+) 1419 WP_015225142.1 ferredoxin:protochlorophyllide reductase (ATP-dependent) subunit N -
  PCC7418_RS19730 (PCC7418_1058) petN 1155475..1155564 (+) 90 WP_015225143.1 cytochrome b6-f complex subunit PetN -
  PCC7418_RS20430 - 1155641..1155850 (+) 210 WP_171814888.1 hypothetical protein -
  PCC7418_RS05305 (PCC7418_1060) - 1155847..1156746 (-) 900 WP_015225144.1 S1 RNA-binding domain-containing protein -

Sequence

Protein


Download         Length: 674 a.a.        Molecular weight: 75734.19 Da        Isoelectric Point: 4.7295

>NTDB_id=55054 PCC7418_RS05285 WP_015225140.1 1149914..1151938(-) (pilB/pilB1) [Halothece sp. PCC 7418]
MTYSSSAKKRQRALTTLRDSSPFGNKLIQAGYASSKEIEQARQQVTQSEGKQSFTEALSEVTGKELPPELYRQYKKHHLF
ELKIYYGVESLDPEVTDLEEYPIAELIDDIIPLDLCRRHKLLPLSKRDTRPPSLLVGMVNPDELTAQDDLTRILRPKGFI
PQRVVMTLDDYERIIGKYLDEQSAQQEEAEEEDQLDVTKDIANLDMEGLEEVNDDTDADLEKSLGDAQAAPIINLVNRIL
IKALKDGVSDIHIEPQEEYVRVRMRRDGVLQETFDNLPIKISPAIAARFKIMAELDIAERRMPQDGKIRRKYKGRNVDFR
VSTLPSRYGEKIVLRILDNSATQLGLDKLITDQKSLDLVRDMASRPFGLILVTGPTGSGKSTTLYSILAERNDPGVNIST
AEDPIEYALPGITQVQVIREKGMDFASILRSFLRQDPDIILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAAGAIARL
DEMGVEPFMISGSLIGVLAQRLMRRVCSQCRIPYNPTQEELARFGLSATKEEEVTFYKANTLQPDEIEQARKQNKLCSKC
NGVGYKGRAGVYEIMAMSETLQMLINEGAPTERIKEAAVEEGMQTLLAYSLNLVREGYTTLDEVERVTFTDSGLEAELKA
KRKSSLTCNTCSAELQPEWLDCPYCLTPRFQDNE

Nucleotide


Download         Length: 2025 bp        

>NTDB_id=55054 PCC7418_RS05285 WP_015225140.1 1149914..1151938(-) (pilB/pilB1) [Halothece sp. PCC 7418]
ATGACTTATTCCTCATCTGCCAAAAAACGTCAAAGAGCCCTAACCACATTACGTGACTCCTCCCCATTTGGGAATAAATT
GATTCAAGCTGGCTATGCTAGTAGTAAGGAAATTGAACAAGCCAGACAACAAGTTACTCAATCAGAAGGCAAGCAATCTT
TTACCGAAGCGCTGAGTGAAGTTACTGGTAAAGAATTACCGCCTGAACTCTATCGTCAGTACAAAAAACATCATTTATTT
GAATTAAAAATTTATTATGGGGTCGAGTCTCTCGATCCAGAGGTTACAGATCTCGAAGAGTATCCGATTGCAGAATTGAT
TGATGACATTATCCCCCTCGACCTCTGTCGTCGTCACAAGTTGCTGCCTTTGTCCAAGCGAGACACCCGCCCTCCATCCT
TGTTAGTGGGAATGGTCAATCCAGATGAACTGACCGCCCAAGATGATCTAACGCGCATTTTACGCCCCAAAGGGTTTATC
CCTCAGCGAGTGGTGATGACGTTGGACGATTATGAACGAATTATTGGCAAATATTTGGATGAGCAATCGGCGCAGCAAGA
AGAAGCGGAAGAAGAGGATCAACTTGATGTTACGAAAGACATCGCTAATCTTGATATGGAAGGGCTGGAAGAGGTCAATG
ACGATACAGATGCTGACCTAGAAAAGTCATTGGGAGATGCACAAGCAGCGCCGATTATTAACTTAGTGAATCGGATTTTA
ATTAAAGCCCTCAAGGATGGAGTTTCTGATATTCATATTGAGCCTCAAGAAGAATATGTGAGGGTGCGGATGCGCCGTGA
TGGTGTTCTTCAAGAAACCTTTGACAACTTACCGATTAAAATTTCTCCCGCGATCGCGGCTCGCTTTAAAATTATGGCTG
AGTTAGATATTGCCGAACGCAGGATGCCTCAAGATGGCAAGATTCGTCGGAAATATAAAGGGCGTAATGTGGATTTTCGG
GTGAGCACGCTTCCCAGTCGTTATGGGGAAAAGATTGTACTACGGATTTTAGATAACAGTGCGACGCAACTGGGACTGGA
TAAATTAATTACAGACCAGAAAAGTTTGGATCTCGTCAGGGATATGGCGAGTCGCCCCTTTGGTCTGATTCTGGTGACTG
GACCCACGGGTTCGGGGAAATCAACCACCTTGTATTCGATTCTCGCCGAGCGCAATGACCCAGGGGTGAATATTAGCACC
GCAGAAGACCCCATTGAATATGCCTTACCGGGGATTACCCAGGTGCAGGTGATTCGGGAAAAAGGGATGGATTTTGCTTC
CATCTTACGGTCATTTCTCCGTCAAGACCCTGATATTATTCTGGTGGGGGAAACCCGAGACAAAGAAACCGCAAAAACAG
CAATTGAAGCTGCTTTAACGGGACACTTGGTTTTAACGACCTTACACACCAATGATGCTGCGGGCGCGATCGCGCGTTTA
GATGAAATGGGAGTAGAACCCTTTATGATTTCCGGTTCGTTAATTGGTGTTCTTGCCCAACGTTTAATGCGTCGGGTGTG
TAGTCAATGTCGTATCCCATACAACCCGACTCAAGAGGAACTGGCTCGGTTTGGTTTATCCGCAACCAAAGAAGAAGAAG
TCACCTTCTACAAAGCCAATACCCTACAACCCGATGAAATTGAACAAGCCCGAAAGCAAAATAAATTGTGTAGTAAATGT
AACGGCGTTGGTTATAAAGGGCGTGCTGGGGTCTATGAAATCATGGCAATGAGTGAAACCTTACAAATGCTAATTAACGA
AGGGGCCCCCACAGAAAGAATCAAAGAAGCTGCGGTTGAGGAAGGGATGCAAACACTACTAGCTTATAGTTTGAATCTGG
TGCGAGAAGGATACACCACCTTAGACGAAGTAGAACGGGTCACCTTTACCGACTCGGGGCTAGAAGCAGAACTGAAAGCG
AAACGGAAGAGTTCACTCACTTGTAATACTTGCTCAGCAGAACTGCAACCCGAATGGTTAGATTGTCCTTATTGCTTGAC
CCCTCGTTTCCAAGACAATGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(238-505)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(76-184)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K9YB00

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

66.216

98.813

0.654

Multiple sequence alignment