Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SYN6312_RS02645 Genome accession   NC_019680
Coordinates   551001..552098 (-) Length   365 a.a.
NCBI ID   WP_015123318.1    Uniprot ID   K9RR71
Organism   Synechococcus sp. PCC 6312     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 546001..557098
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SYN6312_RS02615 (Syn6312_0545) pyrF 546425..547150 (+) 726 WP_015123312.1 orotidine-5'-phosphate decarboxylase -
  SYN6312_RS02620 (Syn6312_0546) thiL 547181..548173 (-) 993 WP_015123313.1 thiamine-phosphate kinase -
  SYN6312_RS02625 (Syn6312_0547) - 548254..548706 (-) 453 WP_015123314.1 hypothetical protein -
  SYN6312_RS02630 (Syn6312_0548) - 548777..549040 (-) 264 WP_015123315.1 DUF3143 domain-containing protein -
  SYN6312_RS02635 (Syn6312_0549) - 549112..549564 (-) 453 WP_015123316.1 J domain-containing protein -
  SYN6312_RS02640 (Syn6312_0550) - 549710..550930 (-) 1221 WP_015123317.1 type II secretion system F family protein -
  SYN6312_RS02645 (Syn6312_0551) pilT 551001..552098 (-) 1098 WP_015123318.1 type IV pilus twitching motility protein PilT Machinery gene
  SYN6312_RS02650 (Syn6312_0552) pilB/pilB1 552191..554203 (-) 2013 WP_015123319.1 GspE/PulE family protein Machinery gene
  SYN6312_RS02655 (Syn6312_0553) grpE 554452..555183 (+) 732 WP_015123320.1 nucleotide exchange factor GrpE -

Sequence

Protein


Download         Length: 365 a.a.        Molecular weight: 40258.37 Da        Isoelectric Point: 7.1077

>NTDB_id=54686 SYN6312_RS02645 WP_015123318.1 551001..552098(-) (pilT) [Synechococcus sp. PCC 6312]
MELMIEDLMEQVVASGGSDLHLSAGLPPYIRISGKLTPTEYEPMTPEQCQRLIFSMLNNTQRKHLEQNWELDCSYGVRGL
ARFRVNVYKDRGTYAACLRALSSKIPTFEQLGLPNIVREMTERPRGLILVTGPTGSGKTTTLAAMIDLINKTRAEHILTV
EDPIEFVYEPIKSLIHQRQVGEDTKNFANALRAALRQDPDIILVGEMRDLETIQLAISAAETGHLVFGTLHTSSAPQTVD
RMVDVFPPAQQTQIRVQLSNSLVAVFSQTLVPKKNPKPGEFGRVMAQEIMITTPAISNLIREGKTSQIYSAIQTGGKLGM
QTLEKVLADYYKSGMISYEAAISKSSRADELQRLIGSGVAAAGMH

Nucleotide


Download         Length: 1098 bp        

>NTDB_id=54686 SYN6312_RS02645 WP_015123318.1 551001..552098(-) (pilT) [Synechococcus sp. PCC 6312]
ATGGAATTAATGATCGAAGACTTAATGGAGCAGGTGGTGGCCAGCGGTGGTTCTGATTTACACCTCTCGGCTGGGTTGCC
CCCCTACATTCGGATTAGCGGCAAATTAACACCGACTGAGTATGAGCCGATGACTCCGGAACAATGCCAGCGGTTAATTT
TCAGTATGCTCAACAATACGCAACGGAAGCATCTTGAGCAGAACTGGGAATTGGACTGCTCCTATGGGGTGCGGGGCCTG
GCCCGGTTTCGGGTCAATGTCTATAAGGATCGAGGCACTTATGCGGCTTGCTTGCGGGCCCTCAGTTCTAAAATTCCCAC
CTTTGAGCAACTGGGCTTACCTAATATTGTTCGGGAAATGACTGAGCGACCGAGGGGGCTGATCTTGGTGACGGGCCCAA
CGGGATCGGGAAAAACCACAACCCTAGCGGCCATGATCGATCTGATCAACAAAACCCGAGCCGAACATATTTTGACTGTG
GAAGACCCGATTGAATTTGTCTATGAACCAATTAAAAGCTTAATTCACCAACGCCAAGTGGGAGAGGATACTAAGAATTT
TGCCAATGCCTTACGGGCGGCGTTACGGCAAGACCCCGATATTATTTTGGTAGGAGAGATGCGGGATTTGGAAACGATTC
AGTTGGCGATTTCCGCTGCAGAAACGGGGCACTTAGTCTTTGGAACGTTACACACGAGTTCGGCTCCCCAAACCGTGGAT
CGGATGGTGGATGTTTTTCCCCCGGCCCAGCAAACTCAGATTCGGGTGCAGTTATCCAACTCCTTGGTGGCCGTCTTCAG
CCAAACCTTGGTTCCTAAGAAAAATCCTAAACCAGGGGAGTTCGGCCGGGTCATGGCTCAGGAAATTATGATCACGACTC
CAGCTATTTCTAACCTGATTCGGGAAGGGAAAACTTCTCAGATTTACTCCGCGATTCAAACCGGGGGCAAATTGGGGATG
CAAACCCTGGAGAAAGTCTTGGCGGATTATTATAAATCGGGGATGATTAGCTACGAAGCGGCTATTTCCAAGTCGTCTCG
GGCAGATGAGTTGCAGCGGTTAATTGGCAGTGGAGTTGCAGCGGCCGGCATGCATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(34-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K9RR71

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

54.839

93.425

0.512
  pilT Pseudomonas stutzeri DSM 10701

53.89

95.068

0.512
  pilT Acinetobacter baylyi ADP1

54.227

93.973

0.51
  pilT Legionella pneumophila strain Lp02

53.043

94.521

0.501
  pilT Legionella pneumophila strain ERS1305867

53.043

94.521

0.501
  pilT Acinetobacter baumannii strain A118

53.412

92.329

0.493
  pilT Acinetobacter nosocomialis M2

53.412

92.329

0.493
  pilT Acinetobacter baumannii D1279779

53.412

92.329

0.493
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.025

98.356

0.482
  pilT Neisseria gonorrhoeae MS11

48.711

95.616

0.466
  pilT Neisseria meningitidis 8013

48.711

95.616

0.466
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.706

93.151

0.463
  pilT Vibrio cholerae strain A1552

49.706

93.151

0.463
  pilU Pseudomonas stutzeri DSM 10701

38.781

98.904

0.384

Multiple sequence alignment