Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   SYN6312_RS02650 Genome accession   NC_019680
Coordinates   552191..554203 (-) Length   670 a.a.
NCBI ID   WP_015123319.1    Uniprot ID   K9RP93
Organism   Synechococcus sp. PCC 6312     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 547191..559203
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SYN6312_RS02625 (Syn6312_0547) - 548254..548706 (-) 453 WP_015123314.1 hypothetical protein -
  SYN6312_RS02630 (Syn6312_0548) - 548777..549040 (-) 264 WP_015123315.1 DUF3143 domain-containing protein -
  SYN6312_RS02635 (Syn6312_0549) - 549112..549564 (-) 453 WP_015123316.1 J domain-containing protein -
  SYN6312_RS02640 (Syn6312_0550) - 549710..550930 (-) 1221 WP_015123317.1 type II secretion system F family protein -
  SYN6312_RS02645 (Syn6312_0551) pilT 551001..552098 (-) 1098 WP_015123318.1 type IV pilus twitching motility protein PilT Machinery gene
  SYN6312_RS02650 (Syn6312_0552) pilB/pilB1 552191..554203 (-) 2013 WP_015123319.1 GspE/PulE family protein Machinery gene
  SYN6312_RS02655 (Syn6312_0553) grpE 554452..555183 (+) 732 WP_015123320.1 nucleotide exchange factor GrpE -
  SYN6312_RS02660 (Syn6312_0554) - 555446..557252 (+) 1807 Protein_532 Hsp70 family protein -
  SYN6312_RS02665 (Syn6312_0555) dnaJ 557270..558406 (+) 1137 WP_015123321.1 molecular chaperone DnaJ -
  SYN6312_RS02670 (Syn6312_0556) - 558403..558645 (+) 243 WP_015123322.1 sulfurtransferase TusA family protein -

Sequence

Protein


Download         Length: 670 a.a.        Molecular weight: 74201.15 Da        Isoelectric Point: 5.1027

>NTDB_id=54687 SYN6312_RS02650 WP_015123319.1 552191..554203(-) (pilB/pilB1) [Synechococcus sp. PCC 6312]
MVNTPPQRRALTVRRHDTPTQKALVESGYASSEQVREAMAESRKSGRSLVQVLQDVTGRAMPPDILRQYKKQQLFELKVI
YGIDCLDPDLNRFPTDQIGQLIGTVLAIDICRTYQAIPISMNRESDPPYLLVAMVDPDNLEAVDNLGKALRGQGLTLRRM
VITLEDYQHLITPFLEKQLQEESVKTGPAALGAIDLEEDLDAIGGLDDADGEQEVDLVDALKGAEDAPIIALVNKVLAKA
LQDGVSDIHIEPQEEFLRIRFRKDGVLQQAFDPLPKKIIPAVVSRFKILADLDIAERRAPQDGRIRKIFQGRRIDFRVNT
LPSRWGEKVVLRILDNSSTQLGLDKLISDPESLAIVRDMTKRPFGLILVTGPTGSGKTTTLYSALAECNSPGVNISTAED
PIEYTLPGLTQVQVIREKNMNFSEILRAFLRQDPDVILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAASAVARLSEM
EVEPFMVSASLIGVVAQRLMRRVCSECRIPYQPTREELARFGLSSSQSLQATFYKANRLTAEEIQVAKASGAPLCSKCGG
IGYKGRIGVYEILRVTERLQSLITEAAPTEQIKEAAVEEGMKTLLAYSLQLVQEGHTTFEEVERVTFTDTGLESELKAKR
KSALTCRVCSAGLQQEWLDCPFCMTPRFES

Nucleotide


Download         Length: 2013 bp        

>NTDB_id=54687 SYN6312_RS02650 WP_015123319.1 552191..554203(-) (pilB/pilB1) [Synechococcus sp. PCC 6312]
ATGGTCAATACCCCTCCCCAACGACGTGCTTTAACCGTGCGGCGGCATGATACCCCGACCCAAAAAGCTTTGGTTGAGTC
CGGTTATGCCAGTTCTGAACAAGTCAGGGAGGCGATGGCCGAAAGTCGGAAATCGGGACGATCCCTGGTACAGGTACTCC
AAGATGTCACTGGGCGAGCGATGCCCCCAGATATTTTACGTCAATATAAAAAGCAGCAATTATTTGAACTAAAAGTCATT
TACGGTATTGACTGTCTTGATCCGGACTTGAATCGCTTTCCCACAGATCAAATTGGGCAGCTAATTGGGACGGTGTTGGC
TATCGATATTTGTCGCACCTACCAGGCCATCCCAATTTCTATGAACCGGGAAAGTGATCCCCCCTATCTGCTAGTGGCGA
TGGTGGATCCGGATAATTTGGAAGCGGTGGATAACTTAGGGAAGGCGTTACGGGGGCAGGGCCTGACGCTCCGGCGGATG
GTCATCACTCTTGAAGACTATCAACACCTGATCACCCCATTTCTTGAAAAGCAACTCCAAGAGGAATCCGTTAAAACTGG
CCCGGCGGCCTTGGGGGCTATTGATCTTGAGGAAGACTTGGATGCCATTGGGGGCCTGGATGATGCTGATGGGGAGCAGG
AAGTTGACCTTGTTGATGCCCTCAAAGGTGCTGAAGATGCCCCAATTATTGCCTTGGTCAACAAAGTCTTAGCCAAAGCC
TTACAGGATGGAGTTTCGGATATTCATATTGAACCCCAAGAAGAGTTTTTACGGATTCGCTTTCGCAAGGATGGGGTTCT
TCAACAGGCCTTTGATCCCCTACCTAAGAAAATTATCCCAGCCGTAGTTTCTCGCTTTAAAATTCTGGCAGATTTAGATA
TTGCAGAACGGCGGGCCCCCCAAGATGGCCGGATCCGCAAGATATTCCAGGGGCGGCGGATTGATTTCCGGGTCAACACT
CTACCCAGTCGTTGGGGCGAAAAGGTTGTCCTTCGGATTTTGGATAACTCCTCTACCCAACTGGGCCTGGATAAATTGAT
TTCCGATCCTGAGAGCTTAGCCATTGTCCGGGATATGACCAAGCGACCCTTCGGATTAATTCTGGTCACGGGGCCAACGG
GTTCGGGAAAAACGACCACGCTCTATTCGGCCTTGGCCGAGTGTAACAGCCCCGGTGTCAACATCAGTACGGCGGAAGAC
CCGATTGAATATACCCTACCTGGCCTGACCCAGGTGCAGGTGATTCGGGAAAAAAATATGAACTTTTCCGAAATTCTCCG
AGCCTTCTTGCGGCAAGACCCGGATGTGATCCTGGTGGGGGAAACTCGGGACAAAGAAACCGCTAAAACCGCCATTGAAG
CTGCTTTGACTGGGCACTTAGTTTTAACCACGCTCCATACCAATGATGCAGCCAGTGCCGTTGCTCGACTCTCAGAAATG
GAAGTGGAGCCCTTTATGGTTTCAGCCTCCTTAATTGGGGTGGTGGCCCAGCGGTTAATGCGGCGGGTCTGTTCCGAATG
TCGGATTCCCTATCAACCCACTCGGGAAGAACTGGCTCGGTTTGGCTTATCCAGTTCCCAGAGCTTACAAGCTACCTTTT
ATAAAGCCAACCGTCTCACGGCAGAAGAGATTCAGGTAGCAAAGGCATCTGGTGCACCACTGTGTAGCAAATGTGGGGGG
ATTGGCTATAAAGGGCGGATCGGGGTCTATGAAATTCTCCGGGTGACAGAACGACTGCAATCTCTGATTACTGAAGCAGC
CCCTACAGAGCAAATTAAGGAAGCTGCCGTTGAAGAGGGGATGAAAACCTTGCTGGCTTATAGTTTGCAACTGGTTCAAG
AAGGTCATACCACCTTTGAAGAAGTGGAGCGGGTGACCTTTACTGATACCGGCCTGGAGTCGGAACTCAAGGCTAAACGC
AAAAGTGCCCTGACATGTCGCGTCTGTAGCGCCGGACTACAGCAGGAGTGGTTAGACTGTCCCTTCTGTATGACTCCTCG
TTTTGAGAGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(235-502)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(24-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K9RP93

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

64.253

98.955

0.636

Multiple sequence alignment